Labshake search
Citations for Covaris :
1 - 40 of 40 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The DNA Hi-C libraries were sheared into 300 bp using a Covaris S220 apparatus (Covaris) and the biotin-labeled fragments were selectively captured by Dynabeads Myone Streptavidin C1 ...
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bioRxiv - Genomics 2023Quote: ... Hi-C template DNA was sheared to a size of 300-500 bp using microTUBE snap-caps (Covaris, 520045) in a Covaris M220 sonicator and MyOne Streptavidin T1 beads (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina, PT0017_Covaris_20ng_Illumina ...
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bioRxiv - Genomics 2020Quote: ... Library preparation followed ultrasonic fragmentation (Covaris: 50 s, 5% duty factor) using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit with an insert size of 350 bp ...
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bioRxiv - Genomics 2021Quote: Chromatin was extracted and sheared from ~120 mg human hippocampus using truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5-25 µg of DNA was sheared using a Covaris g-TUBE (Covaris) and gel size-selected for 1.5 kb ...
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bioRxiv - Immunology 2021Quote: Ten million naïve or LPS-stimulated (0.1mg/mL for 4 hours) human peripheral blood-derived macrophages were cross-linked and processed using truChIP kit (Covaris Inc), as described in detail before(Mehta et al. ...
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bioRxiv - Genetics 2020Quote: Purified human genomic DNA was sheared into ∼150-200 base-pair fragments using the S220 Focused-Ultrasonicator (Covaris, Woburn, Massachusetts) according to the instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent SureSelectXT Human All Exon V6+COSMIC or Agilent Human All Exon 50Mb library preparation protocols were used with inputs of 100-3000ng sheared genomic DNA (Covaris). Library construction was performed using the Agilent Technologies SureSelectXT Reagent Kit ...
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bioRxiv - Genomics 2019Quote: ... Approximately 5 µg of genomic DNA was fragmented with a focused ultrasonicator system (Covaris). Fragmented DNA was used to prepare Illumina paired-end (PE ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples yielding at least 5 ng of cDNA were sheared by sonication (Covaris, S220/E220 focused-ultrasonicator ...
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bioRxiv - Genomics 2022Quote: ... 5-10 mg of snap-frozen tissue was loaded in tissueTUBE-TT05 (Covaris 520071) and cryopulverized using the Covaris CP02 cryoPREP Automated Dry Pulverizer following manufacturer’s procedure ...
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bioRxiv - Genomics 2023Quote: ... Human FFPE genomic DNA from lung tumor tissue was fragmented to 200 bp average size using a Covaris S2 (Covaris Inc.) with the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... input DNA was sheared to an average size of 5-10 kb using gTubes (Covaris) and concentrated using 0.6V Ampure beads (Pacific Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were sonicated and lysed for 5 minutes using the LE220-Plus focused-ultrasonicator (COVARIS) using 350 power peak intensity (PIP) ...
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bioRxiv - Microbiology 2019Quote: ... 2-5 µg of genomic DNA was sheared with a Covaris M220 Focused-ultrasonicator (Covaris) into 400-600bp fragments ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of DNA was sheared to 10 kbp using a g-Tube (Covaris, USA), and a SMRTbell library was made with the SMRTbell template prep kit 1.0 (Pacific Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... approximately 50 µL of gDNA (2.5 – 5 µg) was sheared with a sonicator (Covaris S2) in a 130 µL cuvette with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... 5 ug genomic DNA was sheared to ∼15 kb fragments by a g-TUBE (#520079, Covaris) centrifugation (2,000 Xg ...
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bioRxiv - Developmental Biology 2019Quote: ... in 1× fixing buffer for 5 min at room temperature according to the vendor’s manual (Covaris). Crosslinked cells were then resuspended in 1× shearing buffer and sonicated with Covaris E220 for 780 sec using the following settings ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µg of genomic DNA was fragmented using a Covaris E220 (Covaris, Inc. Woburn, MA, USA) with the following settings ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS ...
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bioRxiv - Microbiology 2022Quote: ... DNA was sheared (plasmid: ∼0.5–2kb; gDNA: 5–10 kb) using g-TUBEs (Covaris, Woburn, Massachusetts, USA) and sheared DNA was used to prepare SMRTbell template sequencing libraries ...
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bioRxiv - Systems Biology 2023Quote: DNA (5 µg) was fragmented by sonication to 200 bp with the M220 Focused-Ultrasonicator (Covaris, USA). End-repair ...
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bioRxiv - Genetics 2019Quote: ... Cells were resuspended in 1 ml shearing buffer (10 mM Tris pH 8.0, 5 mM EDTA, 0.1% SDS) and sonicated (Covaris) with the following settings (Peak Incident Power = 100 W ...
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bioRxiv - Microbiology 2020Quote: 800 ng of genomic DNA isolated from week 2 and week 5 samples was sonicated using an E220 focused ultrasonicator (Covaris) to produce 400 bp fragments which were purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
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bioRxiv - Neuroscience 2023Quote: ... (100 µL per well) and sonicated for 5 min using the Covaris FPPE protocol using the R230 Focused Ultrasonication Device from Covaris (peak power ...
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bioRxiv - Microbiology 2020Quote: ... Extracted chromatin was sheared in SDS lysis buffer (200 μl 1× 109 trophozoites and 5×108 gametocytes) to obtain a fragment size of 100-150bp using an M220 focused-ultrasonicator (Covaris Inc.) using the following settings ...
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bioRxiv - Microbiology 2021Quote: ... DNA was sheared to an average length of approximately 5-10 kb (genomic DNA) using g-TUBEs (Covaris, Woburn, MA, USA) and SMRTbell template sequencing libraries were prepared using sheared DNA ...
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bioRxiv - Microbiology 2023Quote: ... were subjected to soni cation using the Covaris S220 (Power = 140, Duty = 5, Cycles/Burst = 200) for 18 minutes with microTUBE 500 AFA tubes (Covaris 520185) and each sample was incubated with 5 µg anti-FLAG M2 antibody (Millipore A2220 ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was resuspended in water and sheared into 3-5 kb fragments using the S220 Focused-ultrasonicator (Covaris Inc., MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (5-10 µg) samples were sheared to an average size of 20 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing ...
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bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
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bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... protein–DNA cross-links were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The gRNA was fragmented into approximately 200 nt long fragments using an S220 Focused-ultrasonicator (Duty Cycle 10%; Intensity 5; Peak Incident Power 175 Watts; Cycles per Burst 200; Processing time 200 s; Covaris, Woburn, MA, USA). The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: DNA shearing was first performed from 5 µg HMW DNA in 120 µL elution buffer using a g-tube (Covaris Part No. 520079) with 6 passes of centrifugation at 1,990 x g for 2 min ...