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Citations for Spherotech :
1 - 31 of 31 citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the raw data were converted to plate-calibrated ERFs using rainbow calibration particles (Spherotech). For a given cell population and readout ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Immunology 2023Quote: ... 1 x 104 counting beads (Spherotech) were added to stained samples at the final step ...
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bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Biophysics 2021Quote: ... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Biochemistry 2019Quote: ... prior to binding of the complex to 1 μM diameter polystyrene beads (Spherotech) functionalized with an anti-digoxigenin antibody (Roche ...
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Biophysics 2021Quote: ... 50 μl of 1% w/v 800-nm streptavidin-coated polystyrene beads (Spherotech Inc) were washed three times in 200 μl 1x PBS buffer and centrifuged at 7500 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... microglia was incubated with ANXV (1:20) for 15’ and fluorescent particles (3um) (Spherotech) were added to microglial cells at a 1:1 microglia/beads ratio for 2hr ...
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (16) ...
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bioRxiv - Microbiology 2020Quote: ... Each cytometer run was calibrated with 1 mm diameter fluorescent Ultra Rainbow beads (Spherotech Inc.). 100 μL for autofluorescent cells and 75 μL for heterotrophic cells were run at an average flow rate of 20 μL min−1 and monitored with a liquid flowmeter (Sensirion US) ...
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bioRxiv - Immunology 2020Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (Su et al. ...
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bioRxiv - Immunology 2024Quote: ... Frequency calculation was obtained by mixing 1/10th of samples with 200,000 fluorescent beads (Spherotech) for normalization.
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Biophysics 2023Quote: ... of ∼0.045 pN nm−1 while using the polystyrene bead of 0.56 μm (streptavidin conjugated, Spherotech).
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
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bioRxiv - Bioengineering 2021Quote: ... One percent (w/v) stock solutions of 1-1.4μm high-intensity polystyrene Nile red fluorescent microparticles were procured from Spherotech Inc (FH-1056-2 ...
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bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
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bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...
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bioRxiv - Biophysics 2022Quote: An aliquot of the crosslinked protein-DNA sample was incubated with 1 μL anti-digoxigenin coated polystyrene beads 2.17 μm in diameter (Spherotech, IL), diluted in 1 mL phosphate-buffered saline (PBS) ...
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bioRxiv - Biophysics 2023Quote: ... Measurements were performed using a dumbbell conformation: the protein of interest was attached in between two 1 μm-sized silica beads (Spherotech, US), using double stranded (ds ...
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bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...