2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine citations for BioLegend

Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...

Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...

Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neurons

Vaibhavi Kadam, et al., bioRxiv - Neuroscience 2024

Quote: ... SCF (50 ng/ml) (R&D) and IL-3 (50 ng/ml) (BioLegend). For neutrophilic differentiation ...

Surface-associated antigen induces B-cell permeabilization and lysosome exocytosis facilitating antigen uptake and presentation to T-cells

Fernando Y. Maeda, et al., bioRxiv - Cell Biology 2021

Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).

State-dependent protein-protein interactions mediating 4-1BB CAR Signaling

Samuel A. Ritmeester-Loy, et al., bioRxiv - Immunology 2022

Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...

Inosine Induces Stemness Features in CAR T cells and Enhances Potency

Dorota D. Klysz, et al., bioRxiv - Immunology 2023

Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.

Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1

Takeya Masubuchi, et al., bioRxiv - Immunology 2024

Quote: ... Secreted IL-2 in the supernatant was measured using Human or Mouse IL-2 ELISA Kit (BioLegend). All incubations were performed at 37 °C / 5% CO2.

Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1

Takeya Masubuchi, et al., bioRxiv - Immunology 2024

Quote: ... the amount of IL-2 in the medium was quantified using Human IL-2 ELISA kit (Biolegend) or CD69 expression on Jurkat cells was measured by FACS ...

Targets of influenza Human T cell response are mostly conserved in H5N1

John Sidney, et al., bioRxiv - Immunology 2024

Quote: ... while CD137 APC (2:100) and OX40 PE-Cy7 (2:100; Biolegend; clone Ber-ACT35; Cat# 350012) were used for CD4+ T cells.

The ESCRT protein CHMP5 restrains skeletal progenitor cell senescence by preserving endo-lysosomal-mitochondrial network

Xianpeng Ge, et al., bioRxiv - Cell Biology 2020

Quote: ... and anti-beta-actin monoclonal antibody (Biolegend) were used.

Notch Inhibition Enhances Morphological Reprogramming of microRNA-Induced Human Neurons

Kyle F. Burbach, Andrew S. Yoo, bioRxiv - Developmental Biology 2024

Quote: ... rabbit anti-BETA TUBULIN III (Biolegend 802001), and rabbit anti-MAP2 (Cell Signaling #4542) ...

Robust and Functional Immunity up to 9 months after SARS-CoV-2 infection: a Southeast Asian longitudinal cohort

Vo Hoa Thi My, et al., bioRxiv - Immunology 2021

Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...

Multimodal Mapping of Human Lymphopoiesis Reveals B and T/NK/ILC Lineages are Subjected to Cell-Intrinsic versus Flt3L-Dependent Regulation

Kutaiba Alhaj Hussen, et al., bioRxiv - Immunology 2022

Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...

Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

Seydou Keita, et al., bioRxiv - Immunology 2022

Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...

Type 1 interferon remodels normal and neoplastic hematopoiesis in human

Chhiring Lama, et al., bioRxiv - Cancer Biology 2022

Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...

Conserved epigenetic programming and enhanced heme metabolism drive memory B cell reactivation

Madeline J. Price, et al., bioRxiv - Immunology 2021

Quote: ... anti-IgD-APC-Cy7 (Biolegend, IA6-2), anti-CD38-PE-Cy7 (Biolegend ...

Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

Quote: ... anti-CD32-PE-Cy7 (FUN-2, Biolegend), anti-HLA-DR-BV711 (L243 ...

Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

Quote: ... anti-CD32-PE-Cy7 (FUN-2, Biolegend) and ...

Genetic models of latent tuberculosis in mice reveal differential control by adaptive immunity

Hongwei Su, et al., bioRxiv - Microbiology 2021

Quote: ... APC anti-IL-2 (JES6-5H4, BioLegend), BV785 anti-CD3 (17A2 ...