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Citations for Mirus Bio :
1 - 44 of 44 citations for Interleukin 10 Receptor Beta IL10RB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 1.0 μg/well of the indicated receptors using TransIT-X2 transfection reagent (Mirus Bio); the total transfected DNA was normalized to 3.0 μg/well for all wells using empty pcDNA3.1 ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviruses for transduction of LTR-mCherry reporter and expression of HIV receptors (CD4, CXCR4, and CCR5) was generated by transfection of HEK293 cells using the Mirus TransIT transfection kit (Mirus). The resulting supernatant was cleared of debris by low-speed centrifugation (300g for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... transfected with 10 μM miR-214-3p using TransIT-X2 (Mirus) for 30 min in a growing medium ...
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivector were transfected into 10 million 293T using TransIT-LT1 reagent (Mirus). Supernatants were harvested at 48 and 72 hrs after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... to a final concentration of 10 μg DNA/well using Mirus TransIT-lnsect (Mirus Bio). 48 h post-transfection ...
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bioRxiv - Genetics 2021Quote: ... plv-sgDiR-EGFP with different guide sequences) were transfected into 10 million 293T using TransIT-LT1 reagent (Mirus), lentivector ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 10 µg of DNA at 70-80% cell density using TransIT-LT1 (Mirus, 2300) for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells seeded in a 10 cm dish were transfected using TransIT-293 (Mirus Bio, Madison, WI, USA) with 670 ng pCAGGS-GP ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% FBS) were transfected using TransIT-X2 or TransIT-LT1 Transfection Reagent (Mirus; using the manufacturer’s protocol) with 250 ng pCMV-VSG ...
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bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 μg mL−1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with DRD1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids containing each of the 10 viral cDNA’s and pCAG FAST P10 plasmid were transfected into BHK-T7 cells using TransIT-LT1 (Mirus)29 ...
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bioRxiv - Microbiology 2021Quote: A549 ZAP−/−/− and DF-1 cells were cultured in 10 cm dish and transfected using LT1 reagent (Mirus lab) with 5 μg of huZAP-GFP DNA for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... APEH (cat# L-005785-00-0010) and control (cat# D-001810-10-05) using TransITX2 (Mirus Bio; cat#MIR6000). UCHL1 knockdown was also carried out by transducing HGSOC cells with control or UCHL1 shRNA lentiviral virus particles (Santacruz Biotechnology ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish and the following day transfected with 10 μg of WT or B.1.617.2 spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Caco-2 cells were plated (10·106 cells) in 500μl of the Ingenio electroporation solution (Mirus Vio, Wisconsin, USA). The transfected cells were selected using the antibiotic geneticin/G418 (Gibco-Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and 100 ug ml-1 Streptomycin were passed to 10 cm dishes and co-transfected using TransIT (Mirus Bio) in an approximate 1:2.5 ratio with NTSR1 containing C-terminal Renilla luciferase (RLuc ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral cell lines were selected with hygromycin (Thermo 10687010, 250 μg/mL) and puromycin (Mirus MIR5940, 10 μg/mL). CT or HLH* EIF3A was cloned into nLV103-hygro ...
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bioRxiv - Cell Biology 2024Quote: ... the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at 10:7.5:5 ratio using TransIT-293 Transfection Reagent (MIR2704, Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were grown in 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: Cells were transfected with the indicated specific short interfering RNA (siRNA) SMARTpools (Dharmacon, Cat# L-004594-00-0005 and Cat# D-001810-10-20) using TransIT-X2 Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... a T25 flask of BHK-21 cells at 75% confluency was transfected with 10 μg of MAYV CMV-promoter driven plasmid using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-293 TREx FlpIn EMX1-EGFP reporter cells were cultured in DMEM supplemented with 10% (v/v) FBS and 50 μg/mL hygromycin (Mirus).
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bioRxiv - Molecular Biology 2023Quote: ... Ligation reaction (10 μL) was mixed with 106 BHK-21 cells in 100 μL of Ingenio® Electroporation Solution (Mirus) and transferred to a 0.2 cm cuvette (Mirus) ...
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bioRxiv - Molecular Biology 2023Quote: The mNG21−10 fragment was a gift from Siyu Feng 47 and was transfected using TransIT-LTI Transfection Reagent (Mirus) and packaged with pNTI673 and pNTI674 (Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... plus 10 µg of the vector of interest using Mirus TransIT-LT1 transfection reagent (Mirus Bio LLC, cat # MIR 2305). Medium was refreshed 16 h post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were transfected with 10 ug of the TRE library ± 5 ug of pcDNA3.1 containing a GPCR expression cassette using TransIT-2020 (Mirus Bio) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
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bioRxiv - Microbiology 2021Quote: ... was diluted to 10 μg/ml and incubated for 60 minutes with a MIRUS Label-IT rhodamine TM reagent (Mirus Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish c and the following day transfected with 10 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in T175 cm2 plates to 85–90% confluency and transfected with the target vectors (10 µg/plate) using TransIT-X2® dynamic delivery transfection reagent (#MIR6006, Mirus). After 48 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7 μg of the vector ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: 5400), or siRNA treatment (50 nM ...
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bioRxiv - Immunology 2024Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7Lμg of the vector ...
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bioRxiv - Genomics 2022Quote: Transection was performed in a 10-cm dish at 70%-80% confluence with 30 ul of TransIT 2020 transfection reagent (Mirus Bio, MIR 5400A) and 10μg of total DNA (1µg of pMT-ROWx2FLAG and 9 µg of bluescript plasmids) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... the renilla luciferase reporter at 0,4 ng/mL and miRNA mimics at 10 nM using TransIT-X2 (Mirus Bio, Madison, WI, USA, #MIR 6000) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... Ad293 cells were cultured in 96 well plates with DMEM containing 10% FB Essence (Avantor Seradigm, USA) and transfected using Transit-LT1 (Mirus Bio, Madison, WI, USA) with plasmid encoding 4 LXR response elements fused with luciferase ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach 50%-70% confluency before transient transfection using TransIT-2020 (Mirus Bio, catalog #: MIR 5400) according to the manufacturer’s instruction.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10 cm dishes and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus Bio, Madison, WI, catalog #: MIR 5400) according to the manufacturer’s instruction ...