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Citations for IBA Life Sciences :
1 - 25 of 25 citations for 6 Bromo benzo d isoxazole 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The SBP-FKBP12.6-RyR2 complex was eluted with the wash buffer supplemented with 2.5 mM D-desthiobiotin (Iba Lifesciences). After checking the purity by SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was added to magnetic Strep beads (MagStrep type 3 beads, IBA Life science) previously equilibrated with buffer A and incubated for 30 min at 4 °C on a roller ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed magnetic Streptactin beads (100 μl, MagStrep type 3 XT, IBA Life Sciences, Gottingen, Germany) were added and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 800 μL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... 800 µL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The bound protein was eluted in 3 CV buffer W supplemented with 2.5 mM desthiobiotin (IBA Life Sciences). This material was then applied to a MonoQ 5/50 GL (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... FLAG beads were rinsed with the same buffer for an additional elution which was combined with the initial peptide elution and incubated with MagStrep “type 3” magnetic bead suspension (IBA Lifesciences) for 2h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... coli MG1655 genomic DNA with primers 5’ CTC GGT CTC CAA TGA ATA AAT ATC AGG CAG TGA 3’ and 5’ CTC GGT CTC AGC GCT TTA TTT GAC TAA TGA AAA TAG ATC A 3’ and cloned into the Eco31I (BsaI) sites of plasmid pPR-IBA101 (IBA Lifesciences) to yield plasmid pRCLA10 ...
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bioRxiv - Neuroscience 2021Quote: ... human SARM1 without mitochondria targeting sequence and with N-terminal tandem StrepTag II was expressed in HEK293T cells expressing NRK1 supplemented with NR (Essuman et al., 2017) and purified with MagStrep (Strep-Tactin) type 3 XT beads suspension (IBA Lifesciences). The purified protein attached on beads was incubated with NAD+ (10 μM for Figure 5 B ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...