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Citations for BioLegend :
301 - 350 of 7710 citations for 1 4 Bromo 3 methylphenyl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells from the bone marrow were first stained with the following combination of biotinylated antibodies (lineage cocktail) for 30 minutes at 4 C: Gr-1 (RB6-8C5; Biolegend), Ter-119 (TER-119 ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 4°C with KI67 primary antibody (cell signaling technology) diluted 1:400 or CD8a primary antibody (Biolegend) diluted 1:200 in Antibody diluent signal stain (Dako) ...
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bioRxiv - Immunology 2023Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed in TBS-T and incubated overnight at 4 ◦C with monoclonal pNF-κB p65 (Ser 529)-specific antibody (1:500 dilution, clone 817403, Biolegend), monoclonal NF-κB p65-specific antibody (1:2500 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixation and permeabilization was then performed for 1 hour at 4°C using the CytoFast Fix/Perm Set (426803, Biolegend). After fixation/permeabilization ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C in a humidified chamber: mouse anti-acetylated tubulin (1:400, BioLegend, 802001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Immunology 2022Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...
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bioRxiv - Immunology 2022Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in 1x TBST and incubated overnight at + 4 °C: mouse anti-β-amyloid 1:1000 (6E10, #SIG-39320, BioLegend), rabbit anti-sAPPβ 1:100 (#18957 ...
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated with following primary antibodies at 4 °C overnight: rabbit anti-β-amyloid 1:1000 (6E10, #SIG-39320, BioLegend) to detect diffuse β-amyloid ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an 18-hour incubation at 4°C with mouse anti-6E10 antibody (1:1000 in 2% NHS, BioLegend #803001). After three PBS washes ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and incubated for 30 min at 4 °C with the following antibodies resuspended in FACS buffer (DPBS with 1% FBS): anti-CD11b-FITC (BioLegend, #101205), anti-CD49f-PE (BD Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-TNFRSF12A (clone ITEM-4, Biolegend) was utilized for (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 µg of anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] from Abcam (ab817) and 4 µg from BioLegend (664912) were used for each sample ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 ng/ml IL-4 (Biolegend) for 9 days ...
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bioRxiv - Microbiology 2020Quote: ... anti-CD4 (2µg/ml, RM4-4; Biolegend), anti-CD3 (2µg/ml ...
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bioRxiv - Immunology 2022Quote: ... anti-CTLA-4 (APC, clone BNI3 – BioLegend), anti-CD138 (APC-Cy-7 ...
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bioRxiv - Immunology 2022Quote: ... Cd11a (M17/4,BioLegend, San Diego, CA), Cd127 (SB/199,Invitrogen-ThermoFisher Scientific ...