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Citations for Promega :
1 - 30 of 30 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... After removing the residual genomic DNA with the DNase I Kit (Promega, M6101), 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa ...
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bioRxiv - Synthetic Biology 2024Quote: 1ml of nickel (Ni)-coated beads (Promega, cat. V8821) were transferred into 50ml conical tubes and were allowed to settle ...
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bioRxiv - Biochemistry 2022Quote: ... A similar process was used for Nano-Glo HiBiT blotting (Promega, Madison, WI) wherein samples were prepared for SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Systems Biology 2022Quote: ... 48 and 72h post-plating Cell Titer Glo 2.0 solution (Promega) was added on top of the cell media and incubated for 10min ...
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bioRxiv - Biochemistry 2021Quote: ... Inc and Fugene transfection agent was purchased from Promega. Phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transfections were done using Fugene HD transfection agent (Promega) at ratio 1:4 (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids was done 24 hours after plating using FuGENE6 (Promega) at a DNA/FuGENE ratio of 1:4.5 according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... All transfections were performed with FuGENE 6 transfection agent (Promega E2691) in Opti- MEM Reduced Serum Medium (Gibco 31985070 ...
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bioRxiv - Biochemistry 2023Quote: ... We employed a two-step digestion process (involving lysyl endopeptidase, from FUJIFILM Wako Chemicals U.S.A. Corporation, followed by trypsin, Promega) in-solution to produce the crosslinked peptide pairs.
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bioRxiv - Cell Biology 2020Quote: ... the cells were transfected 16 h after plating using FuGENE® 6 Transfection Reagent (Promega). For PLD2 inhibition ...
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bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).
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bioRxiv - Biophysics 2024Quote: ... The transfection was terminated 24 h later by removing the media and lysing the cells using 25 µl 1x Passive Lysis Buffer (Promega). For eIF1/eIF5 knockdown experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation was evaluated 7 days post-plating using CellTiter-Glo® Luminescent Assay (Promega, #G7570) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Samples representing different stages of the parasitizing process (i.e. root development) were extracted using the Maxwell® RSC Plant RNA kit (Promega) with the Maxwell® RSC Instrument (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
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bioRxiv - Neuroscience 2022Quote: ... purified by a phenol chloroform process and transcribed in vitro into RNA using the T7 RiboMaxTM Large Scale RNA Production System (#P1300, Promega) and the m7G(5’)ppp(5’)G RNA Cap Structure Analog (#S1411 ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in the infected cells was measured by removing the culture supernatant and adding 50 μL Luciferase assay reagent (Firefly luciferase reagent, Promega. Madison, WI). Luminescence was recorded using the luminescence plate reader (Clariostar plate reader ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from 10 flies for each experiment condition and the process followed the protocol of Promega ReliaPrepTM RNA Tissue Miniprep System kit (Promega, Z6110). Extracted RNA was stored in a −70°C refrigerator ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 hours after plating cells were transfected with 1μg of histone H3.3-HaloTag®Fusion Vector DNA (Promega) + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were plated into a 6-well plate and transfected 24 h post-plating with 400 ng pcDNA3.1-HA-RBM39 and 800 ng pcDNA3.1-DCAF15 expression vectors using FuGENE HD (Promega). 48 h post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... the day after plating (3.45 × 104 cells/well in a 24-well plate) using FuGENE HD (Promega, E2312). After 48-h incubation ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Cell Biology 2020Quote: ... The remainder of each extract was added to 40 μl magnetic nickel beads (MagneHis™ Ni-Particles, Promega). Beads were rotated for 2 h at room temperature to purify ubiquitinated proteins and washed once with lysis buffer B supplemented with 20 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The solubilized membrane proteins were subsequently supplemented with imidazole to a final concentration of 50 mM and incubated with nickel-immobilized HisLink resins (Promega) that had been pre-calibrated in Buffer B (100 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured 24 h after plating (T0) and after 4 d using the CellTiter-Glo Luminescent Cell Viability Assay protocol (Promega; cat# G7573). When indicated ...
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bioRxiv - Immunology 2024Quote: ... and 1μg of plasmid expressing the respective spike protein using a 3:1 ratio of Fugene HD transfection agent (Promega).28,49 After 48 hours of incubation at 10% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: MTS growth assays were performed on adherent cells by plating 1000-5000 cells per well in a 96-well plate and assaying using CellTiter 96® AQueous One Solution (Promega, Madison, WI) and incubating for 2 hours and reading at 490nm ...
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bioRxiv - Systems Biology 2021Quote: The effects of inhibitors as single agent on viability of PBMCs was measured using CellTiter-Glo assay (Promega, WI, USA) as described previously3 ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).