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Citations for IBA Life Sciences :
1 - 24 of 24 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA) and the kinase was eluted in 1x Biotin elution buffer BXT (IBA lifesciences). Plk1 was isolated from bacteria ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Histones H2A.S2 (bearing a C-terminal StrepII-tag, IBA Lifesciences) and H2B were expressed individually ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was centrifuged (30 min, 60,000xg, 4°C) and the supernatant was incubated with strep-tactin resin (Strep-Tactin® resin, Iba Lifesciences) for 30min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... which were incubated for 30 min at 4°C with 50 ul of ferrimagnetic StrepTactin beads (MagStrep Type3 XT beads, IBA LifeSciences) preincubated in 0.1M Tris pH8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 30,000 x g for 40 min at 4 °C in a JA 20 rotor and the supernatant was mixed with Strep-Tactin resin (IBA Lifesciences) previously equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 50,000 g for 30 minutes at 4°C and applied to pre-equilibrated StrepTactin resin (IBA Life Sciences). The soluble extract was incubated with the resin for one hour at 4°C with constant rotation ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Biophysics 2019Quote: ... APC/C was expressed with a Twin-Strep(II)-tag on the C-terminus of APC4 and purified using Strep-Tactin Sepharose (IBA Life Sciences) affinity chromatography followed by ion exchange chromatography and SEC ...
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bioRxiv - Biochemistry 2023Quote: ... Then the lysates were centrifuged at 15,000 x g for 20 min at 4°C and the supernatant was passed over a column packed with Strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...