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Citations for SignaGen Laboratories :
1 - 21 of 21 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 gp120 1996 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Viral injection of the recombinant adeno-associated virus expressing Cre recombinase under the control of human synapsin promoter (rAAV-hSyn-Cre; from SignaGen Laboratories, Rockville, MD) was done by bulk regional viral injection to the visual cortical area of neonatal Cre dependent Gs-DREADD mice at p1 ...
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bioRxiv - Immunology 2022Quote: ... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
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bioRxiv - Biochemistry 2024Quote: ... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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bioRxiv - Bioengineering 2023Quote: ... CD123-E51K and CD123-E51T donor rAAV6 virus was purchased from SignaGen Laboratories (Frederick, MD, USA). CCR5-KO donor rAAV6 virus was produced in HEK-293T cells and was purified with AAVpro Purification Kit (TakaRa ...
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bioRxiv - Neuroscience 2020Quote: The lentiviral vector for eGFP under control of the human EF1α-promoter was purchased from SignaGen Laboratories (Gaithersburg MD, USA). All other lentiviral vectors were made in the department of Prof ...
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bioRxiv - Microbiology 2024Quote: ... 5 ×105 cells seeded in a 6-well plate were transfected with 40 nM SMARTpool human siRNAs (Horizon) or non-targeting (siNT) control siRNA (Horizon) using LipoJet In Vitro Transfection Kit (Ver. II) (SignaGen Laboratories). Twenty-four hours after the first transfection ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells at 0.25 × 106 were plated into 6-well plates 24 h before the first siRNA transfection with 40 nM of the ON-TARGETplus human siRNA SMARTpool (Horizon Discovery Biosciences) using LipoJet (SignaGen Laboratories, Frederick, MD) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg of a gel-purified mutagenic primer targeting mouse rs11031006 (5’-CTGGAATTTAATATTGCTCTGCCCTGTGATATTTATTTCAAGGTTAGTAGAAATGTAGCTACCTCCTGTAATGACAAATGA-3’) using PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories). At 18 hours post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in a 1:1 mixture of DNA/Polyjet DNA transfection reagent for 5-18 hours according to the manufacturer’s protocol (SignaGen Laboratories). Expression of plasmid DNA was selected using G418 ...
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bioRxiv - Cell Biology 2023Quote: ... using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668). 48 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng pCMV-IFNL2-genomic or pCMV-IFNL2-cDNA using PolyJet transfection reagent (SignaGen labs) according to the manufacturer’s DNA transfection protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with fluorescent reporter constructs (1 μg total DNA/well, 6 well plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a 2:1 mixture of AAV1-CAG-DIO-Ace2N-4AA-mNeon (Virovek) and AAV2-Synapsin-tdTomato (1.23 × 1012 vg ml−1; SignaGen Laboratories) were injected at the following coordinates ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X 150 mm dish of HEK293 cells were transfected with either FLAG-HA-YTHDC1 or FLAG-ZFC3H1 (1-1233) using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories). 18 to 24 hours post transfection the cell pellets were trypsinized ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... B16-F1 cells were transfected with 1 μg DNA of pCMV3 vector encoding IL-6 or the empty control pCMV3 vector (EV) using PolyJet (SignaGen Laboratories, MD, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using LipoD293™ transfection reagent (SignaGen Laboratories; Cat# SL100668) at a 1:2.5 DNA:Lipo ratio (2.5ug transfer plasmid + 2.5ug psPAX2 + 1.0ug pMD2.G and 15ul lipoD per plate) ...
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bioRxiv - Neuroscience 2020Quote: ... LV-Syn-DIO-GluN1-2A-tdTomato (Vigene Biosciences, 2 × 109 transducing units (TU)/mL) and LV-Syn-DIO-mTagBFP2-2A-GluN2B (SignaGen Laboratories, 1 × 109 TU/mL). For control fluorophores ...