Labshake search
Citations for IBA Life Sciences :
1 - 28 of 28 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... MEXi 293E cells (IBA Lifesciences) were cultured in MEXi culture medium (IBA Lifesciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... TWIN-Strep tagged proteins were then purified by incubating whole cell lysates from the transiently-transfected cell lines (0.5 ml) with 50 μl of MagStrep “type3” XT beads (IBA Life Sciences) for two-four hours at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein was expressed as described for RBD and NTD antigens in Expi293 cells and purified from mammalian cell culture supernatant using Strep-Tactin immobilized affinity resin (IBA Lifesciences). The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
-
bioRxiv - Biochemistry 2021Quote: ... The cell lysate passed over a Strep-Tactin column (IBA Lifesciences, Goettingen, Germany) pre- equilibrated with a binding buffer (10 mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Clarified cell lysates were incubated with prewashed Strep-Tactin beads (IBA Life Sciences) and allowed to bind for 2 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cell culture supernatants were applied to a Strep-tactin column (IBA Lifesciences GmbH, Göttingen, Germany) and eluted with 2.5 mM desthiobiotin ...
-
bioRxiv - Cell Biology 2022Quote: Transfection of 3T3 cells was carried out using the MatraTM magnetic bead transfection system (IBA Lifesciences) to warrant nuclear membrane integrity for subsequent FRAP experiments ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were either kept untreated or were incubated with CMV pp65 antigen peptide (IBA Lifesciences) for 1 h at 37°C and washed once with medium ...
-
bioRxiv - Developmental Biology 2024Quote: ... the cell lysate was loaded onto preequilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences GmbH, Germany) and washed with wash buffer (500 mM NaCl ...
-
bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted and clear lysate was loaded on Strep-Tactin® resin (IBA Lifesciences) pre-equilibrated with Strep Buffer (50 mM HEPES pH 8.0 ...
-
bioRxiv - Immunology 2021Quote: ... before staining cells with DY-549-labeled strep-tactin (1:100 dilution, IBA lifesciences, Cat #2-1565-050) or Allphycocyanin-labeled streptavidin (1:200 dilution ...
-
bioRxiv - Biochemistry 2023Quote: ... After equilibration of the Strep-tactin XT Superflow resin (IBA lifesciences, 10 mL per liter of cell supernatant) in 0.1 M Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates from cells transfected with Sb1 were directly loaded onto 100-300 µl of Streptactin superflow resin (IBA LifeSciences) to purify SBP-tagged sybodies ...
-
bioRxiv - Immunology 2024Quote: ... B cells were stained with rGP38 (IbAr10200) that had been tetramerized at 25 nM using Streptactin-PE (IBA Lifesciences) and Streptactin-APC (IBA Lifesciences) ...
-
bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
-
bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
-
bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Primary CD4+ T cells were harvested by positive selection using a FABian automated enrichment system and CD4 isolation kit (IBA Lifesciences). Isolated T cells were suspended in complete RPMI-1640 medium supplemented with 5 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... The S6Pecto protein was expressed in FreeStyle293 cells and isolated on a StrepTrap HP column following the addition of BioLock Biotin Blocking Solution (IBA Lifesciences) to the culture supernatant.
-
bioRxiv - Immunology 2022Quote: ... Both S trimer and S2 trimer were expressed in the FreeStyle 293F cells and purified by Strep-Tactin Sepharose (IBA Lifesciences) followed by gel filtration chromatography (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2022Quote: We transfected pStrep-ANKLE1 plasmid into HEK293T cells for 24 hours and performed affinity purification MagStrep3XTBeads and BXT elution buffer (IBA Lifesciences) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was then batch-incubated with 250 μL bed volume per L cell culture of Strep-Tactin agarose (IBA Lifesciences) pre-equilibrated with wash buffer (20 mM HEPES ...
-
bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
-
bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
-
bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...