Labshake search
Citations for Promega :
851 - 900 of 1829 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2020Quote: ... RNA (2 µg) was reverse transcribed through the GoScript™ Reverse Transcription System performed with Oligo(dT)15 (Promega, Madison, Wisconsin, EUA), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... OXA-111-NcoI F or OXA-71-NcoI F and OXA-66-XhoI R primers (Table 2) and TA cloned into the vector pGEM-T Easy (Promega, United Kingdom). The inserts were confirmed by sequencing with the universal T7 Promoter primer ...
-
bioRxiv - Immunology 2021Quote: ... Luminescence was read after 2 minutes shaking on an orbital shaker and 10 min incubation at RT using the GloMax instrument (Promega, Madison, USA).
-
bioRxiv - Immunology 2021Quote: The effect of neutralization capacity of Multivalent DARPin was evaluated by exposing serial dilutions of the DARPin candidates to increasing titers of SARS-CoV-2 and determining cell protection by CellTiter-Glo assay (Promega, Madison, USA). Serial dilution of DARPin candidates were prepared in 96 well plates in 100 µl cell culture medium (2%-FBS-MEM + HSA ...
-
bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 90% methanol/100 mM TEAB 10 times prior to on-column digestion with 1 ug Trypsin/Lys-C in 50 mM TEAB (47° C, 2 hours; Cat. #V5073, Promega, Madison, WI). The resulting peptides were eluted with sequential washes of 50 mM TEAB ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
-
bioRxiv - Biophysics 2020Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA with oligo (dT)15 primers and GoScript™ Reverse Transcriptase (Promega, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
-
bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
-
bioRxiv - Physiology 2023Quote: ... UMR106 cells were plated at 4 × 105 per well in a 6-well plate and transfected with 2 µg of NFAT5 gRNAs per well using the FuGENE® HD Transfection Reagent (Promega, USA). 24 h post-transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A) at 37°C for overnight ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR products were extracted from a 2% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified by QuantiFluor™-ST (Promega, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for an additional 2 – 6 days and cell growth was quantified using CellTiter-Glo® 2.0 Reagent (Promega, Cat. #924C). For immunoblot analysis ...
-
bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed 2 times with phosphate-buffered saline (PBS) and lysed with Luciferase Cell Culture Lysis 5x reagent (Promega, Madison, WI). Using the Nano-Glo Luciferase Assay System (Promega ...
-
bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
-
bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
-
bioRxiv - Plant Biology 2024Quote: ... The Halo-DREB2D fusion protein was obtained by coupled in vitro transcription/translation for 2 h at 27 °C using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, WI, USA). Halo-DREB2D synthesis was verified by Western blot using Anti-HaloTag® Monoclonal Antibodies (Promega) ...
-
bioRxiv - Developmental Biology 2024Quote: 3’ UTR fragments of miRNA target genes (for primer sequences see Table S2) were cloned into the XhoI/NotI sites downstream of the synthetic Renilla luciferase gene of psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... the samples were then diluted 4-fold with 100 mM ammonium bicarbonate to reduce urea concentration to 2 M and then added 5% (W/W) modified trypsin (Promega, Madison, WI). The pH was adjusted to 8.0 with 250 mM ammonium bicarbonate ...
-
bioRxiv - Cell Biology 2024Quote: ... clarified by centrifugation (500 x g, 5 min, at 15°C) and mixed 1:1 with 2× passive lysis buffer (Promega cat #E1941). Cell monolayers were washed in PBS and lysed in 1× passive lysis buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, M1705, Promega, Wisconsin, USA), random primers (10 ng/μl ...
-
bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was measured using the caspase-glo® 3/7 Assay system (Promega) 48 hours after drug treatment ...
-
bioRxiv - Cancer Biology 2021Quote: Caspase-3/7 activity was detected using a luminometric assay kit (Caspase-Glo 3/7; Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was measured using the Caspase-Glo® 3/7 Assay (Promega, # G8090) according to manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2020Quote: ... Caspase 3/7 activity was assessed by the Caspase-Glo® 3/7 Assay kit (Promega), following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: Caspase 3/7 activation assays were performed using the Caspase-Glo 3/7 Assay System (Promega), 72 hours after treatment with CT7001 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Caspase 3/7 activation was measured using the Apo-ONE Homogenous Caspase-3/7 Assay (Promega), following the manufacturers protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Caspase 3/7 activity was then assessed using Caspase-Glo 3/7 Assay System (Promega, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2023Quote: Cell viability was assessed by measuring caspase 3/7 activity (Caspase-Glo 3/7 Assay; Promega) in a 96-well format at 72 hours post-transfection as previously described13 ...
-
bioRxiv - Physiology 2023Quote: ... caspase 3/7 activity was measured using Apo-ONE® Homogenous Caspase 3/7 Assay (Promega). VSMC were grown on a 96 well plate and 50 µl of the reagent was added to 50 µl of medium in each well ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 activities were detected by Apo-ONE Homogeneous Caspase-3/7 Assay kit (Promega) according to the manufacturer’s protocols.
-
bioRxiv - Bioengineering 2023Quote: ... Caspase-3/7 activity was measured 96 h after treatment using Caspase 3/7-Glo (Promega) according to the manufacturer’s directions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 activity was measured using the Caspase-Glo 3/7 Assay Kit (Promega G8091). Pre-treatment of cells with EGF ...
-
bioRxiv - Cancer Biology 2024Quote: ... caspase 3/7 activity was measured using Caspase-Glo® 3/7 Assay System- (Promega, G8091) per manufacture’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Caspase 3/7 activity was then assessed using the Caspase-Glo 3/7 Assay (Promega, G8091) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... and pAdVAntage (3 µg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and pAdVAntage (3 µg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 3 mM of MgCl2 (Promega, USA), 10 pM of each primer ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Caspase 3/7 assay (Promega), respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caspase-Glo 3/7 assay (Promega) was used according to the manufacturer’s manual ...
-
bioRxiv - Biophysics 2023Quote: ... and 3 µM CA-JF646 (Promega). Cells were then imaged at room temperature with a 60x objective (N.A ...
-
bioRxiv - Developmental Biology 2022Quote: ... and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 3 ng/μl trypsin (Promega) were added to the dried pieces and incubated on ice for at least 40 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity (Promega, G8091), and Nucgreen Dead 488 staining (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... 3 µl of Trypsin gold (Promega, resuspended at 1 µg/µl in 50 mM Acetic acid ...
-
bioRxiv - Cell Biology 2024Quote: ... FuGene HD (Promega, E2311, 1:3).
-
bioRxiv - Developmental Biology 2024Quote: ... and pAdVAntage (3 μg, Promega, E1711) using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...