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Citations for IBA Life Sciences :
1 - 41 of 41 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... a biotin-blocking solution (IBA lifesciences) was added and incubated with the medium for 15 min at 4 °C under gentle stirring ...
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Cell Biology 2023Quote: ... pulldown buffer supplemented with 50mM biotin (IBA Lifesciences GmbH) formulated in elution buffer (1M Tris-Cl pH 8 ...
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bioRxiv - Immunology 2021Quote: ... or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences). To select for non-RBD binders ...
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bioRxiv - Biochemistry 2021Quote: ... Twin-Strep-tagged mTOR was captured onto a Strep-Tactin XT (IBA Lifesciences) coated surface prepared according to the supplied instructions ...
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bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... pH 8.0 and supplemented with a biotin blocking solution (IBA Lifesciences). RBDs were purified by affinity chromatography on a Strep-Trap HP 5 ml column followed by elution with 50 mM biotin and buffer exchange into PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Bound complex was eluted using Buffer BXT containing 50 mM biotin (IBA Lifesciences). Protein samples were diluted into SDS-PAGE lysis buffer and incubated at 99 °C for 10 min for separation by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... twin-strep tagged MKM containing lysates were incubated with Streptactin XT magnetic bead slurry (IBA Lifesciences) and purified according to manufacturer’s recommendation ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Immunology 2023Quote: ... TwinStrep tagged 8.9F was purified via gravity column before being eluted by 1X BXT (IBA Life Sciences) while 6x-His tagged 8.9F and 18.5C culture supernatants were rolled overnight with Ni-NTA beads at 4°C before using gravity columns to capture the resin ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA) and the kinase was eluted in 1x Biotin elution buffer BXT (IBA lifesciences). Plk1 was isolated from bacteria ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Biophysics 2023Quote: ... the tagged d1_d2 was purified from the supernatant by affinity chromatography using Strep-Tactin resin (IBA Lifesciences, Göttingen, Germany).
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin XT beads (2-5010-025) and biotin (0-1016-002) were purchased from IBA Lifesciences (Germany). Dihydroartemisinin (DHA ...
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bioRxiv - Microbiology 2024Quote: ... 1mM EDTA and 2 mM biotin) and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 12% TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Immunology 2020Quote: ... was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences). Plates were developed with TMB One Component (Surmodics ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The S6Pecto protein was expressed in FreeStyle293 cells and isolated on a StrepTrap HP column following the addition of BioLock Biotin Blocking Solution (IBA Lifesciences) to the culture supernatant.
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
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bioRxiv - Microbiology 2023Quote: Secreted twin StrepTagged gp16-V1V2 proteins in the harvested and filtered supernatant were supplemented with BioLock biotin blocking solution (IBA Lifesciences) at 5 µl/ml to mask the biotin present in the supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...