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Citations for Spherotech :
1 - 26 of 26 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Cell Biology 2021Quote: Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
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bioRxiv - Immunology 2021Quote: ... and cell numbers were quantified using PE-conjugated fluorescent counting beads (Spherotech).
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bioRxiv - Microbiology 2020Quote: ... and cell numbers were quantified using PE-conjugated fluorescent counting beads (Spherotech).
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bioRxiv - Bioengineering 2023Quote: ... Pre-rinsed 4.5 µm streptavidin labeled polystyrene particles (Spherotech SVP-40-5) are combined with 1 µM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... diluted to ~20 nm was mixed with ~1 pm of penta-His-biotin antibody conjugated streptavidin polystyrene beads (0.43 μm or 0.56 μm or 0.89 μm diameter, Spherotech). This ratio produced the bead binding fraction of 10-15% and was maintained to maximize the probability of finding single motor beads in the solution ...
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bioRxiv - Developmental Biology 2023Quote: ... the procedure was the same as described above despite that His-beads were substituted by Streptavidin Magnetic Particles (Spherotech).
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bioRxiv - Genetics 2022Quote: Recombinant His6-tag DASH complex was linked to 0.56 μm-diameter streptavidin-coated polystyrene microbeads (Spherotech) using biotinylated His5-antibody (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
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bioRxiv - Immunology 2021Quote: Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens ...
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bioRxiv - Immunology 2022Quote: Streptavidin beads pre-labelled with individual intensities of PE-channel fluorophore (Spherotech, SVFA-2558-6K and SVFB-2558-6K) or FITC-channel fluorophore (Spherotech ...
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bioRxiv - Immunology 2020Quote: ... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: The molecules of equivalent soluble fluorochrome (MESF) were calculated for PE and CFSE using SPERO TM Rainbow Beads Calibration Particles (Spherotech RCP-05-5, USA) according to instructions provided by the manufacturer ...
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bioRxiv - Biophysics 2021Quote: Protein G-coated polystyrene beads (Spherotech, PGP-60-5) were functionalized with PSGL1-Fc Chimera (R&D systems ...
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bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were purified with Neutradivin coated magnetic beads (Spherotech) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
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bioRxiv - Immunology 2023Quote: In-channel IgG capture assays were performed using single assay mixtures containing anti-human IgG-coated 6-8μm beads (Spherotech) and fluorescently-labeled detection anti-IgG antibodies (2.5μg/mL ...
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bioRxiv - Microbiology 2022Quote: Purified biotinylated proteins were multimerised on streptavidin coated Nile Red fluorescent beads (Spherotech Inc ...
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bioRxiv - Immunology 2022Quote: A 20 μg/mL solution of PhyB protein was added to washed streptavidin-coated polystyrene beads (5.0-5.9 μm, Spherotech), before a 2h incubation on ice ...
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Cell Biology 2019Quote: ... 100 nm of WT and mutant Spc105 proteins was incubated with 10 µl of 0.1% w/v streptavidin polystyrene beads (Spherotech) conjugated with anti-penta-his biotin antibody (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Biophysics 2022Quote: An aliquot of the crosslinked protein-DNA sample was incubated with 1 μL anti-digoxigenin coated polystyrene beads 2.17 μm in diameter (Spherotech, IL), diluted in 1 mL phosphate-buffered saline (PBS) ...
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bioRxiv - Biophysics 2023Quote: ... Measurements were performed using a dumbbell conformation: the protein of interest was attached in between two 1 μm-sized silica beads (Spherotech, US), using double stranded (ds ...