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Citations for Biosearch Technologies :
1 - 48 of 48 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... NP(6)-BSA-biotin (Biosearch Technologies) was pre-incubated for 30 min with streptavidin-APC in a 1:1 molar ratio ...
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bioRxiv - Immunology 2023Quote: ... 96-well ELISA plates (42592, Costar) were coated with NP23-BSA (Biosearch Technologies) in PBS overnight and washed ...
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bioRxiv - Immunology 2022Quote: ... NP-specific B cells or SA-specific B cells were detected with BCR specific binding with NP-PE (Biosearch Technologies) or SA-PE (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... percentage of NP-binding B1-8hi cell were determined by staining a fraction of cells with 5 mg/mL NP(19)-PE (Biosearch Technologies) and analyzing by flow cytometry.
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bioRxiv - Immunology 2023Quote: Antigen-specific antibodies were captured from immunized mice sera in EIA High Binding surface chemistry 96-well plates (Costar) coated with NP26-BSA (Biosearch technologies) and incubated with dilutions of sera ...
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bioRxiv - Immunology 2023Quote: ... The titers of NP-specific antibody isotypes in sera were measured by ELISA using plates coated with NP(7)- BSA or NP(20)-BSA (Biosearch Technologies) as described previously (63).
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bioRxiv - Cell Biology 2022Quote: Human MYC_intron with Quasar 570 dye (Biosearch Technologies, ISMF-2066-5), human ACTB_intron with Quasar 570 dye (Biosearch Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... human ACTB_intron with Quasar 570 dye (Biosearch Technologies, ISMF-2002-5), Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6 recipient mice were pre-immunized by intraperitoneal injection of 100 µg OVA (BioSearch Technologies) dissolved in PBS and precipitated in alum (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: All oligonucleotides used in this work (Table S2) were synthesized using a MerMade 6 instrument (LGC, Biosearch Technologies) at 1 μmol scale ...
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bioRxiv - Microbiology 2019Quote: ... Re-hydrated samples were hybridized with 62.5 nM of an equimolar mixture of Cy3-labelled DNA probes designed to target the coding region of the gene segment 6 of simian rotavirus A/SA11 (Genbank Acc. AY187029.1) using Stellaris Probe Designer v2 software (LCG Biosearch Technologies), in a total volume of 200 µl of the hybridization buffer (Stellaris RNA FISH hybridization buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous Cdc42 isoforms were detected with 5’ Cy5-labelled Stellaris probes to the exons 6 or 7 (probe sequences available upon request; BioSearch Tech.). Hybridization conditions for Stellaris probes for cultured neurons and for tissue sections was performed as recently described (Terenzio et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:6 in nuclease free H2O and 5 µl were used for Kompetitive allele specific PCR (KASP) genotyping (LGC Biosearch Technology) (Supplementary Table 3) ...
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bioRxiv - Cell Biology 2023Quote: ... the glass coverslips were transferred to a fresh 6-well plate with the cell side up and incubated with Wash Buffer A (Biosearch Technologies) and Hoechst stain (1:2000 ...
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bioRxiv - Microbiology 2019Quote: ... genomic RNA was detected with a 5’ 6-carboxyfluorescein (FAM) and 3’ black hole quencher 1 (BHQ-1) labeled probe targeting nsp2 (Biosearch Technologies, Petaluma, CA), and RNA copy number was calculated by reference to an RNA standard derived from the MHV A fragment ...
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bioRxiv - Cell Biology 2023Quote: Xist RNA was detected with Stellaris® RNA FISH (Human XIST with Quasar® 570 Dye, Biosearch Technologies, SMF-2038-1) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 10 μg/ml trinitrophenyl (TNP)-specific human IgG1-4 (clone 7B4) were incubated with 5 μg/ml TNP-conjugated BSA (TNP-33-BSA, BioSearch Technologies) in PBS for 3 hours gently shaking at 60 rpm ...
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bioRxiv - Genomics 2021Quote: ... human ZBTB16 which were designed targeting the complete coding sequence of human ZBTB16 (GenBank: BC029812.1) using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner (version 4.2) ...
