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Citations for Megazyme :
1 - 49 of 49 citations for 6 1 BROMO ETHYL 4 CHLORO 5 FLUOROPYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... in a 24-well plate (6×4) using 100 mg/L of each resin acid: dehydroabietic acid (Megazyme ∼90%), abietic acid (Sigma ∼75%) ...
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bioRxiv - Immunology 2021Quote: β-1,3(1-6) glucan (Yeast Beta Glucan, Megazyme, Bray, Ireland; 3 mg) was suspended in 4.9 mL MilliQ H2O contaning 100 μL of 1 M NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mg ml-1 linear β-1,2-glucan (Megazyme) in 300 μl final sample volume ...
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bioRxiv - Microbiology 2022Quote: ... mixtures of (GlcNAc)6 (Megazyme) and dialyzed BcLysM1 were incubated for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... E-XEGP (xyloglucan-specific endo-β-(1→4)-glucanase (Megazyme, Wicklow, Ireland) by adding 10 μl endoglucanase solution (0.4U/μl ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cell pellets were resuspended into BMM-C and then inoculated into 5 mL BMM or BMM-xyloglucan media (5 g L-1 xyloglucan, Megazyme) to an initial OD600 of 0.05 ...
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bioRxiv - Plant Biology 2023Quote: ... and laminaripentaose β-1-3-(Glc)5 at a concentration of 1.5 mg·mL−1 were used as standards (Megazyme, Bray, Ireland). To visualize the glucan fragments ...
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bioRxiv - Genetics 2022Quote: ... 3 mM of chitooligosaccharides (DP 3-6) (Megazyme, Wicklow, Ireland) were treated with 0.12 mg/mL of ChitO (Gecco Biotech ...
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bioRxiv - Biochemistry 2023Quote: 5 μL of CVF fluid (not centrifuged) was added to 95 μL of 10 mg mL-1 pullulan (Megazyme) in assay buffer (20 mM sodium acetate ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: L-lactate levels were measured in serum or plasma samples (1:5 diluted in PBS) and in cell culture supernatants (1:10 diluted in PBS) according to manufacturer’s instructions (Megazyme, Wicklow, Ireland). Lactate level in cell culture supernatant were normalized to cell count.
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bioRxiv - Plant Biology 2022Quote: Alcohol-insoluble material from ground seeds or hypocotyls was incubated during 16h at 37°C in 50 mM ammonium formate pH 5 with 5 units of Aspergillus aculeatus endo-polygalacturonase (Megazyme), which hydrolyzed the HG between at least two non-esterified GalA residues and was tolerant to acetyl groups (Voxeur et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of amyloglucosidase (Megazyme, 3260 Units/mL) was added to the reaction mixture containing 10 μL of sample ...
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bioRxiv - Genetics 2019Quote: ... 80 μg 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) and 0.0025 μg of purified enzyme ...
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bioRxiv - Microbiology 2020Quote: ... The increase in absorbance at 340 nm after the supplementary addition of 1.2 U of hexokinase and 0.6 U of glucose-6-phosphate dehydrogenase (Megazyme) allowed us to estimate the amount of glucose units from a standard curve ...
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bioRxiv - Biochemistry 2021Quote: ... The mass spectrometer was calibrated with a mixture of manno-oligosaccharides or xylo-oligosaccharides (DP, 2 to 6) obtained from Megazyme. For sample preparation ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μL of 40 U/mL Aspergillus niger β-glucosidase (Megazyme Ltd.) was added to 100 μL of filtrate ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg/mL peroxidase from horseradish (Boehringer-Mannheim) and 200 nM of one of the tested elicitors: bacterial Ruminococcus flavefaciens glucuronoyl esterase (RfGCE) (Megazyme 9016-18-6), flg22 (AnaSpec AS-62633) ...
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bioRxiv - Synthetic Biology 2020Quote: ... or α-glucosidase (Bottle 5 in the Maltose assay kit; Megazyme Inc., Wicklow, Ireland) (Fig ...
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bioRxiv - Genetics 2019Quote: The β-mannopyranosidase activity was then assayed using 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) as a substrate according to [66] with the following modification ...
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bioRxiv - Bioengineering 2022Quote: ... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
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bioRxiv - Plant Biology 2024Quote: Cellulase digestions were performed by adding 5 U of endo-β-1,4-glucanase (Trichoderma longibrachiatum; Megazyme: E-CELTR) directly to the reaction mixture ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The incubation was carried out at room temperature for 4 hours and followed by adding GlcNAc5 (A5; Megazyme Cat# O-CHI5) to a final concentration of 0.625 mg/ml and continued the incubation at 37 ℃ for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... were inoculated separately with a starting OD600= 0.1 or in different ratios (1:1 and 1:10) in Delft medium + 2% beechwood glucuronoxylan (Megazyme, Ireland) for 120 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... with a starting OD600= 5 were pipetted onto Delft minimal medium agar plates (2 %) containing 0.4 % beechwood glucuronoxylan (Megazyme, Ireland) or wheat arabinoxylan (Megazyme ...
