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Citations for Promega :
1 - 45 of 45 citations for Fmoc Rink Amide OctaGel Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Resin was then washed 4 times with Resin Wash Buffer (Promega). Complexed proteins were eluted in SDS Elution Buffer (Promega ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...
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bioRxiv - Microbiology 2022Quote: ... or HaloLink resin (Promega). The anti-FLAG beads were washed three times with wash buffer (50 mM Tris [pH 7.4] ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... or HaloLink Resin (Promega) for 1-2 h (20 µl of packed resin/ 1 mg of lysate) ...
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bioRxiv - Biochemistry 2022Quote: 200 μl packed HaloLink resin (Promega) was washed three times in HALO-binding buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: 400 µL of HaloLinkTM resin (Promega, G1912) was washed three times with Halo binding buffer (1x PBS with 0.005% IGEPAL® CA-630 and 0.5mM TCEP) ...
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bioRxiv - Cell Biology 2024Quote: ... Halo-MultiDsk was conjugated to HaloLink Resin (Promega) prior to IP for 1 h at RT in wash buffer (100 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: The clarified lysate was incubated with HaloLink Resin (Promega) for 15min at 22°C with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... were pulled down using HaloLink resin (Promega Madison, WI) and extensively washed in buffer containing 100 mM Tris (pH 7.6) ...
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bioRxiv - Neuroscience 2021Quote: ... purified with phenol:chloroform:isoamyl alcohol (PCI) and Wizard resin (Promega), digested with XbaI and BamHI-HF in Cutsmart Buffer (NEB) ...
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bioRxiv - Genomics 2020Quote: ... 2 mL of Wizard DNA Clean-Up resin (Promega) was added to the solution and mixed by inversion for two minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was incubated with HaloLink Resin (Promega) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Proteins retained in the resin were digested with trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Synthetic Biology 2023Quote: ... HaloTagged GFP (pHM150) was coupled onto HaloLink™ resin (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was incubated with HaloLink resin (Promega, Cat# G1914) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and digested on resin using 1 μg of Trypsin Gold (Promega) at 37°C overnight with agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Microbiology 2021Quote: Phage DNA was extracted using the Wizard resin kit (Promega, Madison, USA) and eluted in TE buffer (after (31)) ...
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bioRxiv - Biophysics 2022Quote: ... Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821 ...
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bioRxiv - Molecular Biology 2024Quote: ... the resin crosslinked with Halo-Nwd1 was incubated with HaloTEV protease (Promega) overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... SIRT5 WT and variants were purified by Ni2+-affinity resin (#V8823, Promega), and eluted with 25 mM Tris/HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... HALO-MultiDsk (2 mg) was incubated with 200 µl of HaloLink resin (Promega) in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Bound proteins were digested on-resin with trypsin (Trypsin Gold, Mass spectrometry grade, Promega) at 37 °C overnight in 25 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was diluted in TBS and incubated with pre-equilibrated HaloLink Resin (Promega) at room temperature with rotation for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 10 nmol of HALO-tagged UBDs were immobilized on 100 μl HALOLink resin (Promega) in 500 μl HALO-coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The preparation of Halo-tagged NEMO and its covalent attachment to Halo-Link Resin (Promega) have been described (Emmerich et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega, G1915) suspension overnight in 4 °C on a rotator ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was incubated with 2.5 ml HaloLink resin slurry (Promega, pre-equilibrated in Halo buffer) and allowed to incubate on a roller for 90 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions from the Ni-NTA eluate were then pooled and passed over SoftLink Avidin resin (Promega). The SoftLink Avidin resin was then washed extensively with SoftLink Avidin Wash Buffer A (20 mM Tris HCl ...
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bioRxiv - Plant Biology 2024Quote: 10 nmol of Halo-tagged MpCESAR CUE domain was incubated with 100 μl of the HaloLink resin (Promega) in 500 μl of the coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Ubiquitin enrichment was performed using Halo-tagged MultiDsk TUBE (Wilson et al., 2012) preincubated with HaloLinkTM resin (G1913, Promega) for 1 hour with rotation at RT in binding buffer (100mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The solubilized membrane proteins were subsequently supplemented with imidazole to a final concentration of 50 mM and incubated with nickel-immobilized HisLink resins (Promega) that had been pre-calibrated in Buffer B (100 mM HEPES pH 7.5 ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... MBP-LIC1 (50 pmol) and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281 ...
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bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from 1 ml concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [74] ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 350 µL of this aqueous phase underwent automated extraction and cleaning using a Tecan Freedom EVO liquid handler with Promega Purefood protocol and reagents (cell lysis buffer, ReliaPrep™ resin, prepared wash buffer, isopropanol, 50% ethanol and Promega elution buffer). The total extracted and washed DNA was eluted in a final volume of 150 µL.
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...