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Citations for System Biosciences :
1 - 29 of 29 citations for 8 Bromo 5 6 difluoro 2 methylquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... ARN8 HBEGFKO cells were transfected at 80% confluence in a 6-well plate with pCMV-hHBEGF (2 µg) and PB Transposase vector (0.5 µg) (System Biosciences) using FuGENE® HD (Promega ...
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bioRxiv - Immunology 2021Quote: ... 8×106 293TN Producer cells (System Biosciences, Cat # LV900A-1) were plated in T150 flasks overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... 8 pl of a mixture containing 500 ng/μl RFP-Piranha mRNA (System Biosciences) and 0.1 mg/ml anti-Tdrd3 antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Genomics 2023Quote: 16 million K562 cells (8 million K562-VP64 cells and 8 million K562-VPR cells) were transfected with the gRNA library and the piggyBac transposase (System Biosciences, Cat. No. PB210PA-1) at a 20:1 molar ratio of library:transposase using a Lonza 4D nucleofector and the Lonza nucleofector protocol for K562 cells ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
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bioRxiv - Genetics 2019Quote: ... matched with Zfr knockdown: 5’-AACCGATGTACTTCCCGTTAAT) were cloned into the pGreenPuro backbone from System Biosciences. This construct was used to produce a 6-well of lentivirus according to standard protocols in 293T cells using the third-generation system and concentrated 1:100 with lenti-X (Clontec) ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2021Quote: ... mRuby was amplified from PB-EF1α-MCS-IRES-RFP (System Biosciences PB531A-2) by PCR ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2021Quote: ... media were changed to DMEM supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The corresponding oligos were ordered from Twist BioScience and cloned into an untagged (System BioSciences, PB533A-2), GFP-tagged (pPB_EF1_MCS-EGFP_IRES_Puro ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Cell Biology 2022Quote: ... one day before the transfection of the expression construct of mCherry-proliferating-cell nuclear antigen (PCNA) or EGFP-ATM based on PB533A-2 (System Biosciences) with a PiggyBac transposon expression vector (PB210PA-1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and PB-SIN-puro include the backbone and ITR and insulator domains of the piggybac transposon vector PB-EF1α-MCS-IRES-GFP (System Biosciences PB530A-2) and contain a 5’ compound SV40 enhancer with hybrid RSV-MMSV LTR (based on SERS11 design66) ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the cloning site with Kozak sequence fragment #1 was amplified from the PB-EF1-MCS-IRES-Neo vector (PB533A-2; System Biosciences, Palo Alto, CA, USA) using PCR and the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).