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Citations for Tecan :
1 - 31 of 31 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... A custom primer panel was developed by Tecan Genomics ...
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bioRxiv - Physiology 2021Quote: ... The optical density of each well was set at 450 nm (Tecan-Infinite ...
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bioRxiv - Plant Biology 2021Quote: ... set-up in 96-well plates using the Freedom EVO100 robot (Tecan, Switzerland). A no-template PCR negative control was included on each plate ...
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bioRxiv - Molecular Biology 2021Quote: ... “Excitation” was set to “Filter” for measuring using a Spark® 20M multimode reader (Tecan). The following formulae were used for calculating the app ...
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bioRxiv - Synthetic Biology 2022Quote: ... optimized gain manually set at 75) with a TECAN SAFIRE II plate-reader (TECAN, Switzerland). Cleaved sfGFP from the supernatant of cells trypsinized at OD 8 (gave bright bands from initial optimizations on bacteria cells ...
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bioRxiv - Microbiology 2022Quote: ... 96- well sitting drop plates were set using a Tecan Freedom EVO100 workstation (Tecan Group, Männedorf, Switzerland) by mixing 0.4μl of protein with 0.4μl of precipitant using the 96-reagent sparse-matrix screens JCSG+ and Structure Screen 1&2 (Molecular Dimensions ...
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bioRxiv - Bioengineering 2023Quote: ... Luminescent signals generated from each specific probe set were measured and quantified on a plate luminometer (Tecan). Each sample was assessed in 5 technical replicates ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... incubated at RT for 20 min and the OD595nm (Infinite F200 pro, Tecan) reading used to derive the protein concentration from a calibration curve (R2 = 0.99 ...
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bioRxiv - Microbiology 2022Quote: ... All qPCR reactions were set-up in triplicate in 96-well plates using the Freedom EVO100 robot (Tecan, Switzerland), with a triplicate no-template negative control included on each plate ...
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bioRxiv - Biochemistry 2023Quote: ... with excitation at 440 nm and emission intensity detection at 510 nm (bandwidth set to 20 nm) (Tecan; Mannedorf, Switzerland). Three technical replicates were carried out on the same plate.
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bioRxiv - Molecular Biology 2024Quote: ... AlamarBlue was added at 1:10 ratio to the cells and quadruplicates of each condition was measured every hour on the infinite M200 fluorescence plate reader set to 37°C (Tecan Systems) with excitation 570 nm ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were incubated at room temperature for 15 min and polarization was determined by exciting at 440 nm and measuring emission intensity at 510 nm with bandwidths set to 20 nm using a plate reader equipped with a monochromator (Tecan, Männedorf, Switzerland). Displacement of GFP-Tβ4 from actin monomers results in a decrease in polarization ...
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bioRxiv - Biochemistry 2023Quote: ... A baseline reading every min for 10 min with the temperature set at 30°C was taken at 340 nm in a plate reader (Tecan Safire2, TECAN Systems Inc.). After the addition of 25 μl 3 mM α-ketoglutarate in 50 mM Tris-HCl pH 7.8 (or 50 mM Tris-HCl pH 7.8 for “no substrate” controls) ...
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bioRxiv - Biochemistry 2023Quote: ... A baseline reading every min for 10 min with the temperature set at 30°C was taken at 340 nm in a plate reader (Tecan Safire2, TECAN Systems Inc.). After the addition of 25 μl 3 mM α-ketoglutarate in 50 mM Tris-HCl pH 7.8 (or 50 mM Tris-HCl pH 7.8 for “no substrate” controls) ...
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bioRxiv - Genetics 2019Quote: ... PCR reactions containing Lister427 gDNA according to manufacturer’s specifications and primer pairs specific to each ORF were transferred into the associated well number for each plate by TECAN Freedom EVO 150 pipetting instrument ...
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bioRxiv - Biophysics 2022Quote: ... Dose-response curves at different time points after storage of the samples at RT were obtained by LRET acquisitions on a Spark 20M (Tecan) through calculation of the ratio of the integrated sensitized acceptor emission at 535/25 nm at 100-500 µs over 1200-1600 µs after donor excitation at 340/20 nm.
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated for 30min at RT and the OD was measured by spectrophotometry at 570 nm (Tecan, Switzerland). Percent biofilm formation was calculated in comparison to PA14 culture that was grown in the absence of compounds.
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence signals were recorded following incubation for 30 min at RT using the Infinite M200 plate reader (Tecan, Männedorf, Switzerland).
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bioRxiv - Biochemistry 2023Quote: ... Capture of the m6A-modified RNA by the m6A reader and the biotinylated RNA by Streptavidin was allowed to proceed for 3 hours at RT and in the dark before the HTRF signal was measured using a Tecan Spark plate reader (Tecan). The plate reader recorded with a delay of 100 μs the emission at 620 and 665 nm after the excitation of the HTRF donor with UV light at 320 nm ...
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bioRxiv - Synthetic Biology 2022Quote: ... the PCR reaction master mix was prepared by Tecan FluentControl liquid handler in a 96-well format ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell viability was measured with 0.5% crystal violet (10 minutes at RT, followed by three PBS washes, 100uL of 100% EtOH solubilization and absorbance reading at 590nm using a Tecan Infinite F200 Pro reader).
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bioRxiv - Cancer Biology 2023Quote: ... Fluorescence signals of PCR products were measured using fluorescence plate reader (TECAN SpectraFluor Plus ...
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bioRxiv - Genomics 2022Quote: We prepared libraries for RNA sequencing from each RNA sample used in the RT-qPCR analyses according to Universal RNA-Seq with NuQuant + UDI (Tecan Genomics, Inc., Redwood City, CA) manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were prepared as instructed for automated Freedom EVO® PCR workstation (Tecan) and run in QuantStudio 7 Flex Thermo cycler (Applied Biosystem)
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were in 96-well plates with a Freedom EVO 200 (Tecan) robotic platform ...
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bioRxiv - Genomics 2024Quote: ... Bead-bound libraries were amplified with the PCR reagents from the Ovation Ultralow Library V2 Kit (Tecan). Libraries were quantified and sequenced on a Novaseq 6000 with 2×150bp configuration.
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bioRxiv - Genetics 2019Quote: ... PCR products were confirmed on 2% agarose gels and quantified the concentration using a SYBRGreen staining followed by Tecan Freedom Evo and Infinite Microplate Reader ...
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bioRxiv - Physiology 2023Quote: ... strand selection and library amplification by PCR using the Universal Plus mRNA-seq with UDI preparation kit (Tecan Genomics 0520-A01).
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bioRxiv - Microbiology 2021Quote: ... was collected in 200 μl PCR tubes and fluorescence intensity and phage activity was assayed using an Infinite200 PRO (Tecan, Grödig, Austria) fluorescent plate reader ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrations of purified PCR products were determined by absorbance measurements at a wavelength of 260 nm using an infinite F200 pro device (TECAN, Grödig, AT).
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bioRxiv - Synthetic Biology 2023Quote: ... The primers were further diluted to 5 μM and aliquoted into new 96-well PCR plates using Multi-Channel Arm (MCA) inside FluentControl 1080 liquid handler (Tecan, Männedorf, Switzerland) for sample preparation of PCR reactions ...