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Citations for Biosearch Technologies :
1 - 45 of 45 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Cell Biology 2021Quote: ... single-molecule RNA detection was done using Stellaris FISH probes labeled with Quasar 570 (Biosearch Technologies) and designed against the 5’ end of the M.musculus XBP1 ORF (Supplementary Table 3) ...
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bioRxiv - Systems Biology 2020Quote: Probes for detection of nascent and mature Nanog transcripts were developed using the designer tool from Stellaris (LGC Biosearch Technologies) (Table S1) ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA targets were detected in embryos using smiFISH probes designed to exonic sequence with 5’ end X flap sequences [78] and using secondary detection probes labelled with Quasar 570 or Quasar 670 fluorophore dyes (LGC Biosearch Technologies). Probe sequences are listed in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2024Quote: ... homoeolog-specific KASP (Kompetitive Allele-Specific PCR, LGC Biosearch Technologies) primers were designed using Polymarker (Ramirez-Gonzalez ...
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bioRxiv - Microbiology 2024Quote: ... FailSafe PCR 2X PreMix D or G (LCG, Biosearch Technologies) were used as buffer for all PCR reactions.
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bioRxiv - Bioengineering 2021Quote: ... were immunized for a total of 7 times over 28 days with recombinant ovalbumin (Biosearch Technologies) as a model antigen ...
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bioRxiv - Biochemistry 2023Quote: ... The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies; CL9021K). PCR was carried out using forward primer NI-798 ...
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bioRxiv - Molecular Biology 2020Quote: ... we used the competitive allele-specific PCR (KASP™) assay (LGC, Biosearch Technologies) to genotype the zebrafish.
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bioRxiv - Neuroscience 2021Quote: ... and agrinp168 alleles were genotyped using Kompetitive Allele Specific PCR (KASP, LGC Biosearch Technologies). Animals were staged as previously published (Grandel and Schulte-Merker ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping PCR were done by using KASP genotyping assay (Biosearch Technologies, Hoddesdon, United Kingdom). A similar workflow was used in genotyping adult carrier fish.
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bioRxiv - Neuroscience 2022Quote: ... experimental animals were genotyped using custom Kompetitive Allele Specific PCR (KASP, LGC Biosearch Technologies, UK) genotyping assay [57] ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed as described in Table S1 with 0.25 units EconoTaq enzyme (Biosearch Technologies), 1X EconoTaq Buffer ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Immunology 2021Quote: ... n2019-nCoV (Biosearch technologies, KIT-NCOV-PP1-1000). For subgenomic N-gene quantification ...
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bioRxiv - Molecular Biology 2021Quote: ... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The abundance of SARS-CoV-2 genetic material was subsequently determined by quantitative PCR using a Taqman probe and primers (Biosearch Technologies). The primers and probe sequences for the were as recommended by BEI resources for compatability with synthetic RNA standards (# NR-52358).
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:6 in nuclease free H2O and 5 µl were used for Kompetitive allele specific PCR (KASP) genotyping (LGC Biosearch Technology) (Supplementary Table 3) ...
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bioRxiv - Genetics 2024Quote: The method described here depends on the activity of EZ-Tn5 Transposase (EZ-Tn5 is commonly sold in kits for specific purposes. The enzyme by itself, not part of a kit can be purchased from LGC Biosearch Technologies, Petaluma, CA). This enzyme only requires a 19-nucleotide inverted repeat at each end of a linear blunt-end DNA fragment to catalyze the random insertion of the DNA fragment into a target DNA ...
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bioRxiv - Microbiology 2023Quote: ... RNA was polyadenylated with Poly(A) Polymerase Tailing Kit (BIOSEARCH TECHNOLOGIES). Then the polyadenylated product was reversely transcribed into cDNA using a poly(T ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification kit (LGC Biosearch Technologies) and quantified by NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the MasterPure Gram-Positive DNA purification Kit (Biosearch Technologies) with a modified protocol that included a bead-beating step [53] ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies, Middleton, WI, USA) and quantified on the QIAxpert® spectrophotometer (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted from EVs using the MasterPure Complete DNA and RNA Purification Kit (Biosearch Technologies). For comparison purposes ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenised material was then treated following the Sbeadex® plant mini kit protocol (LGC Biosearch Technologies, Beverley, MA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Fmr1 mutants was reserve transcribed to cDNA using MMLV High-Performance Reverse Transcriptase Kit (Biosearch Technologies #RT80125K) and oligo(dT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was prepared from 0.5 ml of culture with the MasterPure Yeast DNA Purification kit (Biosearch Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Pure lactobacilli isolates had their DNA extracted using MasterPure™ Gram Positive Purification Kit (LGC Biosearch Technologies, Hoddesdon, UK). All DNA extraction quantifications were performed with Qubit Fluorometer (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... ∼5x107 cells were collected and total RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the RNA strands were transcribed in vitro at 37°C using 7.5% (v/v) T7 polymerase from the AmpliScribe T7-Flash transcription kit (ASF3507, Biosearch Technologies), 40mM of Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... All DNA samples for KASP genotyping were extracted from young leaf tissue on an Oktopure DNA extraction system using sbeadex Plant DNA purification kits (Biosearch Technologies). KASP assays were designed and produced by Biosearch Technologies based on provided sequence data for six locations distributed across the region of low recombination on chromosome 5 ...
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bioRxiv - Microbiology 2023Quote: ... Double-stranded oligonucleotides were then mixed in 1:1 ratios and prepared for blunt-end ligation using the End-It DNA End-Repair Kit according to the user manual (Biosearch Technologies). Ligation was performed overnight at 4 °C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA of each isolate was extracted using the MasterPure™ Yeast DNA Purification Kit (LGC Biosearch Technologies, Halle-Zoersei, Belgium), according to the manufactureŕs protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cells at exponential (Fig. 3C) or stationary (Fig. 1C) phase using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Biosearch Technologies), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from samples at the two timepoints of the PCA experiment using MasterPure Yeast DNA Purification Kit (Biosearch Technologies).
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA for sequencing was extracted from exponential phase cultures of UT and UQ isolates using the MasterPure Gram Positive DNA Purification Kit (MGP04100; Biosearch Technologies) or Qiagen DNeasy Powersoil Pro Kit (47016 ...
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bioRxiv - Molecular Biology 2024Quote: A modified DNA extraction protocol was used to produce high molecular weight DNA based on the Lucigen/EpiCentre’s MasterPureTM Complete DNA and RNA Purification Kit A (Biosearch Technologies, Cat MC85200). For HG002 cell line ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...