Labshake search
Citations for IBA Life Sciences :
1 - 48 of 48 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... 5% suspension (IBA Lifesciences) and eluted with desthiobiotin ...
-
bioRxiv - Biochemistry 2024Quote: ... the ZnF was expressed as a fusion construct with an N-terminal Twin-Strep-tag® (IBA Lifesciences, Göttingen) and purified as the other constructs.
-
bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
-
bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
-
bioRxiv - Molecular Biology 2023Quote: ... from 0 mM to 5 mM desthiobiotin (IBA Lifesciences GmbH), was then used to elute each complex ...
-
bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
-
bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 5 mL Strep-Tactin Sepharose (IBA Lifesciences) at 4 C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
-
bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with 5 mL of equilibrated StrepTactin Superflow resin (IBA Lifesciences). The resin was washed and equilibrated with Buffer A containing 0.02% DDM and 0.004% CHS ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluate was loaded on a 5 mL StrepTactin XP column (IBA life sciences), washed with 25 mL Strep-A buffer (150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: Lysate was loaded on 5 ml of Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) and passed through the resin by gravity flow ...
-
bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
-
bioRxiv - Biochemistry 2020Quote: ... LMNG-solubilized lysates were added to 5 μg of TBS-prewashed MagStrep XT magnetic beads (IBA Lifesciences) for 1 h with mild shaking ...
-
bioRxiv - Biochemistry 2022Quote: ... The S protein was eluted by Buffer A containing 0.02% DDM and 5 mM desthiobiotin (IBA Lifesciences). The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
-
bioRxiv - Molecular Biology 2024Quote: ... a 5 mL polypropylene column was packed with 0.5 mL Strep-Tactin XT 4Flow resin (IBA Life Sciences), overlaid with 0.5 mL Strep wash buffer (100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
-
bioRxiv - Biophysics 2019Quote: ... The lysate was centrifuged (30 min, 60,000xg, 4°C) and the supernatant was incubated with strep-tactin resin (Strep-Tactin® resin, Iba Lifesciences) for 30min at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... which were incubated for 30 min at 4°C with 50 ul of ferrimagnetic StrepTactin beads (MagStrep Type3 XT beads, IBA LifeSciences) preincubated in 0.1M Tris pH8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 30,000 x g for 40 min at 4 °C in a JA 20 rotor and the supernatant was mixed with Strep-Tactin resin (IBA Lifesciences) previously equilibrated with buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: nProtein A Sepharose 4 Fast Flow resin (Cytiva) was used for affinity purification of adalimumab whilst Strep-Tactin XT 4Flow resin (IBA Lifesciences) was used for Strep-II tagged-tagged proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
-
bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 50,000 g for 30 minutes at 4°C and applied to pre-equilibrated StrepTactin resin (IBA Life Sciences). The soluble extract was incubated with the resin for one hour at 4°C with constant rotation ...
-
bioRxiv - Microbiology 2022Quote: ... Culture supernatants were harvested 5 days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested 5 days post transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... Then the lysates were centrifuged at 15,000 x g for 20 min at 4°C and the supernatant was passed over a column packed with Strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with lysis buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
-
bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
-
bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
-
bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
-
bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
-
bioRxiv - Biochemistry 2023Quote: ... Constructs were then added to pre-washed (PBST) and pre-blocked (5% BSA in PBST) magnetic streptavidin beads (Dynabeads M-280, Thermo) or Streptactin-agarose (IBA Lifesciences), with the amount of beads used being tenfold greater than what would be theoretically required based on the manufacturer’s listed binding capacity ...
-
bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
-
bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
-
bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...