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bioRxiv - Immunology 2021Quote: ... n2019-nCoV (Biosearch technologies, KIT-NCOV-PP1-1000). For subgenomic N-gene quantification ...
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bioRxiv - Genetics 2024Quote: The method described here depends on the activity of EZ-Tn5 Transposase (EZ-Tn5 is commonly sold in kits for specific purposes. The enzyme by itself, not part of a kit can be purchased from LGC Biosearch Technologies, Petaluma, CA). This enzyme only requires a 19-nucleotide inverted repeat at each end of a linear blunt-end DNA fragment to catalyze the random insertion of the DNA fragment into a target DNA ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was polyadenylated with Poly(A) Polymerase Tailing Kit (BIOSEARCH TECHNOLOGIES). Then the polyadenylated product was reversely transcribed into cDNA using a poly(T ...
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bioRxiv - Cancer Biology 2023Quote: ... or 100 ug of the highly substituted hapten NP (NP16 to NP32) conjugated to the carrier protein ovalbumin (OVA) (Biosearch Technologies; N-5051) absorbed to Imject Alum Adjuvant (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification kit (LGC Biosearch Technologies) and quantified by NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the MasterPure Gram-Positive DNA purification Kit (Biosearch Technologies) with a modified protocol that included a bead-beating step [53] ...
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies, Middleton, WI, USA) and quantified on the QIAxpert® spectrophotometer (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted from EVs using the MasterPure Complete DNA and RNA Purification Kit (Biosearch Technologies). For comparison purposes ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenised material was then treated following the Sbeadex® plant mini kit protocol (LGC Biosearch Technologies, Beverley, MA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Fmr1 mutants was reserve transcribed to cDNA using MMLV High-Performance Reverse Transcriptase Kit (Biosearch Technologies #RT80125K) and oligo(dT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was prepared from 0.5 ml of culture with the MasterPure Yeast DNA Purification kit (Biosearch Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Pure lactobacilli isolates had their DNA extracted using MasterPure™ Gram Positive Purification Kit (LGC Biosearch Technologies, Hoddesdon, UK). All DNA extraction quantifications were performed with Qubit Fluorometer (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... ∼5x107 cells were collected and total RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the RNA strands were transcribed in vitro at 37°C using 7.5% (v/v) T7 polymerase from the AmpliScribe T7-Flash transcription kit (ASF3507, Biosearch Technologies), 40mM of Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... All DNA samples for KASP genotyping were extracted from young leaf tissue on an Oktopure DNA extraction system using sbeadex Plant DNA purification kits (Biosearch Technologies). KASP assays were designed and produced by Biosearch Technologies based on provided sequence data for six locations distributed across the region of low recombination on chromosome 5 ...
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bioRxiv - Microbiology 2023Quote: ... Double-stranded oligonucleotides were then mixed in 1:1 ratios and prepared for blunt-end ligation using the End-It DNA End-Repair Kit according to the user manual (Biosearch Technologies). Ligation was performed overnight at 4 °C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA of each isolate was extracted using the MasterPure™ Yeast DNA Purification Kit (LGC Biosearch Technologies, Halle-Zoersei, Belgium), according to the manufactureŕs protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cells at exponential (Fig. 3C) or stationary (Fig. 1C) phase using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Biosearch Technologies), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from samples at the two timepoints of the PCA experiment using MasterPure Yeast DNA Purification Kit (Biosearch Technologies).
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA for sequencing was extracted from exponential phase cultures of UT and UQ isolates using the MasterPure Gram Positive DNA Purification Kit (MGP04100; Biosearch Technologies) or Qiagen DNeasy Powersoil Pro Kit (47016 ...
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bioRxiv - Molecular Biology 2024Quote: A modified DNA extraction protocol was used to produce high molecular weight DNA based on the Lucigen/EpiCentre’s MasterPureTM Complete DNA and RNA Purification Kit A (Biosearch Technologies, Cat MC85200). For HG002 cell line ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...