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Citations for Spherotech :
1 - 45 of 45 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: 3 μm carboxyl polystyrene microspheres (Spherotech/ Cat No. CP-30-10) were covalently coupled with hIgG by washing 3 times in sterile PBS at maximum speed (18,000 g ...
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bioRxiv - Cell Biology 2022Quote: Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
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bioRxiv - Synthetic Biology 2019Quote: ... Laser alignment was checked using 3 µm Ultra Rainbow fluorescent particles (Spherotech CAT# RCP-30-5A). Cytometer settings were maintained between WT and ex-conjugate P ...
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bioRxiv - Biophysics 2022Quote: ... we additionally relied on 3 μm diameter beads (1.0% w/v, Spherotech, cat. SVSIP-30-5).
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bioRxiv - Cell Biology 2023Quote: 3 μm carboxyl polystyrene beads (Spherotech, CP30-10) were covalently coupled with purified human IgG (hIgG ...
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bioRxiv - Biophysics 2023Quote: ... we coated streptavidin beads (3 µm in diameter, Spherotech) with fibronectin ...
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bioRxiv - Biophysics 2021Quote: ... 5 nM 3’ overhand handles were mixed with 0.08% w/v streptavidin-coated polystyrene beads (2.1 μm diameter, Spherotech) in optical tweezers assay buffer ...
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bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
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bioRxiv - Biophysics 2019Quote: The 8.1 kbp dsDNA construct with a primer-template junction at one terminus was tethered between 2 μm anti-digoxigenin and 3 μm streptavidin functionalized beads (Spherotech) held in place by a micropipette tip and a dual beam optical trap ...
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bioRxiv - Plant Biology 2019Quote: ... Nanophytoplankton were distinguished from picoplankton based on light scatter (FSC and SSC) and pulse width (transit time) compared to 3 µm UltraRainbow fluorescent particles (Spherotech).
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bioRxiv - Immunology 2024Quote: ... Avidin beads (Spherotech, SVP-30-5) were pre-incubated in the same medium with different concentrations of CD4- or CD8-biotin and washed once ...
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bioRxiv - Synthetic Biology 2020Quote: ... Rainbow Calibration Particles (Spherotech #RCP-30-5) or UltraRainbow Calibration Particles (Spherotech #URCP-100-2H ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8-peak beads (Spherotech, RCP-30-5A) were used to establish how fluorescence relates to standardized units of molecules of equivalent fluorophores (MEFL ...
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bioRxiv - Bioengineering 2019Quote: ... Rainbow fluorescent beads (Spherotech, Cat. # RFP-30-5A) were used as fluorescence intensity standards ...
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bioRxiv - Cell Biology 2023Quote: ... ∼3μm carboxyl-polystyrene beads (Spherotech, CP-30-10) were washed twice with 1X DPBS and activated in glass tubes (Pyrex ...
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bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fluorescent beads (Rainbow Calibration beads, Spherotech, RCP-30-5A) samples were also acquired during every flow cytometry experiment to enable conversion of data from arbitrary units (a.u. ...
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bioRxiv - Immunology 2021Quote: ... Standardized SPHERO rainbow beads (Spherotech, Cat#RFP-30-5A) were used to track and adjust PMTs over time ...
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bioRxiv - Immunology 2020Quote: ... Standardized SPHERO rainbow beads (Spherotech, Cat#RFP-30-5A) were used to track and adjust PMTs over time ...
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bioRxiv - Synthetic Biology 2022Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each batch of samples to facilitate calibration of flow cytometry data to molecules of equivalent fluorescein (MEF ...
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bioRxiv - Cell Biology 2022Quote: Carboxyl polystyrene microsphere (3.0 μm, Spherotech, CP-30-10) were opsonized by covalent coupling with hIgG ...
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bioRxiv - Cell Biology 2023Quote: ... BSA-Beads (for immunofluorescence, Spherotech; BP-30-5; ∼3μm) or Biotin-Beads (for lipidomics ...
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bioRxiv - Synthetic Biology 2024Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each flow cytometry plate to enable calibration of the flow cytometry data to molecules of equivalent fluorophore5–7.
