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Citations for Amresco :
1 - 28 of 28 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Nonspecific binding sites were blocked with PBS supplemented with 3% BSA (Amresco Inc., Solon, OH, USA) and 10% goat serum (Vector Labs ...
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bioRxiv - Cell Biology 2023Quote: ... were added to the chamber in the binding buffer: BRB80 supplemented with 1 mM TCEP (AMRESCO, Solon, OH), 0.02 mg/ml κ-casein (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 mM glucose (AMRESCO, 0188)) ...
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bioRxiv - Biochemistry 2024Quote: ... in 4 ml of H3PO4 (Amresco E582-500ML) and 46 ml of concentrated sulfuric acid (Sigma 258105-500ML ...
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bioRxiv - Cell Biology 2019Quote: ... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Biophysics 2024Quote: ... Protein estimation was done using the Bradford’s method using Bradford reagent (Amresco) [1-3] ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed with 4 % paraformaldehyde for 5 mins and stained with X-gal compound (Amresco 0428) dissolved at 1mg/ml for 3-6hrs at 37°C as previously described15 ...
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bioRxiv - Biochemistry 2020Quote: ... PBS containing 2 μg/mL Hoechst 33342 and 4 μg/mL pyronin Y (Amresco, cat# 0207) was added to the cells to stain DNA and RNA ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco) and transfered to a PVDF membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted from Day 0 and Day 4 cells in biological triplicate using Ribozol (Amresco), then DNaseI treated with TURBO DNase (Invitrogen).
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with final concentration of 0.2% (w/v) L-arabinose (AMRESCO) at 37°C when OD600 reached 0.4~0.6 ...
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bioRxiv - Physiology 2021Quote: ... lysed in protein loading buffer (50 mM Tris, pH 6.8, 100 mM dithiothreitol (Amresco, 0281), 2% SDS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
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bioRxiv - Biochemistry 2019Quote: ... For unlabeled protein expression was induced with 1 mM isopropylthio-β-D-galactoside (IPTG; Amresco, 97061) and cultures were grown for 18 hours at 18°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The oligos were then ethanol precipitated by addition of 4 μL sodium acetate (3M, pH5.2, AMRESCO, Cat.E521-100 ml) and 110 μL 100% ethanol and cooled to −80c for at least 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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bioRxiv - Immunology 2020Quote: ... Native cell wall proteins were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Neuroscience 2022Quote: ... animals received a subcutaneous injection of Carprofen (Rimadyl®, Zoetis, 4 mg/kg of body weight, in 0.9% NaCl [Amresco]). Anaesthesia was induced by intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2020Quote: ... approximately equal loading and transfer was confirmed by total protein staining of the nitrocellulose membrane with Ponceau S (Amresco). Chemiluminescent detection followed incubation with polyclonal antiserum or monoclonal antibody ...
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bioRxiv - Immunology 2020Quote: Supernatant proteins in the native form were precipitated from the supernatant using 80% w/v (NH4)2SO4 (Amresco, OH) at 4 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the beads were washed with lysis buffer and proteins were eluted in 2x Laemmli loading buffer containing 1 mM dithiothreitol (DTT; Amresco, Solon, OH) for 30 min ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and the percentage of FRH in the final product was visually estimated based on the percentage of the FRH band relative to other bands (∼30% FRH) when the eluted protein was visualized on a Coomassie-stained gel (Amresco, 0472-25G). Expression and purification of FRHΔ100 was verified by SDS-PAGE analysis (ThermoScientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred from the gel to 0.45 μm pore size nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing, Sanford, ME; 1215471) and total protein visualized using Ponceau S (Amresco, Radnor, PA; K793). The membrane was blocked with 2.5% (wt/vol ...
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bioRxiv - Immunology 2022Quote: ... Transfected cells were allowed to recover for two days and were then labelled at 4°C with anti-Thy1.1 antibody (clone HIS51, eBiosciences) for 30 minutes in PBS buffer containing 0.1% BSA (Amresco). Cells were then washed in buffer and incubated with anti-mouse IgG microbeads beads (Miltenyi Biotech ...
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bioRxiv - Molecular Biology 2019Quote: ... one was used for Western blot to probe with Anti-Methyl-K antibody and the other half was used to stain with Coomassie Blue stain (Coomassie Brilliant Blue R-250, Amresco).