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1 - 3 of 3 citations for pcDNA5 FRT since 2020

• Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

  Markus Höpfler, et al., bioRxiv - Molecular Biology 2023

  Quote: ... HEK T-REx wild type or SCAPER KO cells were seeded in 12-well plates and transfected the next day with pcDNA5/FRT/TO rescue plasmids and a puromycin-resistance conferring plasmid (MXS-CMV-PuroR) using TransIT-293 (Mirus). 24 hours after transfection ...
• Cholesterol modulates the human FPN1 iron export function in plasma membrane liquid-ordered microdomains

  Rim Debbiche, et al., bioRxiv - Biochemistry 2023

  Quote: Flp-In 293 T-Rex cells were transfected with the different pcDNA5-FRT-TO plasmid constructs and the pOG44 vector using the TransIT2020 cationic lipid (Mirus Bio LLC), according to the manufacturer’s instructions (ThemoFisher Scientific) ...
• Evidence linking APOBEC3B genesis and evolution of innate immune antagonism by gamma-herpesvirus ribonucleotide reductases

  Sofia N. Moraes, et al., bioRxiv - Microbiology 2022

  Quote: Approximately 250,000 293T cells were seeded in 6-well plates and transfected the next day with 100 ng pcDNA5/TO-A3-EGFP and 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4 ...