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bioRxiv - Cell Biology 2023Quote: ... or Biotin-Beads (for lipidomics, Spherotech; TP-30-5; ∼3μm) served as control beads ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... with negative and high in separate vials (Spherotech, Cat# CMIgP-30-2K), combining one drop from each vial in equal ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 8-peak rainbow beads (Cat# RCP-30-5A, Lot AF01, Spherotech, USA) were cross-calibrated to PE MESF values on each cytometer at the same voltage/gain as the PE MESF beads were acquired at ...
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bioRxiv - Biophysics 2022Quote: ... 500 μL of 5.0% w/v 3.4 μm amino-polystyrene beads (AP-30-100, Spherotech) was centrifuged at 2337 g for five minutes ...
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bioRxiv - Immunology 2020Quote: ... laser voltages were standardized using SPHERO™ Ultra Rainbow Fluorescent Particles (Spherotech #URFP01-30-2K). Data from at least 10,000 single bacterial events were measured and analyzed by FlowJo software (v10.0.7).
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bioRxiv - Immunology 2020Quote: ... Routinely alignments were performed with Ultra Rainbow Fluorescent Particles 3.0 µm (Spherotech, URFP-30-2). The triggering threshold was applied to the side scatter on the 488-SSC channel and the relative applied voltage was determined by using size reference beads (Apogee flow systems ...
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bioRxiv - Bioengineering 2020Quote: ... Compensation controls were generated using single-stained populations of positive beads and negative beads (Spherotech #CMIgP-30). Data analysis and compensation correction was performed using FlowJo software ...
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bioRxiv - Synthetic Biology 2019Quote: ... fluorescence channel measurements were converted to units of MEFL using 8-peak rainbow beads (Spherotech RCP-30-5A) and using linear color translation and compensation between fluorophores ...
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bioRxiv - Molecular Biology 2023Quote: ... Amino-modified polystyrene beads (AP-30-10) with a nominal diameter of 3.4 µm were purchased from Spherotech Inc ...
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bioRxiv - Synthetic Biology 2020Quote: ... Arbitrary fluorescence units were converted to standardized molecules of equivalent fluorescein (MEFL) units using RCP-30-5A beads (Spherotech) and the TASBE pipeline process50 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 μL portion of each cell sample ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 µL portion of each cell sample ...
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bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
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bioRxiv - Synthetic Biology 2019Quote: Each team providing flow cytometry data also obtained their own sample of SpheroTech RCP-30-5A Rainbow Calibration Particles (SpheroTech). A sample of this material is a mixture of particles with eight levels of fluorescence ...
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bioRxiv - Immunology 2021Quote: ... The cells were screened in a 30 min time-course assay for secretion of antibodies that bound to 7 µm streptavidin beads (Spherotech) coated with 10 µg/mL SARS-CoV-2 spike or RBD ...
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bioRxiv - Molecular Biology 2021Quote: ... Gain of the various channels was optimized by analyzing 8 Peak Rainbow Calibration Beads from Spherotech (Cat. RCP-30-5A). Gain was increased incrementally from 25 to 3000 in each channel ...
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened in a 30 min time course assay for secretion of antibodies that bound to streptavidin beads (Spherotech) coated with 10µg/mL biotinylated SARS-CoV-2 S-2P ...
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bioRxiv - Molecular Biology 2021Quote: ... constructs were mixed with 4 μl of polystyrene beads coated with antibodies against digoxigenin (AD beads, 0.1% v/v suspension, Ø 1.76 μm, Spherotech), 10 μl of assay buffer (20 mM HEPES ...
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bioRxiv - Synthetic Biology 2020Quote: ... GFPmut3* fluorescence units were converted into standardized units of molecules of equivalent fluorescein (MEFL) using a fluorescent bead standard (Rainbow calibration standard; cat. no. RCP-30-20A, Spherotech, Inc.) as described previously(Castillo-Hair et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and PMT voltages were adjusted to predetermined target channels using the seventh peak of 8-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL, Cat. No. RCP-30-5A;) as a reference point ...