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Citations for Anaspec :
1 - 38 of 38 citations for 8 8 dimethylpyrano 2 3 h chromen 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... with MMP-2 (AnaSpec) and Clostridium histolyticum collagenase (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg/mL LL-37 (Anaspec), or 250 μg/mL kanamycin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... and then with tetramethylrhodamine (TAMRA)-labeled Ang II (10-8 mol/L in PBS; AS-61181, AnaSpec, Fremont, CA) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... stimulation -iPSC-MGs were treated for 2 h with vehicle (DMSO) or 1µM Aβ (1-40) TAMRA (human Aβ, AnaSpec # AS-60488; (Paolicelli et al., 2017)) prepared in microglia basal media with growth factors ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... or 2 μg/mL fluorescent beta-amyloid (Anaspec). Image masks for fluorescence area and phase were generated using IncuCyte 2020C software.
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of one of the glycopeptides (Muc5AC-3, Muc5AC-13, Muc5AC-3,13, Cst1.4, and Srs13.2 (Anaspec, Fremont, CA), 5 mM UDP-2-(acetylamino)-4-F-D-galactosamine disodium salt (UDP-GalNAc-F) ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Neuroscience 2023Quote: The synthetic peptide Adrenomedullin (ADM) (2 nmol, Anaspec, AS-60447) was incubated with either ddH20 (for control condition ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mg of 2-Cl-Trt resin purchased from Anaspec was transferred to a 10 ml Torviq disposable reaction vessel and swelled for 1 hour in dichloromethane (DCM) ...
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bioRxiv - Pathology 2021Quote: ... The membrane potential of HEK293 cells was examined by loading the cells for 15 min with one of the two voltage sensitive dyes DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol) (AnaSpec, Fremont, CA, USA) or FluoVolt™ (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... One milligram of Aβ (Anaspec) was solubilized at 1 mg/mL in 1 mL of 50 mM sodium tetraborate ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μl of 20% Pluronic F-127 dissolved in DMSO (AnaSpec) and diluted in HBSS containing 10mg/ml BSA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Immunology 2022Quote: ... Miltenyi Biotech) or BMDCs were stimulated for 3 h using Ova peptide 257–264 (SIINFEKL) (Cat no. AS-60193-5, AnaSpec, Fremont, CA, USA). For measuring antigen cross presentation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (GSHWAVGHLM; 50 µg/mL: 2[Ser]-NMC, produced in AnaSpec; San Jose, CA) overnight at 4°C before use ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Neuroscience 2020Quote: ... One mg of lyophilized human Aβ42 (Anaspec) or scrambled Aβ42 (Anaspec ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017). Confocal images were obtained on a Zeiss LSM 510 confocal microscope at the National Institute of Neurological Disorders and Stroke Light Imaging Facility ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017)) ...
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bioRxiv - Immunology 2020Quote: ... before addition of 1 mL of vesiculation buffer (GPMV buffer plus 25 mM PFA (paraformaldehyde, Electron Microscopy Services) and 2 mM DTT (1,4-dithiothreitol, Anaspec). Cells were incubated for 1 hour at 37°C and GPMVs were collected by removing supernatant from cells.
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Biophysics 2021Quote: ... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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bioRxiv - Biophysics 2019Quote: ... was labeled at the N-terminus with succinimidyl 6-n-7-nitrobenz-2-oxa-1,3-diazol-4-yl amino hexanoate (NBD-X SE; Anaspec, Fremont, CA). Free NBD dye was removed by gel filtration using a PD-10 column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... 3% bovine serum and HiLyteFluor Texas Red-conjugated streptavidin (AnaSpec, Inc ...
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bioRxiv - Bioengineering 2023Quote: ... and then split in two aliquots: one third was pulsed with 50 µM OVA (257-264) peptide (Anaspec AS-60193-1) for 2 h at +37°C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were activated with bone marrow-derived DCs loaded for 1 h with indicated concentrations of N4 or Q4 OVA257-264 peptide versions (Anaspec) as previously described (69) ...
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bioRxiv - Immunology 2021Quote: ... a splenocyte suspension from one OT-I Rag2-/- spleen was stimulated with 10nM Ova257-264 peptide (SIINFEKL, AnaSpec, Cat no. 60193-5-ANA) for 48h and cultured for up to 10 days ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Immunology 2021Quote: ... slides were incubated in blocking at RT for 1 h before overnight incubation at 4°C with the primary rabbit anti-P2Y12 receptor antibody (1:200, AnaSpec #AS-55043A) and labelling for 1 hour with the secondary antibody AF488 goat anti-rabbit ...
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bioRxiv - Biochemistry 2020Quote: ... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... cells were expanded for 3 days in cRPMI media supplemented with 10 ng/mL OVA (Anaspec AS-60193-1), 5 ng/mL IL7 and 5 ng/mL IL15 ...
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bioRxiv - Cell Biology 2020Quote: ... The MSC medium was changed every 3 days for 14 days following which Alkaline Phosphatase Assay (Anaspec EGT, Catalog # AS-72146) was used to assess mesenchymal stem cell activity according to manufacturer’s instructions and cell pellets were preserved for further downstream analyses.
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bioRxiv - Immunology 2021Quote: ... 5×106 lung cells were re-stimulated for 3 hours (37°C, 5% CO2) with 10 nM NP366–374 and PA224-233 peptides from Anaspec (Fremont, California) in the presence of Brefeldin/A from BD Biosciences (Franklin Lakes ...