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Citations for Anaspec :
1 - 48 of 48 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Biophysics 2021Quote: ... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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bioRxiv - Biophysics 2019Quote: ... was labeled at the N-terminus with succinimidyl 6-n-7-nitrobenz-2-oxa-1,3-diazol-4-yl amino hexanoate (NBD-X SE; Anaspec, Fremont, CA). Free NBD dye was removed by gel filtration using a PD-10 column (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2019Quote: ... UltraPure grade Thioflavin T (ThT) (AnaSpec, Fremont, CA) was dissolved in deionized water at a concentration of 1 mM then filter-sterilized ...
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bioRxiv - Bioengineering 2019Quote: ... UltraPure grade Thioflavin T (ThT) (AnaSpec, Fremont, CA) was dissolved in deionized water at a concentration of 1 mM then filter-sterilized ...
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bioRxiv - Neuroscience 2023Quote: ... Aggregation was verified by Thioflavin T dye (AnaSpec, AS-88306) staining at 440Em/484Ex (38).
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bioRxiv - Immunology 2021Quote: ... Cathepsin L (CtsL) activity was measured using the SensoLyte ® Rh110 Cathepsin L Assay Kit (AS-72217, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP) was obtained from AnaSpec. Glutaraldehyde was purchased from Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were stimulated by addition of SIINFEKL peptide (AnaSpec, AS-60193-1) for 4 hours and expanded for 3 days prior to an experiment ...
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bioRxiv - Biochemistry 2019Quote: ... S16 conjugated at the N-terminus to HiLite-488 was also synthesized (Anaspec).
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bioRxiv - Biochemistry 2022Quote: N-terminal labeling of KSL-128114 with 5-(and-6)-carboxytetramethylrhodamine (TAMRA, Anaspec Inc.) was performed on resin ...
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bioRxiv - Microbiology 2019Quote: ... and ‘A-E-Dap’ tripeptide (L-Ala-D-Glu-mDap) (AnaSpec), were each tested at 5 μl (2 mM ...
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bioRxiv - Neuroscience 2023Quote: ... AngIIfluo (at a concentration of 3µmol/L, obtained from Anaspec, CA) was infused at a rate of 2.86µl/g/rat and allowed to circulate for a duration of 30 minutes 68 ...
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bioRxiv - Biochemistry 2020Quote: ... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... with MMP-2 (AnaSpec) and Clostridium histolyticum collagenase (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 60 µM of ZHX2 (ASKRKSTTPCMVRTS) or 0.75 µM of RPS25 (PPKDDKKKKDAGKS) N-terminal peptides (Anaspec, Fremont, CA) as substrates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The ThT fluorescence assay was performed using the SensoLyte Thioflavin T β-Amyloid Aggregation Kit (ANASPEC, Fremont, CA, USA). Aβ and ThT solutions at final concentrations of 50 μM and 200 μM ...
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bioRxiv - Immunology 2019Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Molecular Biology 2021Quote: Peptide-pull down was performed with synthetic peptide corresponding to H2A N-terminal (aa 1-23) obtained from AnaSpec. 1μg of peptide was incubated with 1μg of GST-tagged protein overnight on wheel at 4°C in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: Synthetic Aβ42 (>95%, Eurogenentec) and Aβ42 Hilyte™ Fluor 488 (>95%, labelled at the N-terminus) were purchased from Anaspec as lyophilised powder ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg/mL LL-37 (Anaspec), or 250 μg/mL kanamycin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ThT assays were performed using the SensoLyte ® Thioflavin T β-Amyloid (1 – 42) Aggregation Kit (Anaspec, Cat no: AS-72214) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...
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bioRxiv - Biophysics 2020Quote: A super streptavidin biosensor (ForteBio, Menlo Park, USA) was coated with 15 μg/ml monomeric N-terminally biotinylated Aβ42 (AnaSpec, Fremont, USA) by overnight incubation a solution at 5 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
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bioRxiv - Neuroscience 2022Quote: ... or 2 μg/mL fluorescent beta-amyloid (Anaspec). Image masks for fluorescence area and phase were generated using IncuCyte 2020C software.
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Immunology 2021Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated ...
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bioRxiv - Immunology 2020Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated.
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bioRxiv - Bioengineering 2022Quote: ... and then with tetramethylrhodamine (TAMRA)-labeled Ang II (10-8 mol/L in PBS; AS-61181, AnaSpec, Fremont, CA) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: The synthetic peptide Adrenomedullin (ADM) (2 nmol, Anaspec, AS-60447) was incubated with either ddH20 (for control condition ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mg of 2-Cl-Trt resin purchased from Anaspec was transferred to a 10 ml Torviq disposable reaction vessel and swelled for 1 hour in dichloromethane (DCM) ...
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bioRxiv - Cell Biology 2022Quote: ... were starved in SILAC DMEM with no methionine for 30 min and then labelled with L-azidohomoalanine (0.5 mM, AnaSpec, #AS-63669) for 20h ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of cell resuspension was added to 140 µl YPD media with or without the desired concentration of CRAMP (Anaspec, AS-61305) and incubated for 1 h at 37°C with shaking ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μl of 20% Pluronic F-127 dissolved in DMSO (AnaSpec) and diluted in HBSS containing 10mg/ml BSA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (GSHWAVGHLM; 50 µg/mL: 2[Ser]-NMC, produced in AnaSpec; San Jose, CA) overnight at 4°C before use ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017). Confocal images were obtained on a Zeiss LSM 510 confocal microscope at the National Institute of Neurological Disorders and Stroke Light Imaging Facility ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017)) ...
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bioRxiv - Immunology 2020Quote: ... before addition of 1 mL of vesiculation buffer (GPMV buffer plus 25 mM PFA (paraformaldehyde, Electron Microscopy Services) and 2 mM DTT (1,4-dithiothreitol, Anaspec). Cells were incubated for 1 hour at 37°C and GPMVs were collected by removing supernatant from cells.
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bioRxiv - Neuroscience 2022Quote: ... stimulation -iPSC-MGs were treated for 2 h with vehicle (DMSO) or 1µM Aβ (1-40) TAMRA (human Aβ, AnaSpec # AS-60488; (Paolicelli et al., 2017)) prepared in microglia basal media with growth factors ...