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Citations for Anaspec :
1 - 44 of 44 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... FITC-labeled Aβ (Anaspec) was prepared per the same protocol.
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bioRxiv - Physiology 2021Quote: Fluorescently labeled peptides were synthesized by Anaspec. The fluorescence polarization was performed as described previously by us9 ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorescein-labeled SIINFEKL was purchased from Anaspec. All other chemicals were purchased from MilliporeSigma (Saint Louis ...
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bioRxiv - Bioengineering 2019Quote: ... HiLyte 488-labeled Aβ (1-42) (HiLyte Aβ) and FAM-labeled scrambled Aβ (1-42) (FAM Scr Aβ) were purchased from AnaSpec (Fremont, CA). All other unspecified reagents were purchased from Sigma Aldrich (St ...
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bioRxiv - Bioengineering 2019Quote: ... HiLyte 488-labeled Aβ (1-42) (HiLyte Aβ) and FAM-labeled scrambled Aβ (1-42) (FAM Scr Aβ) were purchased from AnaSpec (Fremont, CA). All other unspecified reagents were purchased from Sigma Aldrich (St ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescent-labeled Aβ peptides (Anaspec, 60492-01, Fremont, USA) and FSB (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... HiLyte™ Fluor 555-labeled (Cat. AS-60480-01, ANASPEC) were dissolved in DMSO to obtain a 1 mM stock ...
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bioRxiv - Neuroscience 2022Quote: Unlabeled and FAM-labeled synthetic human Aβ1-42 peptides (Anaspec) were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP ...
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bioRxiv - Biophysics 2023Quote: ... and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec) were used at 1-2% of final protein concentration.
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bioRxiv - Neuroscience 2023Quote: ... a fluorescently labeled form of arginine vasopressin (FAM-AVP, Anaspec) was infused in a similar way to the dextrans.
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bioRxiv - Neuroscience 2024Quote: ... HiLyte™ Fluor 647-labeled (Anaspec Inc., Cat#: AS- 64161) was resuspended to a 4µM stock in 50µl 1% Na4OH and 3950µl Corning™ Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Cell Biology 2023Quote: ... and labeled with HiLyte647 (HiLyte Fluor 647 succinimidyl ester; Anaspec, 81256), rhodamine (5-(and-6)-carboxytetramethylrhodamine succinimidyl ester ...
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bioRxiv - Biophysics 2023Quote: ... TDP-43PrLd fluorescently labeled using Hilyte Flour 647 succinimidyl ester (Anaspec) and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec ...
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bioRxiv - Biophysics 2022Quote: ... αS was fluorescently labeled using HiLyte™ Fluor 405 succinimidyl ester (Anaspec). Monomeric αS in 20 mM MES buffer pH 6.0 was incubated with 3 molar excess of dye and incubated at 4 °C for 16 h ...
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bioRxiv - Microbiology 2019Quote: ... and oligosaccharides were labeled with 7-amino-1,3-naphthalenedisulfonic acid (81529, AnaSpec) prior to electrophoresis on 20% acrylamide gels ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (Anaspec, AS-633308) was prepared according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of HiLyte™ Fluor 488-labeled Amyloid-beta (1-42) (Anaspec) prepared by reconstituting 0.1mg in 50 µL of 1% NH4OH ...
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bioRxiv - Plant Biology 2023Quote: ... the sections were reacted with polyclonal anti-GFP antibody (AnaSpec, Fremont, CA, US) diluted 1:500 in 3% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... the sections were reacted with polyclonal anti-GFP antibody (AnaSpec, Fremont, CA, US) diluted 1:500 in 3% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were goat anti-rabbit (Pierce, Cat.No.31462, 1:10000) and goat anti-mouse (AnaSpec, Cat.No.28173, 1:10000). All antibodies were diluted in I-BlockTM protein-based blocking reagent (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant SHSY5Y cells were incubated with varying concentrations of Aβ42 (HiLyte Fluor labeled Aβ42, Anaspec) (50 ...
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bioRxiv - Biochemistry 2020Quote: Stock solutions of HiLyte™ Fluor 488- labeled Human Aβ42 (Aβ, 0.1 mg; AnaSpec, USA) were prepared by dissolving the lyophilized peptide (1% NH4OH ...
