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Citations for Spherotech :
1 - 19 of 19 citations for Recombinant Human Carboxylesterase 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... diluted to ~20 nm was mixed with ~1 pm of penta-His-biotin antibody conjugated streptavidin polystyrene beads (0.43 μm or 0.56 μm or 0.89 μm diameter, Spherotech). This ratio produced the bead binding fraction of 10-15% and was maintained to maximize the probability of finding single motor beads in the solution ...
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bioRxiv - Developmental Biology 2023Quote: ... the procedure was the same as described above despite that His-beads were substituted by Streptavidin Magnetic Particles (Spherotech).
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bioRxiv - Genetics 2022Quote: Recombinant His6-tag DASH complex was linked to 0.56 μm-diameter streptavidin-coated polystyrene microbeads (Spherotech) using biotinylated His5-antibody (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Cell Biology 2023Quote: 3 μm carboxyl polystyrene beads (Spherotech, CP30-10) were covalently coupled with purified human IgG (hIgG ...
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bioRxiv - Biophysics 2023Quote: ... we coated streptavidin beads (3 µm in diameter, Spherotech) with fibronectin ...
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bioRxiv - Immunology 2020Quote: ... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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bioRxiv - Cell Biology 2022Quote: 3 μm carboxyl polystyrene microspheres (Spherotech/ Cat No. CP-30-10) were covalently coupled with hIgG by washing 3 times in sterile PBS at maximum speed (18,000 g ...
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bioRxiv - Cell Biology 2022Quote: Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
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bioRxiv - Synthetic Biology 2019Quote: ... Laser alignment was checked using 3 µm Ultra Rainbow fluorescent particles (Spherotech CAT# RCP-30-5A). Cytometer settings were maintained between WT and ex-conjugate P ...
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bioRxiv - Biophysics 2022Quote: ... we additionally relied on 3 μm diameter beads (1.0% w/v, Spherotech, cat. SVSIP-30-5).
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bioRxiv - Immunology 2023Quote: In-channel IgG capture assays were performed using single assay mixtures containing anti-human IgG-coated 6-8μm beads (Spherotech) and fluorescently-labeled detection anti-IgG antibodies (2.5μg/mL ...
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bioRxiv - Biophysics 2021Quote: ... 5 nM 3’ overhand handles were mixed with 0.08% w/v streptavidin-coated polystyrene beads (2.1 μm diameter, Spherotech) in optical tweezers assay buffer ...
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bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
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bioRxiv - Biophysics 2019Quote: The 8.1 kbp dsDNA construct with a primer-template junction at one terminus was tethered between 2 μm anti-digoxigenin and 3 μm streptavidin functionalized beads (Spherotech) held in place by a micropipette tip and a dual beam optical trap ...
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bioRxiv - Plant Biology 2019Quote: ... Nanophytoplankton were distinguished from picoplankton based on light scatter (FSC and SSC) and pulse width (transit time) compared to 3 µm UltraRainbow fluorescent particles (Spherotech).
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bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...