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Citations for Bulldog Bio Inc. :
1 - 35 of 35 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... S1 and S3 were banked using BAMBANKER freezing media (Bulldog Bio, BB02) and kept for downstream analysis by flow cytometry (see section ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were tested for Mycoplasma every 2 months using e-Myco™ Mycoplasma PCR Detection Kit (Bulldog Bio #25235), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were tested for Mycoplasma every 2 months using e-Myco™ Mycoplasma PCR Detection Kit (Bulldog Bio #25235), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... at 2000V using a 2 mm electroporation cuvette (Bulldog Bio) and Eppendorf electroporator and then plated on yeast extract peptone dextrose plus sorbitol plates (YPDS ...
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bioRxiv - Immunology 2021Quote: ... bulk T cells were frozen in Bambanker Cell Freezing Medium at 50e6 cells/ml (Bulldog Bio cat BB01) and stored at −80°C for short term or liquid nitrogen for long term storage ...
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bioRxiv - Microbiology 2021Quote: ... Zoospores were pooled and counted using 2-chip disposable hemocytometers (Bulldog Bio, Portsmouth, NH, USA).
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bioRxiv - Bioengineering 2023Quote: ... T cells were frozen in Bambanker Cell Freezing Media (Bulldog Bio Inc, BB01) and stored in liquid nitrogen until use ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then frozen into aliquots for future use with Bambanker cell freezing medium from Bulldog Bio (#BB01). Post expansion thawed aliquots of T cells were grown at 1e6/mL density in RPMI 1640 media from Thermo Fisher (#11875119 ...
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bioRxiv - Cancer Biology 2021Quote: Early passage cells of all the cell lines listed above were frozen using Bambanker’s freezing media (Bulldog Bio) and were maintained in culture no more than 4 months where early passage vials were thawed ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were concentrated to 1 million cells in 90 μL in an electroporation cuvette (Bulldog Bio; 12358-346) to which 10 μL of ∼1 μg/μL of plasmid DNA was added ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were concentrated to approximately 1 million cells in 90 μL in an electroporation cuvette (Bulldog Bio; 12358-346) to which 10 μL of ∼1 μg/μL of plasmid DNA were added ...
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bioRxiv - Plant Biology 2021Quote: ... and electroporated in a volume of 125 µl in a 2-mm-gap electro cuvette using a NEPA21 square-pulse electroporator (Bulldog Bio), using two poring pulses of 250 and 150 V for 8 msec each ...
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bioRxiv - Physiology 2019Quote: ... 100 μl of resuspended cells and 2-4 μg of desired plasmid DNA in 5 μL of volume were pipetted into electroporation cuvettes (2 mm gap, Bulldog Bio 12358-346) and electroporated using a NEPA21 electroporation system ...
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bioRxiv - Neuroscience 2023Quote: ... The digest reaction product was run in a 2% agarose gel containing RedSafe nucleic acid staining solution (Bulldog Bio Inc, Cat. No. 21141). The gel results were visualized using the Bio-Rad Gel Doc XR+.
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bioRxiv - Neuroscience 2023Quote: ... Cell proliferation was analyzed through live cell counting using phase contrast microscopy within a C-Chip hemocytometer (Bulldog Bio, Portsmouth, NH, USA).
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bioRxiv - Cell Biology 2020Quote: ... and then resuspended in Opti-MEM with 10 μg DNA in an electroporation cuvette with a 2-mm gap (Bulldog Bio, catalog no. 12358346). Cells were electroporated with a poring pulse of 125 V ...
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bioRxiv - Genomics 2024Quote: ... plasmids were transfected into HEK293T cells using GeneCellin (Bulldog Bio) following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The plasmids were transfected into HEK293T cells through GeneCellin (Bulldog Bio) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were regularly tested for mycoplasma contamination (Bulldog Bio, Portsmouth, NH) and fingerprinted using a PowerPlex 1.2 kit (Promega ...
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bioRxiv - Immunology 2019Quote: ... Half of the sorted cells were frozen in Bambanker freezing medium (Bulldog Bio) for ATAC Sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell counts were determined using C-Chip disposable hemocytometers (Bulldog bio, DHC-N01) and equalized across all samples before lysis and RNA extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell mixture was electroporated using a NEPA21 electroporator (Bulldog Bio, Portsmouth, NH) and then transferred into 6-well plates containing complete medium without antibiotics (2 ml) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were tested regularly using the e-Myco PLUS PCR kit (Bulldog Bio #2523348).
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bioRxiv - Microbiology 2022Quote: ... was added to the resuspended cells and electroporated using a NEPA21 electroporator (Bulldog Bio). Electroporated cells were then transferred to a flask containing pre-warmed complete growth medium.
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bioRxiv - Cancer Biology 2023Quote: ... All cells were tested routinely for mycoplasma-free status by PCR kit (Bulldog Bio, 25234).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell lines were also regularly tested for contamination of mycoplasma by e-Myco PCR (Bulldog Bio) and were mycoplasma-free throughout this study.
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bioRxiv - Cell Biology 2020Quote: ... Cells were checked monthly for mycoplasma using the e-Myco plus Mycoplasma PCR Detection Kit (Bulldog Bio 25234).
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bioRxiv - Immunology 2020Quote: ... The cell concentration was then determined using a disposable hemocytometer (C-Chip; Bulldog Bio catalog no. DHC-N01). 3.5 x106 cells were resuspended in 1 ml of PBS ...
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bioRxiv - Biochemistry 2021Quote: ... together with the indicated DNA constructs (0.15 μg total DNA in 25 μl per well) and the cell transfection reagent (1.5 μl GeneCellin (Bulldog Bio) in 25 μl per well ...
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bioRxiv - Cancer Biology 2023Quote: Cell lines were purchased from ATCC and confirmed to be mycoplasma free using the (Bulldog Bio, Cat. No. 2523348). Cells were maintained in RPMI supplemented with 10% fetal bovine serum or 10% dialyzed human serum and cultured at 37°C in 5% CO2 and 95% air ...
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were either used immediately following isolation for electroporation experiments or frozen down in Bambanker freezing medium (Bulldog Bio) per manufacturer’s instructions for later use ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were determined to be mycoplasma free by testing with the e-Myco Plus Mycoplasma PCR Detection Kit (Bulldog Bio, 2523448). NSCLC lines were cultured in RPMI-1640 medium (GIBCO ...
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bioRxiv - Immunology 2023Quote: ... Remaining cells were then pelleted at 400g for 5 minutes at 4°C and resuspended at 1×106 cells/mL in ice-cold BamBanker medium (Bulldog Bio BB02). Cell suspensions were aliquoted at 2.5-5.0×105 cells and frozen directly at −80°C until sorting.
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bioRxiv - Microbiology 2023Quote: ... We confirmed the absence of mycoplasma in all cell lines through monthly testing using an e-Myco PCR detection kit (Bulldog Bio #25234). Cell lines were not authenticated.
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 ml ice cold sorting buffer and cell count and viability was assessed using trypan blue and disposable Neubauer counting chambers (Bulldog Bio, Inc. Portsmouth, NH). Cells were then allocated for direct loading (non-small cell lung cancer and primary uveal melanoma) ...