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Citations for Spherotech :
1 - 38 of 38 citations for Human Endothelin 2 EDN2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... fluorescent microspheres (Spherotech, FP4060-2) were imaged following the same procedures used for biocytin filled cells (Figure S5).
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-coated beads (2 μm diameter, Spherotech) were introduced into the chamber and incubated for 3 minutes to allow the beads to settle on the biotinylated coverslip surface ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µm and 3.4 µm polystyrene beads (Spherotech) were used for size calibration ...
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bioRxiv - Bioengineering 2022Quote: ... which were purchased from Spherotech (Cat. No. FCM-4056-2).
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bioRxiv - Immunology 2023Quote: In-channel IgG capture assays were performed using single assay mixtures containing anti-human IgG-coated 6-8μm beads (Spherotech) and fluorescently-labeled detection anti-IgG antibodies (2.5μg/mL ...
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bioRxiv - Microbiology 2021Quote: To measure shedding of polystyrene beads (Spherotech FP-0552-2, sky blue), flow cytometry was used to quantify the number of egested beads ...
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bioRxiv - Neuroscience 2022Quote: ... of 500 nm diameter fluorescent polystyrene beads (Spherotech, Inc., FICP-08-2) embedded in agarose placed inside a quartz cuvette (Thorlabs ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter 35 ...
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bioRxiv - Neuroscience 2024Quote: ... voltages were setup according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter64 ...
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bioRxiv - Immunology 2021Quote: ... serum and IL-2 starved OT1 CTLs were incubated with streptavidin polystyrene beads (Spherotech) coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Tethers were pulled with a 4 µm diameter polystyrene bead (Spherotech #DIGP-40-2) held at the tip of a micropipette and controlled by micromanipulators ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set up according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter [46] ...
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bioRxiv - Neuroscience 2023Quote: ... Voltages were set up according to optimal PMT sensitive using the peak 2 (Spherotech) voltration technique ...
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bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
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bioRxiv - Immunology 2020Quote: ... Routinely alignments were performed with Ultra Rainbow Fluorescent Particles 3.0 µm (Spherotech, URFP-30-2). The triggering threshold was applied to the side scatter on the 488-SSC channel and the relative applied voltage was determined by using size reference beads (Apogee flow systems ...
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bioRxiv - Cell Biology 2023Quote: ... We then incubated each sample with 0.22-μm light yellow beads (Spherotech, FP-0245-2) resuspended in 2× SSC as fiducial markers for drift correction and washed the sample with 2× SSC briefly.
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bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
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bioRxiv - Microbiology 2021Quote: ... and Nile Red nominally 10 µm (10-14 µm) (Spherotech, FH-10056-2, 107 microspheres/ml) with unmodified surfaces ...
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bioRxiv - Biophysics 2024Quote: ... in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech, Cat. No. DIGP-20-2) in Trap2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
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bioRxiv - Biophysics 2021Quote: 0.5 μm polystyrene fluorescent beads (FP-0545-2; SPHERO Fluorescent Particles, Light Yellow, 0.4-0.6 μm; Spherotech, USA) were injected at the 1-cell stage into the cytoplasm-yolk interface ...
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bioRxiv - Cell Biology 2019Quote: ... was used to capture flow of Sphero™ 2 μm high intensity fluorescent beads (Spherotech, Lake Forest, IL, USA). For association studies ...
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Biophysics 2019Quote: The 8.1 kbp dsDNA construct with a primer-template junction at one terminus was tethered between 2 μm anti-digoxigenin and 3 μm streptavidin functionalized beads (Spherotech) held in place by a micropipette tip and a dual beam optical trap ...
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bioRxiv - Immunology 2020Quote: ... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... ARP-12026) was to a 400 µL volume of fluorescent beads (1.7-2.2-micron SPHERO Carboxyl Fluorescent Particles, Spherotech Inc, CFP-2052-2) using carbodiimide chemistry as described previously (Chu ...
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bioRxiv - Immunology 2024Quote: ... and XBB.1.5 strains of SARS-CoV-2 were incubated with different fluorescence intensity peaks of the SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3-2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...