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bioRxiv - Plant Biology 2022Quote: ... The residue was dissolved in 100 µl ddH2O and a 5-40 µl aliquot was used for analysis in duplicates using the D-Glucose Assay Kit (Megazyme International, Ireland) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 300 μL reactions were set up containing substrate (0.0048-10 mg mL-1 glycogen; 0.0012-1.25 mg mL-1 Pullulan (Megazyme); or 0.0048-1.25 mg mL-1 amylose in a final concentration of 2% DMSO) ...
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bioRxiv - Biochemistry 2021Quote: ... 1-4β-D-mannotriose was from Megazyme, Ireland ...
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bioRxiv - Genetics 2021Quote: ... with or without trehalose (1:500; Megazyme; E- TREH) at 37 °C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 unit of Klebsiella planticola pullulanase (Megazyme, Bray, Ireland) for 16 hours at 42°C in 55 mM Sodium Acetate buffer pH 4.5 ...
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bioRxiv - Biochemistry 2023Quote: ... ?-(1-2,3,4,6)-L-fucosidase was obtained from Megazyme (Ayr, UK). Chloroacetamide (CAA) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 U of galactose dehydrogenase (GalDH, Megazyme, Wicklow, Ireland). The absorbance was monitored at 340 nm until equilibrium was reached ...
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bioRxiv - Biochemistry 2022Quote: ... Complex I (1 μg) or Candida boidinii formate dehydrogenase (0.015 U, Megazyme) was incubated with 30 mM of sodium formate for 5 min before starting the reaction with the addition of 200 μM of NAD+ in a total reaction volume of 200 μL ...
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bioRxiv - Microbiology 2023Quote: ... 0.4 U/ml of α-(1–2,3,4,6)-L-Fucosidase (E-FUCHS; Megazyme) was added to the cells and incubated for 3 h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... pH 5.0 (Total Starch hexokinase kit, AOAC Method 996.1 1; Megazyme, Bray, IE). The deep well plate was sealed and secured with a Cap-mat (96-well ...
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bioRxiv - Biochemistry 2020Quote: The hydrolysis of α-maltose-1-phosphate was tested using Aspergillus niger amyloglucosidase from Megazyme (high purity for use in Megazyme Total Starch method ...
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bioRxiv - Biochemistry 2021Quote: ... The counterstain was performed by mixing with 25 ug mL-1 Calcofluor (Megazyme C-CLFR) for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... The first step involved manually adding 75 µl of 1% w/v Beechwood xylan (Megazyme) solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 nM flg22 [u1959ek130 Genescript] or 100 μg mL−1 chitin hexamers(O-ch16 Megazyme) was added to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... phaffii was determined by mixing 1 mg/mL AZCL-HEC substrate (Megazyme, County Wicklow, Bray, Ireland), with 1 mM ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... A standard curve for β-1,3-glucan was constructed by using 0–50 mg ml-1 curdlan (Megazyme) dissolved in 0.1 M NaOH and also heating for 30 min at 50 °C in 1 N NaOH.
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bioRxiv - Plant Biology 2023Quote: ... Samples were digested with 1 U/mg DW sample of Aspergillus aculeatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) (Paterlini et al. ...
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bioRxiv - Plant Biology 2023Quote: 10 mg of dried alcohol insoluble cell wall residues were incubated with 10U.ml-1 of endo-1,4-D-xylanase (E-XYLNP) from MEGAZYME previously desalted with centrifulgal filters (Amicon Ultra-0.5ml ...
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bioRxiv - Microbiology 2020Quote: ... α-D-galactose-1-phosphate (Gal1P) and α-L-fucose (Fuc) were form Carbosynth and xylotetraose was from Megazyme. Galactose (Gal) ...
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bioRxiv - Bioengineering 2021Quote: ... in 100 μL of 0.2 M potassium phosphate buffer (pH 7.0) containing 1 U of endo-1,4-β-Mannanase (Megazyme, E-BMABC). After incubation ...
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bioRxiv - Plant Biology 2021Quote: ... were first subjected to a 2 h digestion in ammonium acetate buffer 100mM pH4 at 40 °C by 2.9 U.mL−1 of Aspergillus acuelatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) to generate OGs which differ in their degrees of polymerization and methylesterification ...
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bioRxiv - Biochemistry 2022Quote: ... Total starch content was determined using a Total Starch HK kit (Total Starch hexokinase kit, AOAC Method 996.1 1; Megazyme, Bray, IE) following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: Endo-β-1,4-xylanase activity was assayed using a 200 μL mixture of 10 g L−1 beechwood GX (Megazyme, Ireland) in 50 mM sodium acetate buffer (pH 5.5 ...
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bioRxiv - Microbiology 2022Quote: ... vitamins and trace metals as described previously (35) additionally containing either 20 g L−1 (w/v) of beechwood glucuronoxylan (Megazyme, Ireland) or 20 g L−1 xylose (w/v) ...
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bioRxiv - Bioengineering 2020Quote: Activity of LsAA9A samples was measured by mixing a reaction solution consisting of 1 mg/mL AZCL-HEC substrate (Megazyme, County Wicklow, Bray, Ireland), 2 mM Ascorbic acid (Sigma-Aldrich ...