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Citations for BioLegend :
301 - 350 of 352 citations for 6 6' Di O tert butyldimethylsilyl D lactal since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... KIR3DL2 (clone FAB2878A; R&D) and KIR2DL5 (clone UP-R1; BioLegend). To exclude dead cells ...
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bioRxiv - Immunology 2021Quote: Immunofluorescence combining IL-33 (R&D AF3626) and CD45 (Biolegend 30-F11) antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone ...
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bioRxiv - Immunology 2024Quote: ... or a control isotype-matched antibody IgG (clone Poly4053) (BioLegend, R&D systems) were added at the primary phase (day 0 and 7 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... SCF (50 ng/ml) (R&D) and IL-3 (50 ng/ml) (BioLegend). For neutrophilic differentiation ...
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bioRxiv - Cancer Biology 2024Quote: TotalSeq-D Heme Oncology Panel of oligo-conjugated reagents were purchased from Biolegend (clone information can be found in Supplemental Methods) ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Pacific Blue-Annexin V and 7AAD (7-aminoactinomycin D) (Biolegend) before running in an LSRII cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 20 μg/mL 7-AAD solution (7-amino-actinomycin D, Biolegend, 650 nm) for 15 min at room temperature in dark ...
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bioRxiv - Cell Biology 2021Quote: ... PAC-1 FITC (1:12, D Bioscience) and annexin V APC (1:60, Biolegend) for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1M cells were stained with TotalSeq-D Heme Oncology Cocktail (BioLegend; Cat. num. 399906) reconstituted in 59 μl of Cell Staining buffer (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptotic B16.F10 cells were stained with 7-Amino-actinomycin D (7-AAD, Biolegend) to determine viability ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded using 7-aminoactinomycin D (7-AAD, eBioscience) and zombie violet (BioLegend). FACS analysis was conducted using a MACSQuant analyzer (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: The PE-Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit (BioLegend, UK) was used to detect apoptosis according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) was used to identify Lin- stromal and epithelial subpopulations ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-CD49f/APC (R&D, Cat.# FAB13501A) and anti-EpCAM/APC-Cy7 (Biolegend, Cat.# 118217) were used to identify the lineage- (Lin- ...
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bioRxiv - Biochemistry 2022Quote: ... and 15 μg/mL of 7-amino-actinomycin D (7-AAD) (BioLegend, San Diego, CA, USA) for 2 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with 7-Amino Actinomycin D viability staining solution (7-AAD, BioLegend, San Diego, CA). The suspensions were incubated for 45 min on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31 ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with recombinant IL-2 (R&D Systemts) at 10ng/mL and 4µg/mL of anti-CD28 antibody (Biolegend), plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells obtained from the BALF and homogenized lung tissue of naïve and infected animals were stained with 7-aminoactinomycin D (7-AAD, Cat. 420404, BioLegend) or SytoxTM Blue Dead Cell Stain (Cat ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... All staining were performed in FACS buffer and dead cells were counterstained with 7-amino-actinomycin D (7-AAD, Biolegend). Before analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Cell Biology 2024Quote: ... was then carried out following the Chromium Next GEM Single Cell 3’ protocol for v3.1 reagents from 10X Genomics (CG000206 Rev D) and TotalSeq™-A Antibodies and Cell Hashing protocol from Biolegend (available online at https://www.biolegend.com/en-gb/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol) ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 594 conjugated anti-hCD19 (mouse IgG1, R & D Systems) and Alexa Fluor 647 conjugated anti-human CD340 (erbB2/HER-2, Biolegend) antibodies were used for CEACAM5 ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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bioRxiv - Microbiology 2023Quote: ... Immunoblotting was performed as described previously30. Antibodies against Mtb Rho (a gift from D. Schnappinger, Weill Cornell Medicine) and against Eco RpoB (BioLegend, W0023) were used.
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bioRxiv - Neuroscience 2021Quote: Nrxn1 neuron cKO-T brain slices (coronal and sagittal) were labeled for Nrxn1 (Fig. 1c-d) using purified HA-647 (mouse, 1:500, Biolegend, Cat# 682404) as described above ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Receptor surface expression levels were verified by flow cytometry using CXCR4- and CXCR7-specific mAbs (12G5, R&D Systems and 8F11-M16, BioLegend) on a BD FACS Fortessa cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... Synomag-D+ DC were intradermally injected into each hind footpad immediately following Tag-It Violet™ (Violet+) cell tracking dye incorporation (Biolegend, San Diego, USA). MPI was done immediately following injection (day 0 ...