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Citations for IBA Life Sciences :
1 - 47 of 47 citations for 6 Isobutoxy 5 methylpyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% suspension (IBA Lifesciences) and eluted with desthiobiotin ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
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bioRxiv - Molecular Biology 2023Quote: ... from 0 mM to 5 mM desthiobiotin (IBA Lifesciences GmbH), was then used to elute each complex ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was added to magnetic Strep beads (MagStrep type 3 beads, IBA Life science) previously equilibrated with buffer A and incubated for 30 min at 4 °C on a roller ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed magnetic Streptactin beads (100 μl, MagStrep type 3 XT, IBA Life Sciences, Gottingen, Germany) were added and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 5 mL Strep-Tactin Sepharose (IBA Lifesciences) at 4 C overnight ...
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bioRxiv - Genomics 2020Quote: ... 800 μL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... 800 µL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The bound protein was eluted in 3 CV buffer W supplemented with 2.5 mM desthiobiotin (IBA Life Sciences). This material was then applied to a MonoQ 5/50 GL (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with 5 mL of equilibrated StrepTactin Superflow resin (IBA Lifesciences). The resin was washed and equilibrated with Buffer A containing 0.02% DDM and 0.004% CHS ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was loaded on a 5 mL StrepTactin XP column (IBA life sciences), washed with 25 mL Strep-A buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Lysate was loaded on 5 ml of Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) and passed through the resin by gravity flow ...
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... FLAG beads were rinsed with the same buffer for an additional elution which was combined with the initial peptide elution and incubated with MagStrep “type 3” magnetic bead suspension (IBA Lifesciences) for 2h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... coli MG1655 genomic DNA with primers 5’ CTC GGT CTC CAA TGA ATA AAT ATC AGG CAG TGA 3’ and 5’ CTC GGT CTC AGC GCT TTA TTT GAC TAA TGA AAA TAG ATC A 3’ and cloned into the Eco31I (BsaI) sites of plasmid pPR-IBA101 (IBA Lifesciences) to yield plasmid pRCLA10 ...
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bioRxiv - Neuroscience 2021Quote: ... human SARM1 without mitochondria targeting sequence and with N-terminal tandem StrepTag II was expressed in HEK293T cells expressing NRK1 supplemented with NR (Essuman et al., 2017) and purified with MagStrep (Strep-Tactin) type 3 XT beads suspension (IBA Lifesciences). The purified protein attached on beads was incubated with NAD+ (10 μM for Figure 5 B ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG-solubilized lysates were added to 5 μg of TBS-prewashed MagStrep XT magnetic beads (IBA Lifesciences) for 1 h with mild shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The S protein was eluted by Buffer A containing 0.02% DDM and 5 mM desthiobiotin (IBA Lifesciences). The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 mL polypropylene column was packed with 0.5 mL Strep-Tactin XT 4Flow resin (IBA Life Sciences), overlaid with 0.5 mL Strep wash buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Culture supernatants were harvested 5 days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested 5 days post transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs were then added to pre-washed (PBST) and pre-blocked (5% BSA in PBST) magnetic streptavidin beads (Dynabeads M-280, Thermo) or Streptactin-agarose (IBA Lifesciences), with the amount of beads used being tenfold greater than what would be theoretically required based on the manufacturer’s listed binding capacity ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...