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bioRxiv - Biochemistry 2020Quote: Fluorescent (HiLyte™ Fluor 488) labeled Aβ42 was purchased for cell culture assays from Anaspec (sequence ...
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bioRxiv - Physiology 2023Quote: 0.1 mg of lyophilized HiLyte Fluor 488-labeled amyloid-β1-42 (Anaspec AS-60479-01) was dissolved in 50 µL of 1% NH4OH and diluted to 0.5 mg/mL with 1xPBS before aliquoting for storage at −20°C until further use ...
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bioRxiv - Immunology 2023Quote: ... Tetanus Toxoid (TT) was labeled with APC or PE using an AnaTag labeling kit (Anaspec) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: αS and TDP-43PrLD were fluorescently labeled using Hilyte Flour 532/647 succinimidyl ester (Anaspec) and Hilyte Flour 647 ...
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bioRxiv - Biochemistry 2020Quote: ... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent Aβ42 oligomers and fibrils were prepared similarly with HiLyte Fluor 555-labeled Aβ42 (Anaspec, AS-60480-01) and mixed with non-labeled Aβ42 peptides in a 1:2 ratio.
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bioRxiv - Bioengineering 2022Quote: ... and then with tetramethylrhodamine (TAMRA)-labeled Ang II (10-8 mol/L in PBS; AS-61181, AnaSpec, Fremont, CA) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Each peptide is N-acetylated and C-amidated to reduce the effect of charged terminal residues. Fluorescein isothiocyanate (FITC)-labeled Tat peptide (FITC-LC-Tat, a.a. 47-57) was purchased from AnaSpec, Inc ...
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bioRxiv - Immunology 2021Quote: ... and custom Mouse amyloid-beta 42 labeled with HiLyte 488 (SQ-ANCPXXXX) were all purchased from Anaspec (Fremont, CA, USA). ACK lysing buffer (10-548E ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4ºC with the primary antibody: rabbit polyclonal antibody against P2RY12 (1:250, #AS-55043A, AnaSpec Inc.). The secondary antibody was ...
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bioRxiv - Molecular Biology 2019Quote: Lyophilized Aβ1-42 and HiLyte Flour 488-labeled Aβ1-42 (FITC-Aβ) (Abcam, Cambridge, UK and Anaspec Fremont, California, USA respectively) were reconstituted in 1% NH4OH to 2 mg/ml concentration and was further diluted to 1 mg/ml in 1X PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The kinase reaction was initiated by the addition of ATP (10 µM final) and fluorescein labeled Scrtide peptide (AnaSpec, Fremont, CA)(1 µM final) ...
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bioRxiv - Microbiology 2019Quote: ... alongside controls of TSB and purified AIP-I (AnaSpec Inc.; Fremont CA). Samples were analyzed using an Acquity UPLC (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... Phagocytosis assays were done by incubating BV2 cells with 100 µM HiLyte™ Fluor 488-labeled human Aβ1-42 (Anaspec #AS-60479-01) for three hours in the presence or absence of the hybrid protein at 37°C for Aβ uptake and at 4°C for surface binding assay following Prasad and Rao (2018).
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC-MGs were treated for 5 minutes with vehicle (DMSO) or 1µM of fluorescently labeled Aβ (1-40) TAMRA (human Aβ, AnaSpec # AS-60488; (Paolicelli et al., 2017)) prepared in microglia basal media with growth factors ...
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bioRxiv - Immunology 2023Quote: ... Blots were washed in 1XTBST and incubated with peroxidase-conjugated goat anti-rabbit (1:20,000, Anaspec, AS28177) for 4 hr at 4οC ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... beads were washed 3x with lysis buffer and purified P300 enzyme was directly used in combination with the SensoLyte HAT (p300)-Assay-Kit (AnaSpec), according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... was labelled with AnaTag Hilyte™ Fluor 594 and purified truncated PE constructs were labelled with AnaTag Hilyte™ Fluor 488 according to the manufacturer’s instructions (#72048, AnaSpec, Fremont, CA). Cells were seeded onto glass coverslips in 6-well dishes (Thermo Fisher Scientific ...