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Citations for System Biosciences :
1 - 38 of 38 citations for 6 Bromo 2 trifluoromethyl 3H imidazo 4 5 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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bioRxiv - Microbiology 2021Quote: ... ARN8 HBEGFKO cells were transfected at 80% confluence in a 6-well plate with pCMV-hHBEGF (2 µg) and PB Transposase vector (0.5 µg) (System Biosciences) using FuGENE® HD (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Cell Biology 2019Quote: ... The purity of exosome fraction was also evaluated with Exosome Array (System Biosciences, EXORAY-4).
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bioRxiv - Bioengineering 2022Quote: ... and Exo-Check™ exosome antibody array (EXORAY200B-4) were purchased from SBI (System Biosciences). miRCURY LNA™ RT kit ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... matched with Zfr knockdown: 5’-AACCGATGTACTTCCCGTTAAT) were cloned into the pGreenPuro backbone from System Biosciences. This construct was used to produce a 6-well of lentivirus according to standard protocols in 293T cells using the third-generation system and concentrated 1:100 with lenti-X (Clontec) ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2021Quote: ... mRuby was amplified from PB-EF1α-MCS-IRES-RFP (System Biosciences PB531A-2) by PCR ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2021Quote: ... media were changed to DMEM supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
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bioRxiv - Molecular Biology 2023Quote: ... The corresponding oligos were ordered from Twist BioScience and cloned into an untagged (System BioSciences, PB533A-2), GFP-tagged (pPB_EF1_MCS-EGFP_IRES_Puro ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Synthetic Biology 2021Quote: ... A total of 4 μg of piggyBac targeting base editor plasmids were transfected with 1 μg of super transposase plasmid (SBI System Biosciences cat# PB210PA-1) using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Molecular Biology 2020Quote: An amount of 4 ng of XMIRXpress vector containing Non-Targeting miRNA with Xmotif (cat. no. XMIRXP-NT, System Biosciences LLC, Palo Alto, CA, USA) was introduced into 100 μL Stellar competent E ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Cell Biology 2022Quote: ... one day before the transfection of the expression construct of mCherry-proliferating-cell nuclear antigen (PCNA) or EGFP-ATM based on PB533A-2 (System Biosciences) with a PiggyBac transposon expression vector (PB210PA-1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and PB-SIN-puro include the backbone and ITR and insulator domains of the piggybac transposon vector PB-EF1α-MCS-IRES-GFP (System Biosciences PB530A-2) and contain a 5’ compound SV40 enhancer with hybrid RSV-MMSV LTR (based on SERS11 design66) ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the cloning site with Kozak sequence fragment #1 was amplified from the PB-EF1-MCS-IRES-Neo vector (PB533A-2; System Biosciences, Palo Alto, CA, USA) using PCR and the following primers ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).