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Citations for Covaris :
1 - 48 of 48 citations for 8 Chloro pyrido 3 4 b pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... DNA (3 μg) was sonicated at intensity 4 for 200 cycles per burst for 55 s (Covaris S2), and DNA fragments were end-repaired ...
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bioRxiv - Cancer Biology 2023Quote: ... 10mM hydroxyflutamide for 3 or 16 hours and nuclear fractions were sonicated using a waterbath sonicator at 4°C (Covaris). Supernatants were precleared using Protein G DynaBeads (Thermo ...
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bioRxiv - Genetics 2022Quote: ... 8 kb was done using g-TUBE (Covaris) following manufacturer protocols ...
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bioRxiv - Genomics 2020Quote: ... 8 μg of gDNA was sheared using g-Tubes (Covaris) and concentrated with the AMPure PB magnetic beads.
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bioRxiv - Evolutionary Biology 2023Quote: ... 8°C) and Covaris microTUBE Snap-Cap tubes (Covaris 520045). Sequencing libraries were produced using the KAPA HyperPrep Kit (Roche 07137923001 ...
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bioRxiv - Microbiology 2020Quote: ... then fragmented at 8 kbp with g-TUBEs (Covaris, Woburn, MA) to homogenized DNA fragments lengths increasing accuracy of input DNA molarity calculation to maximize yields ...
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bioRxiv - Genomics 2020Quote: ... 8 kb and 20 kb fragmentation was done using g-TUBE (Covaris) following manufacturer protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was resuspended to 350 μl of 1 M buffer B and sonicated (Covaris S220 sonicator ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science ...
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bioRxiv - Microbiology 2023Quote: ... 8 µg genomic DNA was sheared using g-tubes from Covaris (Woburn, MA, USA) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The epidermis was homogenised in 8 M urea buffer with an ultrasonicator (S220X, Covaris, Brighton, UK) with 175 W peak power for 8 min ...
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bioRxiv - Microbiology 2024Quote: ... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
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bioRxiv - Immunology 2021Quote: ... B cell cultures were harvested at d2 and chromatin extracts were prepared using the truChIP Chromatin Shearing Reagent Kit (Covaris). 100μg of sonicated DNA protein complexes were used for immunoprecipitations with anti-IRF5 (Abcam #ab21689) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-3 million nuclei were sonicated in 130μl microtube by Covaris M220 instrument (Power ...
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bioRxiv - Immunology 2021Quote: ... in immunoprecipitation buffer (0.1% Triton X-100, 0.1 M Tris-HCl pH 8, 0.5 mM EDTA, and 0.15 M NaCl in 1Å~ Covaris D3 buffer) at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Samples were sheared on the Covaris® LE220plus using the 8 microTUBE strip V1 (PM# 520053; Covaris, Woburn, MA) with the base pair (bp ...
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bioRxiv - Bioengineering 2021Quote: ... All samples were stored in low TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8) and were fragmented by Covaris ultrasonicator to have a mean size of 150 bp except cfDNA ...
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bioRxiv - Microbiology 2024Quote: ... DNA was diluted to 3 µg/130 µL in microTUBEs (Covaris #520045) and sheared in triplicate to 300 bp fragments using the following settings on a Covaris LE220 Focused-Ultrasonicator ...
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bioRxiv - Genomics 2019Quote: ... for preparation of 15 kbp libraries 8 µg genomic DNA and 1.4 µg plasmid DNA was sheared using g-tubes™ from Covaris, Woburn ...
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bioRxiv - Plant Biology 2019Quote: ... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
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bioRxiv - Genomics 2019Quote: ... DNA was then resuspended in 10 mM T ris-HCl pH 8 and 5 ug aliquots were sonicated by Covaris S2 to fragment sizes in the range of 450-550 bp ...
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bioRxiv - Microbiology 2022Quote: ... post-RCA DNA was debranched and fragmented to 6-8 kb-sized fragments using the above-mentioned protocol with the modification of the g-TUBE (Covaris) being centrifuged at 13200 rpm (16363 RCF ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA (3 μg) was sheared with S2 Focused-ultrasonicator (Covaris, Wobum, MA, USA) and processed by SureSelectXT Human All Exon V5 (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: 120 μl of DNA sample with around 8-10 μg DNA was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg of 2 min ...
