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Citations for Mirus Bio :
1 - 18 of 18 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1 Flag-MAVS-C79F or pcDNA3.1-Flag-MAVS-CcardA expressing constructs using TransIT-LT1 transfection reagent (Mirus) before selection using 800µg/ml geneticin (G418 ...
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bioRxiv - Immunology 2021Quote: ... FLAG-p46ATIL or an EV control using TransIT-X2 (Mirus Bio). At 24h post transfection ...
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bioRxiv - Cell Biology 2020Quote: Flag-Astrin constructs were transiently transfected into HEK293T cells using TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... 2.5µg of pCAGGS NA-FLAG (either NAg+ or NAg-) were transfected using 7.5µl of LT1-TransIT transfection reagent (Mirus). Twenty-four hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: ... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
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bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were transfected with 5 μg of the indicated FLAG-tagged TTP wild-type or mutant expression plasmids using TransIT 293 reagent according to the manufacturer’s protocol (Mirus). 48 hours after transfection ...
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bioRxiv - Genetics 2020Quote: ... Constructs containing FP-GRIP1-KBD +/- exon 21 and FLAG-BicD2-KIF5A were expressed by transfection using TransIT-LT1 (Mirus). Approximately 24 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the N-terminal FLAG-tagged CDC42 expression vector using TransIT 293 (Mirus Bio LLC) in 6-well culture plates ...
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bioRxiv - Cell Biology 2022Quote: pQCXIH-SARS-CoV-1-ORF7a-FLAG and pQCXIH-SARS-CoV-2-ORF7a-FLAG were transfected to HEK293T cells with the packaging plasmids pMD.GagPol and pMD.VSVG using Trans-IT293 (Mirus Bio). Viral supernatants were collected 48 hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA6-SARS-CoV-2-ORF3a-FLAG and pcDNA6-SARS-CoV-2-ORF8-FLAG plasmids (kind gifts of Prof Peihui Wang, Shandong University, China) using a commercial liposome method (TransIT-LT1, Mirus). Transfection mixtures containing plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... expression in the indicated cell lines was induced with 1 μg/ml doxycycline and cells were transfected with a pcDNA3-SCAPER-FLAG construct using TransIT293 (Mirus) in 10 cm dishes ...
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bioRxiv - Microbiology 2022Quote: ... were seeded into 6-well plates and transfected the next day with 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). A3B-EGFP or A3A-EGFP expression was induced 6 hours after transfection with 50 ng/mL doxycycline ...
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bioRxiv - Microbiology 2022Quote: Approximately 250,000 293T cells were seeded in 6-well plates and transfected the next day with 100 ng pcDNA5/TO-A3-EGFP and 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips and transiently transfected with mCherry Parkin and PPP3CB-Flag using Trans-IT transfection reagent (Mirus Bio) for 18 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of FLAG-NMNAT1 and FLAG-NMNAT2 constructs (see Supplemental Table 2) was performed at ∼60% confluence using TransIT®-LT1 transfection reagent (Mirus Bio, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 110 ng of anti-CRISPR expression plasmids with 1.25 µl of TransIT-X2 (Mirus Bio) in 20 µl Opti-MEM ...