Labshake search
Citations for Spherotech :
1 - 32 of 32 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Nuclei counts were determined using an internal control bead population (Spherotech, Lake Forest, IL, USA) at 106 beads/mL ...
-
bioRxiv - Bioengineering 2020Quote: ... Compensation controls were generated using single-stained populations of positive beads and negative beads (Spherotech #CMIgP-30). Data analysis and compensation correction was performed using FlowJo software ...
-
bioRxiv - Cell Biology 2024Quote: ... Voltages were established using relevant controls and were maintained (+/- 10%) across replicates using calibration particles (Spherotech, URCP-38-2K). Cells were separated from debris by optical gating based off forward and side scatter area ...
-
bioRxiv - Immunology 2023Quote: ... 1 x 104 counting beads (Spherotech) were added to stained samples at the final step ...
-
bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
-
bioRxiv - Cell Biology 2020Quote: ... diluted to ~20 nm was mixed with ~1 pm of penta-His-biotin antibody conjugated streptavidin polystyrene beads (0.43 μm or 0.56 μm or 0.89 μm diameter, Spherotech). This ratio produced the bead binding fraction of 10-15% and was maintained to maximize the probability of finding single motor beads in the solution ...
-
bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
-
bioRxiv - Biophysics 2021Quote: ... 1~2 μL dilution of such streptavidin-DNA mixture containing 1~10 ng DNA was mixed with 10 μL anti-digoxigenin antibody coated polystyrene beads 2.1 μm in diameter (Spherotech) and incubated at room temperature for 15 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... constructs were mixed with 4 μl of polystyrene beads coated with antibodies against digoxigenin (AD beads, 0.1% v/v suspension, Ø 1.76 μm, Spherotech), 10 μl of assay buffer (20 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
-
bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
-
bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
-
bioRxiv - Immunology 2021Quote: ... The cells were screened in a 30 min time-course assay for secretion of antibodies that bound to 7 µm streptavidin beads (Spherotech) coated with 10 µg/mL SARS-CoV-2 spike or RBD ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were screened in a 30 min time course assay for secretion of antibodies that bound to streptavidin beads (Spherotech) coated with 10µg/mL biotinylated SARS-CoV-2 S-2P ...
-
bioRxiv - Biophysics 2021Quote: ... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
-
bioRxiv - Biochemistry 2019Quote: ... prior to binding of the complex to 1 μM diameter polystyrene beads (Spherotech) functionalized with an anti-digoxigenin antibody (Roche ...
-
bioRxiv - Biophysics 2021Quote: ... 50 μl of 1% w/v 800-nm streptavidin-coated polystyrene beads (Spherotech Inc) were washed three times in 200 μl 1x PBS buffer and centrifuged at 7500 rpm ...
-
bioRxiv - Neuroscience 2020Quote: ... microglia was incubated with ANXV (1:20) for 15’ and fluorescent particles (3um) (Spherotech) were added to microglial cells at a 1:1 microglia/beads ratio for 2hr ...
-
bioRxiv - Immunology 2021Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (16) ...
-
bioRxiv - Microbiology 2020Quote: ... Each cytometer run was calibrated with 1 mm diameter fluorescent Ultra Rainbow beads (Spherotech Inc.). 100 μL for autofluorescent cells and 75 μL for heterotrophic cells were run at an average flow rate of 20 μL min−1 and monitored with a liquid flowmeter (Sensirion US) ...
-
bioRxiv - Immunology 2020Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (Su et al. ...
-
bioRxiv - Immunology 2024Quote: ... Frequency calculation was obtained by mixing 1/10th of samples with 200,000 fluorescent beads (Spherotech) for normalization.
-
bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
-
bioRxiv - Biophysics 2023Quote: ... of ∼0.045 pN nm−1 while using the polystyrene bead of 0.56 μm (streptavidin conjugated, Spherotech).
-
bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
-
bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
-
bioRxiv - Bioengineering 2021Quote: ... One percent (w/v) stock solutions of 1-1.4μm high-intensity polystyrene Nile red fluorescent microparticles were procured from Spherotech Inc (FH-1056-2 ...
-
bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
-
bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
-
bioRxiv - Biophysics 2022Quote: An aliquot of the crosslinked protein-DNA sample was incubated with 1 μL anti-digoxigenin coated polystyrene beads 2.17 μm in diameter (Spherotech, IL), diluted in 1 mL phosphate-buffered saline (PBS) ...
-
bioRxiv - Biophysics 2023Quote: ... Measurements were performed using a dumbbell conformation: the protein of interest was attached in between two 1 μm-sized silica beads (Spherotech, US), using double stranded (ds ...
-
bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...