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Citations for Anaspec :
1 - 47 of 47 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 3% bovine serum and HiLyteFluor Texas Red-conjugated streptavidin (AnaSpec, Inc ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies to rabbit anti-Elp1 (1:1500) (Anaspec, #AS_54494)) and rabbit anti-GFP (1:2000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies Rabbit anti-Vangl2 (1:100, Anaspec, AS-55659s, now discontinued) and Mouse anti β-II-Spectrin (1:200 ...
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bioRxiv - Molecular Biology 2019Quote: Primary antibodies used for immunoblotting were as follows: anti-IKAP (Anaspec, cat# 54494), anti-acetylated α-tubulin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFP-tag (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381 ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Neuroscience 2020Quote: ... and primary antibodies (rabbit anti-Iba1, 1:500 Wako #016-26461; rabbit anti-P2y12, 1:500 Anaspec #AS-55043A ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFAP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: 1) rabbit anti-pT217-tau at 1:200 (cat# AS-54968, Anaspec). The immunogen used KLH conjugated with synthetic peptides corresponding to human tau at phosphorylated threonine 217 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4ºC with the primary antibody: rabbit polyclonal antibody against P2RY12 (1:250, #AS-55043A, AnaSpec Inc.). The secondary antibody was ...
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bioRxiv - Immunology 2021Quote: ... slides were incubated in blocking at RT for 1 h before overnight incubation at 4°C with the primary rabbit anti-P2Y12 receptor antibody (1:200, AnaSpec #AS-55043A) and labelling for 1 hour with the secondary antibody AF488 goat anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1: 1:1000, Synaptic Systems 234 009; rabbit anti-P2RY12: 1:500, Anaspec AS-55043A). After primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... ISCs (2,000 cells) and Paneth cells (2000 cells) were then seeded into 30 μl Matrigel containing 1 μM Jagged-1 (AnaSpec, San Jose, CA) and 10 μM Y-27632 ...
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bioRxiv - Biochemistry 2024Quote: Cell Penetrating Peptides were purchased from Anaspec. Tha4 APH (KKLPEVGRSIGQTVKSFQQAAK ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were also co-stained with Hoechst (AnaSpec, Fremont, CA).
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bioRxiv - Immunology 2021Quote: ... cells were pulsed with 1 ng/ml SIINFEKL peptide (AnaSpec) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with Hoechst 33258 (AnaSpec Inc; Freemont, CA) and anti-rabbit or anti-mouse secondary antibody conjugated with Alexa-flour 488 for 1 hour at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and cells were incubated with Hoechst stain (Anaspec, AS-83218) for 20 min at room temperature as nuclear staining ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with 1 µg/mL DAPI (AnaSpec Inc 83210) in BY-2 media for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... differentiated SH-SY5Y cells were incubated with 5 μM TAT-TAMRA (AnaSpec) and 3 μM α-synPFF-AF488 either in the presence or in the absence of 250 μM polyP ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were stimulated by addition of SIINFEKL peptide (AnaSpec, AS-60193-1) for 4 hours and expanded for 3 days prior to an experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were treated with 1 µm non-fluorescent Aβ1-42 (Anaspec #AS-20276) in the presence or absence of the hybrid protein in phenol red-free and serum-free DMEM ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant SHSY5Y cells were incubated with varying concentrations of Aβ42 (HiLyte Fluor labeled Aβ42, Anaspec) (50 ...
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bioRxiv - Molecular Biology 2019Quote: Dead cells in the fascicles were stained with Ethidium Homodimer-1 (2μM, AS 83208, Anaspec) previous to 10% formalin fixation (HT5011 ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... separate cells were harvested and analyzed using the SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Fluorescent (HiLyte™ Fluor 488) labeled Aβ42 was purchased for cell culture assays from Anaspec (sequence ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stimulated in the presence of OVA257-264 (1 µg/ml) (Anaspec), recombinant murine IL-2 200 U/ml (Peprotech ...
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DIET INFLUENCES PERIPHERAL AMYLOID β METABOLISM: A ROLE FOR CIRCULATING INSULIN-LIKE GROWTH FACTOR IbioRxiv - Neuroscience 2020Quote: In vitro: Cells were treated during 15 hours with 500 nM soluble Aβ40-HiLyte Fluor™ 488 (AnaSpec) (68) ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae cells were induced to competence by the addition of synthetic competence stimulatory peptide 1 (CSP-1; Anaspec, Inc.). Markerless deletions and replacements of target genes were constructed using the kanR-rpsL+ (Janus cassette ...
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bioRxiv - Neuroscience 2022Quote: ... the following compounds were applied to the cells in Tyrode’s solution via bath application or a custom-made perfusion system: SST-28 (Anaspec), SST-14 (Anaspec) ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... Cells were washed thrice in PBS and incubated with the fluorescent secondary antibody plus Hoechst stain (Anaspec, AS-83218) at room temperature for 1 h ...
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bioRxiv - Immunology 2023Quote: ... The bone marrow-derived dendritic cells were pulsed with 10µM OTII-peptide (OVA 329-337 peptide; Anaspec # AS-64777) for 2 hours at 37°C ...
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bioRxiv - Genetics 2024Quote: ... cells were expanded for 3 days in cRPMI media supplemented with 10 ng/mL OVA (Anaspec AS-60193-1), 5 ng/mL IL7 and 5 ng/mL IL15 ...
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bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
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bioRxiv - Neuroscience 2019Quote: ... Single cells were re-plated onto matrigel treated coverslips at low density in the presence of 10nM PACAP protein (AnaSpec) or 0.01% DMSO (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Molecular Biology 2020Quote: Both cell lines were incubated with media supplemented with 200nM human Beta-Amyloid (1-42) HiLyte Fluor 555 (AnaSpec, Fremont, CA, USA), with or without thiethylperazine (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of cell resuspension was added to 140 µl YPD media with or without the desired concentration of CRAMP (Anaspec, AS-61305) and incubated for 1 h at 37°C with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were collected in media on 1mL Eppendorf tubes before being grown in matrigel supplemented with Jagged-1 (Anaspec, 1 µM) and cultured in advanced DMEM/F12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...
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bioRxiv - Molecular Biology 2021Quote: ... Phagocytosis assays were done by incubating BV2 cells with 100 µM HiLyte™ Fluor 488-labeled human Aβ1-42 (Anaspec #AS-60479-01) for three hours in the presence or absence of the hybrid protein at 37°C for Aβ uptake and at 4°C for surface binding assay following Prasad and Rao (2018).
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bioRxiv - Immunology 2023Quote: ... Whole spleen cell stimulators from C57BL/6 mice were resuspended in RP10 media at 20×106 cells/mL pulsed with or without 100 nM OVA peptide sourced from AnaSpec (SIINFEKL, AS-60193-1) for 1 h at 37°C with gentle shaking every 15-20 minutes ...