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Citations for Mirus Bio :
1 - 41 of 41 citations for Recombinant Human EFNA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... recombinant replication-incompetent lentiviruses were produced by co-transfecting HEK293T cells using transIT-LT1 (Mirus) with pMD2.G and psPAX2 helper plasmids together with lentiCas9-Blast (Addgene ...
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bioRxiv - Immunology 2022Quote: ... Positive recombinant plasmid was transfected into Sf21 cells using the Flashbac Ultra Baculovirus Expression System (Mirus Bio). Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus encoding POLA1 (or its truncation mutant) were produced using the flashBAC ultra system (Mirus Bio). The baculoviruses encoding PRIM1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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bioRxiv - Immunology 2021Quote: ... The obtained recombinant vector was transfected into CHO-S cells using CHOgro High Yield Expression System (Mirus Bio, USA). The culture supernatant containing the RBD protein was harvested after 10 days ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Vero cells were transfected with the recombinant BAC DNA together with pGS403 encoding Cre recombinase (to excise the BAC cassette) using TransIt-LT1 (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
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bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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bioRxiv - Biochemistry 2021Quote: Human POLA1 (NP_058633.2) and POLA1Δ1-337 was cloned into the pOET1 transfer vector (Mirus Bio). Human PRIM1 (NP_000937.1) ...
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bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
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bioRxiv - Molecular Biology 2022Quote: ... RNAs encoding green fluorescent protein (GFP) were complexed with TransIT (Mirus Bio, Madison, WI) according to the manufacturer’s instructions by diluting in EMEM without serum or antibiotics and incubating for 2-5 minutes ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2019Quote: ... VSV-G pseudotyped lentiviral vectors were produced by transfection of human embryonic kidney cells (HEK293FT) with third-generation lentivirus plasmids using lipofection (Mirus TransIT®-293). Supernatant was collected 48 h after transfection and concentrated using ultrafiltration (Centricon Plus-20 PLGC centrifuge filter units).
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1.5µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167µg of pMD2.G and 1.3µg of p8.91) with TransIT-293 (Mirus Bio). After 2-3 days of transfection ...
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bioRxiv - Microbiology 2021Quote: ... Transient transfections for protein expression in 293T were performed with Transit-LT1 transfection reagent (Mirus Biotech) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... individual wells were transfected with plasmids encoding Venus fused BH3-proteins using TransIT-X2 reagent (Mirus). Non-transfected wells were treated with transfection reagent alone (no DNA added) ...
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bioRxiv - Developmental Biology 2021Quote: ... protein expression (15 ng/well) and target (4.5 ng/well) plasmids using TransIT-2020 Transfection Reagent (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The human AD293 and iPSC cell lines were transfected using the Mirus TransIT®-LT1 Transfection Reagent (Cat # MIR 2300, Mirus Bio LLC, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Constructs were mixed with 0.5μg helper (encoding gag/pol) and 0.1μg encoding coat protein (pMD2) along with 6μL TransIT-Lenti transfection reagent (MIR6603; Mirus Bio; Madison, WI) in 200uL Opti-MEM (31985062 ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcribed full-length CHIKV mRNA and mRNA encoding single viral proteins was transfected into target cells with the TransIT mRNA kit (Mirus). MAYV (strain TRVL15537 ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Systems Biology 2021Quote: ... and grown in complete media for 1 day before co-transfection of the mammalian expression vectors encoding GFP and viral protein of interest using TransIT-2020 transfection reagent (#MIR5404, Mirus). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in RPE1 or HeLa cells for 24–48 h using TransIT®-LT1 Transfection Reagent (Mirus Bio) or Neon Electroporation (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 250 ng of a pCDNA3.1(+) vector encoding for the SARS-CoV-2 spike protein using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Six hours post transfection (p.t.) ...
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bioRxiv - Cell Biology 2022Quote: ... cells in each well were transfected with a plasmid encoding GFP-2A-POI (protein of interest) using TransIT-2020 transfection reagent (Mirus #5404) as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...