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Citations for Megazyme :
1 - 30 of 30 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... GlcNAcGlcNAc1A was generated in-house by complete oxidation of N-acetyl-chitobiose (Megazyme; 95% purity) by the Fusarium graminearum chitooligosaccharide oxidase (FgChitO ...
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bioRxiv - Plant Biology 2022Quote: ... Organic nitrogen (Norg) values were calculated by subtracting total N levels by spectrophotometrically determined ammonium (Ammonia assay kit, Megazyme, Bray, Ireland) and nitrate levels (Bloom et al. ...
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bioRxiv - Plant Biology 2023Quote: Carbohydrate digestion assays were performed using either purified barley GBP (heterologously expressed in N. benthamiana) or barley BGLUII (Chandrasekar et al., 2022; available from Megazyme, E-LAMHV). Preparations of the fungal CW and EPS matrix were incubated overnight in sterile MilliQ water at 65°C prior to enzymatic digestion ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of the supernatant containing MLG-derived oligosaccharides was mixed with 10 μL β-Glucosidase (Megazyme) and incubated 15min at 50°C ...
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bioRxiv - Microbiology 2022Quote: ... were inoculated separately with a starting OD600= 0.1 or in different ratios (1:1 and 1:10) in Delft medium + 2% beechwood glucuronoxylan (Megazyme, Ireland) for 120 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... Gln samples in duplicate were prepared by treating 10 µL of extracted AAs and standard curve with 10 µL of 2.7 U of glutaminase (Megazyme, Bray, Ireland) in 40 mM NaAcetate ...
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bioRxiv - Genetics 2021Quote: ... 10 ul of supernatant was added to 100ul glucose assay reagent (Megazyme; K-GLUC) with or without trehalose (1:500 ...
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bioRxiv - Genetics 2019Quote: ... 10 μL supernatant was treated with 0.2 μL water or trehalase (Megazyme, E-TREH) to digest trehalose into glucose or 1 µL amyloglucosidase (Sigma ...
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bioRxiv - Microbiology 2021Quote: Glucose was analysed using the Megazyme GOPOD kit (K-GLUC 10/15, Megazyme Inc., Ireland); ethanol was analysed using the Megazyme LQR kit (K-ETOH LQR ...
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bioRxiv - Microbiology 2023Quote: ... 10 min) and concentrations of lactate in the supernatants were determined using K-LATE kit (Megazyme).
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bioRxiv - Biochemistry 2023Quote: ... 300 μL reactions were set up containing substrate (0.0048-10 mg mL-1 glycogen; 0.0012-1.25 mg mL-1 Pullulan (Megazyme); or 0.0048-1.25 mg mL-1 amylose in a final concentration of 2% DMSO) ...
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bioRxiv - Biochemistry 2023Quote: 5 μL of CVF fluid (not centrifuged) was added to 95 μL of 10 mg mL-1 pullulan (Megazyme) in assay buffer (20 mM sodium acetate ...
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bioRxiv - Zoology 2023Quote: ... Whole-body trehalose levels were measured from 10 μl of supernatant treated with 0.2 μl trehalase (Megazyme; E-TREH) at 37°C for 30 min using glucose assay reagent (Megazyme ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots (10 to 50 µl) were analyzed for sucrose and glucose contents with the K-SUFRG Kit (Megazyme, Bray, Ireland) in triplicate measurements in 96-well plates using a Synergy HTX microplate reader (BioTek ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: L-lactate levels were measured in serum or plasma samples (1:5 diluted in PBS) and in cell culture supernatants (1:10 diluted in PBS) according to manufacturer’s instructions (Megazyme, Wicklow, Ireland). Lactate level in cell culture supernatant were normalized to cell count.
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bioRxiv - Microbiology 2022Quote: Endo-β-1,4-xylanase activity was assayed using a 200 μL mixture of 10 g L−1 beechwood GX (Megazyme, Ireland) in 50 mM sodium acetate buffer (pH 5.5 ...
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bioRxiv - Microbiology 2022Quote: ... and xylan (2% w/v, Megazyme) were prepared with nanopure water and filter sterilized using 0.4 μm surfactant-free cellulose acetate filters (Corning) ...
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bioRxiv - Biochemistry 2022Quote: ... final concentration diluted in 50 mM Tris-HCl buffer (pH 8) and incubated with either VdPelB or AsPel (E-PCLYAN, Megazyme). For each substrate ...
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bioRxiv - Biochemistry 2022Quote: ... final concentration diluted in 50 mM Tris-HCl buffer (pH 8) and incubated with either VdPelB or AsPel (E-PCLYAN, Megazyme). For each substrate ...
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bioRxiv - Genetics 2019Quote: ... 80 μg 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) and 0.0025 μg of purified enzyme ...
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bioRxiv - Plant Biology 2023Quote: ... E-XEGP (xyloglucan-specific endo-β-(1→4)-glucanase (Megazyme, Wicklow, Ireland) by adding 10 μl endoglucanase solution (0.4U/μl ...
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bioRxiv - Biochemistry 2022Quote: ... l-arabino-oligosaccharides (DP = 2-9) obtained commercially (Megazyme) were used as standards at a concentration of 25 μM ...
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bioRxiv - Genetics 2019Quote: The β-mannopyranosidase activity was then assayed using 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) as a substrate according to [66] with the following modification ...
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bioRxiv - Bioengineering 2022Quote: ... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
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bioRxiv - Microbiology 2023Quote: ... in a 24-well plate (6×4) using 100 mg/L of each resin acid: dehydroabietic acid (Megazyme ∼90%), abietic acid (Sigma ∼75%) ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The incubation was carried out at room temperature for 4 hours and followed by adding GlcNAc5 (A5; Megazyme Cat# O-CHI5) to a final concentration of 0.625 mg/ml and continued the incubation at 37 ℃ for 16 hours ...
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bioRxiv - Plant Biology 2021Quote: ... or in vitro reaction products were digested with Neocallimastix patriciarum GH11 enzyme overnight at 30 °C by amending the suspension with 2 μL of enzyme stock (Megazyme). These conditions achieved complete digestion of accessible xylan to xylose ...
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bioRxiv - Biochemistry 2021Quote: ... The mass spectrometer was calibrated with a mixture of manno-oligosaccharides or xylo-oligosaccharides (DP, 2 to 6) obtained from Megazyme. For sample preparation ...
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bioRxiv - Microbiology 2022Quote: ... with a starting OD600= 5 were pipetted onto Delft minimal medium agar plates (2 %) containing 0.4 % beechwood glucuronoxylan (Megazyme, Ireland) or wheat arabinoxylan (Megazyme ...
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bioRxiv - Plant Biology 2020Quote: ... RiSLM and Ecp6 were adjusted to 200 μM and 200 μL of each protein was incubated with 200 μL of 2 mM chitohexaose (Megazyme, Wicklow, Ireland), or 200 μL water as control ...