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bioRxiv - Genomics 2020Quote: ... two micrograms of high molecular weight genomic DNA from each sample was sheared using g0-tube to ∼8 Kb (Covaris, Woburn, MA). These sheared gDNA samples were size selected to include 5 Kb-9 Kb fragments with Blue Pippin (Sage Science ...
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bioRxiv - Neuroscience 2020Quote: ... Aggregates were then diluted to a concentration of 100 µg/mL with 1x HBSS buffer followed by three 10s pulses of sonication using a 1/8” probe equipped QS5 Sonicator (Covaris, Woburn, MA). For cell culture experiments ...
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bioRxiv - Genomics 2019Quote: ... and genomic DNA from GM12878 lymphoblast cell line (Coriell Institute) were first sheared to an average fragment size of 8 kb using g-tubes (Covaris Cat. 520079). The fragmented DNA was PCR amplified to generate unmethylated DNA using the first steps of low input ligation kit SQK-LWP001 (ONT) ...
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bioRxiv - Genetics 2022Quote: ... Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Cell suspensions were sonicated for 3 minutes in 1 ml Covaris tubes (Covaris, 520130) using Covaris S220 with the following settings ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was sheared to 3-4kb using Adaptive Focused Acoustics technology (Covaris, Woburn, MA, USA) and cloned into the plasmid vector pIK96 as previously described (Ferris et al ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was sheared to 3 kb using a Covaris LS220 (Covaris Inc., Woburn, MA, USA) or 15 kb (for stationary phase samples and bacteroids isolated from dnf mutant nodules and the matched wild-type A17 sample ...
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bioRxiv - Genetics 2022Quote: ... CA, USA. Three microgram of HMW gDNA was sheared to an average size distribution of ~8 kb using the Covaris g-TUBE (Covaris, MA, U.S.A.) in an Eppendorf 5810 R centrifuge at 5,000 rpm for a total of 2 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 times 30 seconds ON/OFF sonication was carried out at high intensity (Covaris S2 Focus Ultrasonicator) to disrupt the chromatin and release nuclear proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... (4) DNA was sheared to a size of 300-500bp using Covaris LE220 machine (Covaris) at the conditions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA samples (3 µg) were sheared to an average size of 12 kb via G-tube (Covaris) before library preparation ...
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bioRxiv - Plant Biology 2020Quote: ... 3 μg of DNA was sheared to 350 bp average size using an S220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Barcoded ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA samples (3 μg) were sheared to an average size of 10 kb via G-tube (Covaris, Woburn, MA) before library preparation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-3 µg of DNA was sheared to 400 bp or 800 bp using a Covaris LE220 pulse focused sonicator (Covaris) and size selected using a Pippin Prep (Sage Science) ...
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bioRxiv - Plant Biology 2023Quote: ... and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W ...
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bioRxiv - Genomics 2022Quote: ... Chromatin was recovered after a centrifuge step (13,400 rcf, 20 min, 4°C) and was subjected to sonication on a S220 focused-ultrasonicator (Covaris) (180s ON – 30s OFF – 180s ON ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was resuspended in water and sheared into 3-5 kb fragments using the S220 Focused-ultrasonicator (Covaris Inc., MA, USA).
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bioRxiv - Immunology 2021Quote: Ten million naïve or LPS-stimulated (0.1mg/mL for 4 hours) human peripheral blood-derived macrophages were cross-linked and processed using truChIP kit (Covaris Inc), as described in detail before(Mehta et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... of a 100mg/mL stock in DMSO was sonicated into 30uL PBS at 4°C using an S220 Focused-Ultrasonicator (Covaris) with sonication at peak power = 140 ...
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bioRxiv - Genomics 2022Quote: ... the resulting 3C templates were purified using AMPure XP beads (Agencourt) and 4 µg of each template was sheared on a Covaris M220 focused-ultrasonicator (Covaris) to achieve 300 bp DNA fragments ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 cycles per burst for 30 min at 4° C to obtain 100-500 base pair chromatin fragments (Covaris, Woburn, CA). Nuclear sheared lysates were clarified by centrifugation at 15,000 x g for 10 min at 4° C ...
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bioRxiv - Physiology 2021Quote: ... The resuspended chromatin was sonicated into fragments (200 to 500 bps long) at 4 C using an ultrasonic crusher (Covaris, Massachusetts, USA). The sheared chromatin was immunoprecipitated with anti-HA antibodies (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
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bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...