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Citations for Hyglos :
1 - 18 of 18 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... following the manufacturer’s instructions (Hyglos GmbH, Bernried, Germany). LPS removal involved three successive incubations of the preparations with the slurry ...
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bioRxiv - Microbiology 2024Quote: Endotoxins were purified on an affinity chromatography column (EndoTrap®HD, Hyglos, Bernried am Stranberger Seen, Germany), in which a bacteriophage-derived protein in the column matrix binds endotoxins with high affinity and specificity ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin concentration at different stages of the purification process was assessed using the EndoZyme® II (Lot 22599; Hyglos GmbH (bioMérieux) Germany ...
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bioRxiv - Immunology 2023Quote: ... All preparations were endotoxin-low (< 0,05 EU/mg) as determined by the EndoZyme® assay (Hyglos GmbH, Germany).
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bioRxiv - Immunology 2022Quote: ... LPS-free ovalbumin was from Hyglos, Germany (Cat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
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bioRxiv - Immunology 2022Quote: ... mice were first sensitized by intraperitoneal (i.p.) injections with 100 μg Endograde OVA (Hyglos) mixed with 100 μl alum (Alhydrogel ...
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bioRxiv - Immunology 2022Quote: ... 5×104 BMDCs were pulsed with 1 mg/ml endotoxin-free chicken egg ovalbumin (OVA, Hyglos GmbH, Germany) in the presence of 50 μg/ml MC38 TEVs or MLC-V ...
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bioRxiv - Bioengineering 2022Quote: ... which was performed with an EndoTrap Blue column (Hyglos GmbH, Munich, Germany). Purified ...
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bioRxiv - Microbiology 2021Quote: ... that was amplified on strain 536 and purified according to molecular biology protocols including an endotoxin removal step (Endotrap Blue, Hyglos, Germany). The stock solution of 8.2 log10 PFU/mL has an endotoxin concentration of 2.15 EU/mL.
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bioRxiv - Immunology 2021Quote: ... and used for vaccination studies as indicated in figure legends: endotoxin-free ovalbumin (OVA) from Hyglos GmbH [10 μg / 100 μl dose] ...
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bioRxiv - Immunology 2021Quote: ... mixed with 20 μg OVA (Ovalbumin, Hyglos, Germany) [15,21] ...
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bioRxiv - Immunology 2021Quote: ... Endotoxin was removed from purified adapters using the Endotrap® HD Endotoxin Removal System (Hyglos GmbH) and adapters were stored at −80°C in endotoxin-free Dulbecco’s PBS (Millipore TMS-012-A) ...
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bioRxiv - Immunology 2020Quote: ... endotoxin-free OVA (Hyglos #300036), or control minimal peptides (OVA257-264 and OVA323-339) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
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bioRxiv - Immunology 2020Quote: ... 1×105 MutuDC were seeded in round bottom 96-well plates and incubated for 5h with endotoxin-free OVA (Hyglos #300036) or grade VII OVA (Sigma Aldrich #A7641-250MG) ...
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bioRxiv - Immunology 2020Quote: ... or endotoxin-free OVA (Hyglos #300036). Minimal peptide OVA257-264 was used as a control for the capacity of DCs to activate T cells ...
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA, Hyglos, 321001), blue carrier protein (BCP ...