Labshake search
Citations for Mirus Bio :
51 - 74 of 74 citations for trans Cotinine amide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... and 333 ng of pMD2.G per well) using 3 μL of Trans-IT 293T transfection reagent (Mirus Bio) per μg of DNA ...
-
bioRxiv - Synthetic Biology 2021Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
bioRxiv - Bioengineering 2022Quote: Murine neonatal cardiomyocytes and NHDFs were transfected with miR-146a-5p, miR-126-3p and miR-370-3p mimics (Lightswitch, 100nM final concentration) using Trans-IT TKO (Mirus) in their respective serum free growth media ...
-
bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
-
bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: The CPER products (25 μl out of a 50 μl reaction volume) were transfected into HEK293-3P6C33 cells or BHK-21 cells with Trans IT LT-1 (Mirus), following the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vectors were co-transfected with packaging vectors VSVG and gag/pol in a ratio of 3:1:2 into HEK293T cells using Trans-IT Transfection reagent (Mirus). Viral supernatants were collected at 48 and 72 hr post-transfection ...
-
bioRxiv - Microbiology 2022Quote: ... Control and KO cells were seeded at 40,000 per 48 well and transfected with 200 μg RNA in 25 μL OptiMEM using Trans-IT mRNA Transfection Kit (Mirus) according to manufacturer instructions ...
-
bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...
-
bioRxiv - Microbiology 2024Quote: The CPER products without purification were transfected into Fcwf-4 cells co-cultured with HEK293T cells with Trans IT LT-1 (Mirus) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: 1μg of recombinant Plexin-B1 FL WT pCDNA 3.1 plasmid or Plexin-B1 FL mutants pCDNA 3.1 (as described above) was transfected to 80% confluent COS 7 cells on coverslips using 3 μL Trans IT 2020 transfection reagent (Mirus Bio) for 48 hours at 37 °C ...
-
bioRxiv - Genetics 2022Quote: ... a pooled sgRNA library with LentiCRISPR v1 vector was used to produce lentivirus by transfection of HEK293T (ATCC) using Trans-IT (Mirus Bio) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: ... cells were seeded into six-well plates (Orange Scientific) at a density of 300.000 cells/well and transfected the next day with Trans-IT LT1 (Mirus, WI, USA) and 1:1:1:1 μg DNA encoding hASIC1a TAG variants ...
-
bioRxiv - Microbiology 2022Quote: ... (1.2 μg of each) and C3P3-G1 (0.8 μg) were mixed with Trans-IT LT1 transfection Reagent (12.8 μl) (Mirus Bio, cat. #MIR2305) in OPTI-MEM I Reduced Serum Medium (150 μl ...
-
bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips and transiently transfected with mCherry Parkin and PPP3CB-Flag using Trans-IT transfection reagent (Mirus Bio) for 18 h ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors were produced in HEK-293T cells by co-transfection of the previously sequenced vector constructs with psPAX and pMDG.2 packaging plasmids using Trans-IT LT-1 (Mirus, MIR2700). HEK293T cells were incubated overnight at 37°C and the next day medium was changed for IMDM 10% HI-FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected 24 hours after plating with 1.0 µg D2HGlo constructs and 3.0 µL Trans-IT®-LT1 transfection reagent (Mirus Bio, Madison, WI). The cells were allowed to express for 48 hours before fluorescence imaging.
-
bioRxiv - Genomics 2021Quote: ... and the appropriate pLKO-derived vector (at ratios of 8 µg, 1 µg, and 8 µg, respectively, per 15 cm dish) with Trans-LT1 (Mirus Bio, Madison WI), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: All transfections were performed on HEK 293T/17 cells in the presence of serum-free DMEM and Trans-IT transfection reagent (Mirus Bio, Madison, WI, #MIR 2305). All transductions were performed by spinoculation at 1100 x g for 30 min at 30°C.