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Citations for TriLink BioTechnologies :
1 - 31 of 31 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... T cells were electroporated with 15 ug Cas9 mRNA (TriLink) and 10 ug AAVS1 specific sgRNA using the Neon electroporator (3×105 in 10ul Neon tip ...
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bioRxiv - Microbiology 2021Quote: ... sgRNA (20ng/ul) was microinjected with Cas9 mRNA (50ng/ug purchased from TriLink BioTechnologies) into the cytoplasm of fertilized eggs collected from C57BL/6N mice (Charles River Laboratory) ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Developmental Biology 2023Quote: ... Cas9 (100 ng/µl) (TriLink Biotechnologies), gRNA (50 ng/µl each guide ...
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bioRxiv - Biophysics 2023Quote: ... and 100 µM GpA dinucleotides (TriLink) were added to the solution and incubated for additional 10 min at 37°C to allow the ternary complex to initiat transcription and stall at the first G in the template ...
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bioRxiv - Neuroscience 2022Quote: ... Cas9 mRNA (100 ng/uL. Trilink, L-6125) and both sgRNAs (100 ng/uL each ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 Nickase mRNA (Trilink; 100 ng/μl) in injection buffer ...
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bioRxiv - Genetics 2024Quote: ... and Cas9 Nickase mRNA (Trilink; 100 ng/μl) in injection buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 DL of 100 mM nonradioactive nucleotides [ppGpp (Trilink), ATP ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixes containing 100 ng/µl Cas9 mRNA (5meC,Ψ) (TriLink BioTechnologies), 50 ng/µl sgRNAs and 50 ng/µl ssODN or 50 ng/µl lssDNA were prepared in microinjection buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and CpG 2 µg/ml (TriLink) were added in a final volume of 100 µl ...
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bioRxiv - Molecular Biology 2022Quote: ... dRT (1.5-3 ng/μl) and Cas9 mRNA (100 ng/μl, Trilink Biotechnologies, San Diego, CA) was microinjected into pronuclei and cytoplasm of one-cell stage embryos obtained from superovulated B6D2F1 female mice (Jackson Laboratory ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with the CleanCap® EGFP mRNA (#L-7601-100; TriLink Biotechnologies, San Diego, CA, USA) for the indicated times ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Immunology 2019Quote: ... Mice were generated by injection of a mixture of mammalian optimized Cas9 mRNA (100 ng/μl, TriLink Biotechnologies), purified gRNA_16 and gRNA_23 (50 ng/μl ...
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Bioengineering 2022Quote: ... however 2 μl of the appropriate canonical NTP (75 mM) was replaced by 2 μl of 100 mM 5-Hydroxymethyl-5’-triphosphate (e.g. 5-Hydroxymethyl-CTP) (TriLink).
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bioRxiv - Bioengineering 2023Quote: dAbs were generated by conjugating amine-modified oligonucleotides (15–25 OD260 units) to polyclonal antibodies (100 µg) using the Antibody-Oligonucleotide All-in-One Conjugation Kit from TriLink Biotechnologies (A-9202) ...
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bioRxiv - Molecular Biology 2019Quote: Equal amounts of total RNA (100 ng) were used for small RNA library preparation using the CleanTag small RNA library preparation kit (TriLink Biotechnologies). Adapter-ligated libraries were reverse transcribed and amplified (19 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... sgRNA sequences (20 ng/μL) and its corresponding donor oligonucleotide (100 ng/μL) were co-microinjected with Cas9 nickase mRNA (50 ng/μL, Trilink Biotechnologies) into the cytoplasm of zygotes collected from B6D2F1/J mice (JAX #100006) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions testing IRES nLuc reporters were additionally supplemented with 50 ng/µL (final) competitor FFLuc mRNA (TriLink Biotechnologies # L-7602-100) to increase the fidelity of the IRES nLuc signal ...
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bioRxiv - Genetics 2023Quote: ... The sgRNA (20 ng/ul) and donor oligonucleotides (100 ng/ul) were co-microinjected with Cas9 mRNA (20ng/ul, purchased from Trilink Biotechnologies) into the cytoplasm of zygotes collected from C57BL/6N mice (Charles River Laboratory) ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Systems Biology 2019Quote: eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... transduced activation reporter cells were enriched by puromycin selection (1µg mL-1) following nucleofection of dCas9-VPR mRNA (TriLink) and a Cas9 sgRNA targeting the ESR protospacer (IDT) ...
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bioRxiv - Microbiology 2020Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/ml of cytosine arabinoside (TriLink BioTechnologies), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 104 cells were harvested and incubated in 10 ml Neon Buffer R with 1 ul (1ug/ul) of Clean-Cap Cas9 mRNA (TriLink Biotechnologies) and 1 ul (100 pmol/ul ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared and 12 fractions were collected from polysomes fractionated using the protocol for riboseq gradient preparation above (without nuclease treatment.) 0.3 ml of each gradient fraction was spiked with equal amounts of control RNA (Fluc mRNA, Trilink Biotechnologies, USA), then total RNA was extracted using the hot acid phenol-chloroform method (34) ...