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Citations for Promega :
1201 - 1250 of 4618 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... with m7G(5')ppp(5')G RNA Cap Structure Analog (ref. S1404L, Promega) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Biophysics 2021Quote: ... IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 1% paraformaldehyde/PBS for 1 minute and washed with PBS and with 2X SSC (Promega, V4261), followed by Incubation in 50%formamide/2X SSC (pH7-7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were washed with Wash Buffer three more times and nuclei pellets were resuspended in Diluted Nuclei Buffer (1x Nuclei Buffer, 10x genomicsTM, supplemented with 1 mM DTT and 1 U/uL RnaseIn, Promega©) for counting and scMulti library preparation ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Immunology 2020Quote: Caspase-1 and Caspase 3/7 activities were measured using Caspase-Glo® 1 and Caspase-Glo® 3/7 assay (Promega) respectively ...
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bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated antibody (1:2000; Promega, Madison, WI, USA) and washed again with TBS-T ...
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bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding different Ocrl1 mutants were transfected using Fugene 6 reagent (Promega) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: DNA was transiently transfected into cells using FuGENE 6 transfection reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1,3 μg of plasmid DNA and 3 μl of FuGene 6 (Promega) were mixed in 100 µl Opti-MEMTM medium before the addition to the dish ...
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bioRxiv - Microbiology 2022Quote: All ectopic transfections were performed using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5-6ng pcDNA3.1 FLAG-cGAS and STING using 0.75μl FuGENE 6 (Promega) and 20μl Opti-MEM (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 1.2 µg p8.91 and 1.2 µg KAP1 pHRSIN using FuGENE 6 (Promega). Virus-containing supernatant was collected 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... Viral stocks were generated by transfecting 293T cells using Fugene 6 (Promega). After 48h ...
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bioRxiv - Microbiology 2022Quote: ... The DNA plasmid mix was added to 18µl of Fugene-6 (Promega) transfection reagent in 200µl of pre-warmed Opti-MEM (Gibco ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... Transfections and co-transfections were performed using FuGENE 6 transfection reagent (Promega). Cells were analysed two days post-transfection.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Fugene 6 reagent (Promega) by following the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: RSV VLPs were produced by FuGENE® 6 Transfection Reagent (Promega, E2691) transfection of DF1s at 50% confluence with 1 µg of viral plasmid ...