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Citations for Anaspec :
1 - 45 of 45 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... ACE2-expressing HEK293T and HEK293T control cells were lysed and the activity of the ACE protein was assessed by the SensoLyte 390 ACE2 Activity Assay Kit (AnaSpec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... following the manufacturer’s instructions (SensoLyte p300 Assay kit, SensoLyte PCAF Assay kit, Anaspec) using appropriate dilutions of NDGA and anacardic acid ...
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bioRxiv - Genetics 2020Quote: ... using the SensoLyte Malachite Green Assay kit (AnaSpec) in assay buffer (MgCl2 2 mmol/L ...
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bioRxiv - Biochemistry 2020Quote: ... were studied using a commercially available ACE2 inhibition assay according to the manufacturer’s instructions (SensoLyte® 390 ACE2 Activity Assay Kit *Fluorimetric*, AS-72086, AnaSpec, Fremont CA). Each peptide inhibitor was first tested at 4 concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Malachite green reagent (SensoLyte MG Phosphate Assay Kit [AnaSpec]) was added to each reaction and incubated for 30 min before measuring absorbance at 660 nm ...
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bioRxiv - Microbiology 2020Quote: The SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Fremont, CA) was used according to the manufacturer’s instruction to measure the activity of the enzyme in each supernatant fraction obtained from the osmotic shock procedure (Tris/MgSO4 wash ...
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bioRxiv - Pathology 2022Quote: MMP-12 assay kit was purchased from AnaSpec (Fremont, CA). Frozen liver tissue (50-75 mg ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Neuroscience 2023Quote: ... The SensoLyte Red Glucocerebrosidase (GBA) Activity Assay Kit (AnaSpec, AS-72259) was used to determine enzyme activity in serum and whole tissue lysate samples ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used a SensoLyte® HAT (p300) Assay Kit (Anaspec, Fremont, CA). Assay was conducted on a black walled 96-well petriplate (VWR ...
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bioRxiv - Immunology 2022Quote: Elastin cleavage was examined using a SensoLyte® Green Elastase Assay Kit (AnaSpec). Porcine elastase (AnaSpec ...
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bioRxiv - Microbiology 2023Quote: ... MTase assays with unmodified peptides was carried out using 500ng of peptide (AnaSpec) as substrate and Rv2067c as MTase ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1 μM JAG-1 protein (AnaSpec AS-61298, NC0243900) in a flat bottom 48-well plate (Corning 3548) ...
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bioRxiv - Neuroscience 2019Quote: ... Gelatinase activity was measured using the SensolyteGeneric MMP colorimetric assay kit (Anaspec, Fremont, CA). Samples were run in duplicate and end-point enzymatic activity was analyzed using a glutathione reference standard.
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bioRxiv - Microbiology 2021Quote: ... Recombinant PfQC activities were determined using SensoLyte green glutaminyl cyclase activity assay kit (Anaspec). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Bead surface amines were quantified using a ninhydrin-based assay per the manufacturer’s instructions (Anaspec) with slight modification ...
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bioRxiv - Genetics 2019Quote: ... separate cells were harvested and analyzed using the SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Fluorescent (HiLyte™ Fluor 488) labeled Aβ42 was purchased for cell culture assays from Anaspec (sequence ...
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bioRxiv - Biochemistry 2021Quote: Human ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ADAM10 activity was measured via fluorometric FRET assay following manufacturer’s instructions (AS-72226, Sensolyte 520, Anaspec) and as previously described41 ...
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bioRxiv - Biochemistry 2019Quote: ... MGP 1-64 (the first 64 amino acids of matrix Gla protein, Anaspec), 1 uM ...
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bioRxiv - Microbiology 2023Quote: ... Cathepsin B activity in cytosolic fractions was measured using the SensoLyte 520 Cathepsin B Assay Kit (AnaSpec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Cathepsin D (CtsD) activity was measured using the SensoLyte ® 520 Cathepsin D Assay Kit (AS-72170, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: Activity was determined using the SensoLyte®520 ADAM10 Activity Assay Kit (AS-72226, AnaSpec Inc, Fremont, CA), which is based on the FRET substrate 5‐ FAM/QXL™520 with excitation/emission of 490/520 nm ...
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bioRxiv - Immunology 2021Quote: ... Cathepsin L (CtsL) activity was measured using the SensoLyte ® Rh110 Cathepsin L Assay Kit (AS-72217, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... brain samples were analyzed using the Sensolyte 520 Neprilysin Activity Assay Fluorometric Kit (catalog number AS-72223, AnaSpec) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Additional tissue aliquots were used as the source of ACE2 in a commercially available ACE2 assay (AnaSpec, Fremont, CA). The initial velocities were normalized relative to muscle tissue ...
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bioRxiv - Cell Biology 2022Quote: ... RPRPDDLEI) and a reverse control (IELDDPRPR) that were also used in the TIRFM Microscopy Assay were synthesized by Anaspec.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The in vitro testing in this study was performed using a standard fluorometric furin activity assay (Anaspec: Fremont, CA). This assay uses a fluorometric substrate to measure the enzymatic activity of recombinant human furin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The ThT fluorescence assay was performed using the SensoLyte Thioflavin T β-Amyloid Aggregation Kit (ANASPEC, Fremont, CA, USA). Aβ and ThT solutions at final concentrations of 50 μM and 200 μM ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... beads were washed 3x with lysis buffer and purified P300 enzyme was directly used in combination with the SensoLyte HAT (p300)-Assay-Kit (AnaSpec), according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... samples were analyzed using the Sensolyte 520 Neprilysin Activity Assay Fluorometric Kit (catalog number AS-72223, AnaSpec, Fremont, CA, USA) that employs a 5-FAM/QXL FRET substrate ...
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bioRxiv - Cell Biology 2020Quote: ... The MSC medium was changed every 3 days for 14 days following which Alkaline Phosphatase Assay (Anaspec EGT, Catalog # AS-72146) was used to assess mesenchymal stem cell activity according to manufacturer’s instructions and cell pellets were preserved for further downstream analyses.
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of 25 μL of assay buffer containing substrate (FAM-SAVLQ/SG-QXL®520, AnaSpec, Fremont, CA). The substrate was derived from the cleavage sites on the viral polyproteins of SARS-CoV ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ThT assays were performed using the SensoLyte ® Thioflavin T β-Amyloid (1 – 42) Aggregation Kit (Anaspec, Cat no: AS-72214) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Neuroscience 2021Quote: IDE activity was measure in cortical homogenates posttreatment for1 hour using the SensoLyte IDE Activity Assay Kit (AnaSpec, Inc: Cat. # AS-72231) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent labeling of intermediate curli was performed using a HiLyte Fluor 555 protein labeling kit (Anaspec AS-72045). An aliquot of curli or curli-HiLyte555 was thawed from −80 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Phagocytosis assays were done by incubating BV2 cells with 100 µM HiLyte™ Fluor 488-labeled human Aβ1-42 (Anaspec #AS-60479-01) for three hours in the presence or absence of the hybrid protein at 37°C for Aβ uptake and at 4°C for surface binding assay following Prasad and Rao (2018).
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bioRxiv - Cell Biology 2020Quote: ... The activity of human MMP1 were measured by a SensoLyte® Plus 520 MMP-1 Assay Kit (AnaSpec, San Jose, CA, USA; AS-72012) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Single cells were re-plated onto matrigel treated coverslips at low density in the presence of 10nM PACAP protein (AnaSpec) or 0.01% DMSO (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...