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Citations for Amresco :
1 - 27 of 27 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco) and transfered to a PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and the percentage of FRH in the final product was visually estimated based on the percentage of the FRH band relative to other bands (∼30% FRH) when the eluted protein was visualized on a Coomassie-stained gel (Amresco, 0472-25G). Expression and purification of FRHΔ100 was verified by SDS-PAGE analysis (ThermoScientific ...
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bioRxiv - Microbiology 2020Quote: ... agarose gel (Amresco, Solon, OH, USA) made with 0.5% Tris-borate-EDTA buffer (Amresco ...
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bioRxiv - Microbiology 2020Quote: ... gel with 0.5% EDTA buffer (Amresco, Ohio). SYBR Gold (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... The polyacrylamide gel was stained with 0.02% ethidium bromide (Amresco) and imaged a Gel Doc XR+ System (Bio-Rad).
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bioRxiv - Microbiology 2019Quote: ... gel with 0.5% Tris-Borate-EDTA buffer (Amresco, Solon, OH, USA). SYBR Gold (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Gels were stained with 2 µg/mL ethidium bromide (Amresco, Solon, OH) in 1 X TBE and rocked for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... the gel was stained with Coomassie Brilliant Blue R-250 (AMRESCO, 0472-10G) and Western blotting using anti-His (Transgen Biotech ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments between 400-700 bp were selected on a 2% agarose gel (Amresco) and purified using a QIA quick Gel Extraction Kit (Fermentas ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were visualized via gel electrophoresis on a 1.5% w/v agarose (Amresco, Ohio) gel with 0.5% EDTA buffer (Amresco ...
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bioRxiv - Microbiology 2019Quote: PCR products were visualized via gel electrophoresis on a 1.5% w/v agarose (Amresco, Solon, OH, USA) gel with 0.5% Tris-Borate-EDTA buffer (Amresco ...
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bioRxiv - Cell Biology 2019Quote: ... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Biophysics 2024Quote: ... Protein estimation was done using the Bradford’s method using Bradford reagent (Amresco) [1-3] ...
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bioRxiv - Genetics 2022Quote: ... PCR products were size fractionated on a 1.5% agarose gel and stained with EZ-VisionR One (AMRESCO, Solon, OH). The variations in mRNA levels of the target genes were established by relative RT-qPCR with a CFX96TM Real-Time PCR Detection System and the iQ™ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Labelled DNA was added and the mixture was left at RT for another 20 min before loading on a 5% polyacrylamide gel (Acryl/ Bis 29:1, Amresco) eluted with 1x TAE (40 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... The slope and the offset of the linear function were used to determine the protein concentration of each sample and then the samples were diluted to 1 µg/µl with sample (M260 Next Gel Sample loading buffer 4x, Amresco) and Laemmli buffer (Cayman Chemical) ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with final concentration of 0.2% (w/v) L-arabinose (AMRESCO) at 37°C when OD600 reached 0.4~0.6 ...
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bioRxiv - Physiology 2021Quote: ... lysed in protein loading buffer (50 mM Tris, pH 6.8, 100 mM dithiothreitol (Amresco, 0281), 2% SDS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
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bioRxiv - Biochemistry 2019Quote: ... For unlabeled protein expression was induced with 1 mM isopropylthio-β-D-galactoside (IPTG; Amresco, 97061) and cultures were grown for 18 hours at 18°C ...
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bioRxiv - Immunology 2020Quote: ... Native cell wall proteins were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... approximately equal loading and transfer was confirmed by total protein staining of the nitrocellulose membrane with Ponceau S (Amresco). Chemiluminescent detection followed incubation with polyclonal antiserum or monoclonal antibody ...
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bioRxiv - Immunology 2020Quote: Supernatant proteins in the native form were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the beads were washed with lysis buffer and proteins were eluted in 2x Laemmli loading buffer containing 1 mM dithiothreitol (DTT; Amresco, Solon, OH) for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred from the gel to 0.45 μm pore size nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...