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Citations for SignaGen Laboratories :
1 - 20 of 20 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 15 μg of Strep-tagged plasmids was combined with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid:transfection reagent ...
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bioRxiv - Biophysics 2021Quote: ... Up to 15 μg of individual Strep-tagged expression constructs (normalized to 15 μg with empty vector as needed) was complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: Cre recombinase was expressed in organoids using Recombinant Adenovirus Ad-Cre-GFP from SignaGen Laboratories (#SL100706), which co-expresses GFP as a marker ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected at 80 % confluence with 1 μg of LRRK2 constructs (and 100 ng of HA-tagged vps35 constructs where indicated) and 3 μl of LipoD293™ Transfection Reagent (SignaGen Laboratories) per well of a 12-well plate overnight ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant AAV constructs were packaged in AAV6 capsids and purified by SignaGen Laboratories (#SL100865, >1013 VG/mL). Production of AAV-CMV-unreg-CUG1 was attempted on three occasions but titers >109 VG/mL could not be obtained ...
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bioRxiv - Immunology 2020Quote: 293T cells were cultured in complete DMEM (10% HI-FBS, 1x Pen/Strep) and then transfected using the LipoD293 Transfection reagent (SignaGen Laboratories, cat. SL100668) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Viral injection of the recombinant adeno-associated virus expressing Cre recombinase under the control of human synapsin promoter (rAAV-hSyn-Cre; from SignaGen Laboratories, Rockville, MD) was done by bulk regional viral injection to the visual cortical area of neonatal Cre dependent Gs-DREADD mice at p1 ...
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in a 1:1 mixture of DNA/Polyjet DNA transfection reagent for 5-18 hours according to the manufacturer’s protocol (SignaGen Laboratories). Expression of plasmid DNA was selected using G418 ...
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bioRxiv - Cell Biology 2023Quote: ... using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668). 48 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng pCMV-IFNL2-genomic or pCMV-IFNL2-cDNA using PolyJet transfection reagent (SignaGen labs) according to the manufacturer’s DNA transfection protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with fluorescent reporter constructs (1 μg total DNA/well, 6 well plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a 2:1 mixture of AAV1-CAG-DIO-Ace2N-4AA-mNeon (Virovek) and AAV2-Synapsin-tdTomato (1.23 × 1012 vg ml−1; SignaGen Laboratories) were injected at the following coordinates ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X 150 mm dish of HEK293 cells were transfected with either FLAG-HA-YTHDC1 or FLAG-ZFC3H1 (1-1233) using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories). 18 to 24 hours post transfection the cell pellets were trypsinized ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... B16-F1 cells were transfected with 1 μg DNA of pCMV3 vector encoding IL-6 or the empty control pCMV3 vector (EV) using PolyJet (SignaGen Laboratories, MD, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using LipoD293™ transfection reagent (SignaGen Laboratories; Cat# SL100668) at a 1:2.5 DNA:Lipo ratio (2.5ug transfer plasmid + 2.5ug psPAX2 + 1.0ug pMD2.G and 15ul lipoD per plate) ...
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bioRxiv - Neuroscience 2020Quote: ... LV-Syn-DIO-GluN1-2A-tdTomato (Vigene Biosciences, 2 × 109 transducing units (TU)/mL) and LV-Syn-DIO-mTagBFP2-2A-GluN2B (SignaGen Laboratories, 1 × 109 TU/mL). For control fluorophores ...