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Citations for PacBio :
1 - 34 of 34 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Libraries were built following the “Procedure & Checklist – 2 kb Template Preparation and Sequencing” protocol (PN 001-143-835-08) from PacBio with a few modifications ...
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bioRxiv - Biochemistry 2020Quote: ... Libraries were built as per the “Procedure & Checklist – 2 kb Template Preparation and Sequencing” protocol (PN 001-143-835-08) from PacBio with some modifications ...
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bioRxiv - Genomics 2023Quote: ... barcoded universal primers (barcoded universal F/R primers plate 96 v2, PacBio) were attached to the forward and reverse universal sequences in the amplicons from the first-round L-PCR ...
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bioRxiv - Microbiology 2019Quote: ... a second round of PCR was done with distinct barcoded universal F/R primers as provided by the manufacturer (PacBio, Menlo Park, CA). SMRTbell Library preparation and SMRT sequencing on a PacBio Sequel System was also done according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: We collected three sequence data sets for this study: long-read Pacific Bioscience (PacBio), short-read Illumina whole-genome (DNA-seq) ...
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bioRxiv - Molecular Biology 2020Quote: ... F/R Primers Plate-96v2 (Pacbio part no. 101-629-100).
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 1 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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bioRxiv - Genomics 2023Quote: ... size selection and removal of excess primers was conducted using AMPure PB Beads (PacBio).
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bioRxiv - Genomics 2020Quote: ... SMRTbell library was annealed to sequencing primer at values predetermined by the Binding Calculator (PacBio) and a complex made with the DNA polymerase (P6/C4 chemistry) ...
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bioRxiv - Genomics 2019Quote: ... This ultimately led to the identification of secondary building blocks (see example of distinct L-T-P and L-T-U blocks detected on a set of PacBio reads).
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bioRxiv - Microbiology 2019Quote: ... A new removePrimers function was added to the DADA2 R package that detects and removes primers from PacBio CCS reads ...
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bioRxiv - Genomics 2020Quote: ... Sequencing primer annealing and polymerase binding to the PacBio SMRTbell Templates were performed using the manufacturer’s recommendations (PacBio, US).Library complex wasthen sequenced using SMRT Cell 8M (PacBio)compatible with the Sequel II sequencer.
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bioRxiv - Evolutionary Biology 2021Quote: ... In the first round we amplified the GFP coding region with primers carrying a “universal” sequence provided by PacBio. We amplified the GFP gene from the plasmid libraries obtained after every round of selection (see Selection of transformed cells using FACS) ...
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bioRxiv - Genomics 2022Quote: ... The selected library fractions were bound with Sequencing Primer v2 and Sequel II Polymerase v2.0 and sequenced on Sequel II instruments (PacBio) on SMRT Cells 8M using Sequencing Plate v2.0 ...
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bioRxiv - Genomics 2022Quote: ... The selected library fractions were bound with Sequencing Primer v2 and Sequel II Polymerase v2.0 and sequenced on Sequel II instruments (PacBio) on SMRT Cells 8M using Sequencing Plate v2.0 ...
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bioRxiv - Immunology 2024Quote: ... the 16S rRNA gene amplicons were generated with the barcoded Full-Length 16S rRNA gene primers (forward: AGRGTTYGATYMTGGCTCAG, reverse: RGYTACCTTGTTACGACTT) from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using AMPure®PB beads (PacBio), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR product pools were purified using AMPure®PB beads (PacBio). Sequencing was performed at the Functional Genomic Center Zürich ...
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bioRxiv - Bioengineering 2023Quote: ... 2 weeks later and subjected to large deletions detection (PCR and PacBio sequencing). Single cells were isolated from bulk edited cells into 96-well plates 2 weeks after electroporation and genotyped 2 weeks after isolation ...
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bioRxiv - Microbiology 2021Quote: ... PCR chemicals and conditions (27 cycles) were performed as suggested by Pacific Biosciences (PacBio, 2020). PCR products were visualized on an 1 % (w/v ...
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bioRxiv - Microbiology 2021Quote: The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Bioengineering 2021Quote: ... The genomic region containing integrated BiTE sequences amplified by PCR were gel purified and analyzed by long read amplicon sequencing (PacBio SMRT®). The circular consensus reads (∼3 million CCS reads ...