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Citations for BioLegend :
1601 - 1650 of 8187 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... supernatant was aspirated and the cells were incubated in blocking buffer mixture for 5min at 4°C (1uL anti-mouse CD16/32 TruStain Fcx (RRID: AB-1877271 AB-1574973, Biolegend Cat #: 101320) and 99uL FACS buffer (1% BSA + 0.05% sodium azide in HBSS w/o Ca2+/Mg2+)) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained in PermWash buffer (eBioscience) for 30 min at 4°C with Abs (purchased from BioLegend unless noted) against Bcl2 (BCL/10C4) ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Staining for surface markers of T cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng/ml Escherichia coli lipopolysaccharides (LPS) for 24 hrs to induce M1 polarization or 25 ng/ml IL-4 (BioLegend catalog #766202) and 25 ng/ml IL-13 (BioLegend catalog #571102 ...
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bioRxiv - Immunology 2024Quote: Staining for surface markers of immune cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with 1x Perm buffer (Tonbo) and stained for intracellular antigens for 30 mins at 4°C with the following antibodies: GranzymeB: AF647 (Biolegend, Clone: GB11), IL-2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Immunology 2023Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend, clone 17A2), CD44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... for 20 minutes at 4°C and stained in perm wash buffer with anti-IFNg PE or BV785 (ThermoFisher or BioLegend, clone: XMG1.2) for one hour at room temperature.
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibody cocktail for surface markers at 4 °C for 30 min and then fixed using True nuclear transcription factor buffer set (Biolegend, Cat # 424401) for 20 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... in RPMI complete medium in a 6-well plate that was pre-coated overnight at 4°C with anti-CD3 antibody (BioLegend Cat# 100340) at 100 pg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... 96-well high-binding plates were coated overnight at 4°C with 21h10 protein (500 ng per well) diluted in 1X coating buffer (from ELISA set, Biolegend Cat#430804), and blocked with PBS + 10% IFS for 24 hours at 4°C ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... CX3CR1 (Biolegend, 2A9-1), CCR7 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... CD4 (1:50; Biolegend). After washing ...
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bioRxiv - Cell Biology 2020Quote: ... PMP22 (BioLegend, 1:5,000), Cleaved caspase3 and LC3AB I/II (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500 (BioLegend, 901502); anti-CYTC ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1-PerCP (BioLegend), VCAM-PeCy7 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... IgG2b (RMG2b-1; Biolegend), IgG2a (R19-15 ...
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bioRxiv - Immunology 2021Quote: ... IgA (RMA-1; Biolegend). Wells were blocked by incubating DMEM media (Gibco ...
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bioRxiv - Immunology 2021Quote: ... IgG3 (RMG3-1; Biolegend), IgA (RMA-1 ...
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bioRxiv - Immunology 2021Quote: ... IgG2b (RMG2b-1; Biolegend), IgG2a (R19-15 ...
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bioRxiv - Immunology 2021Quote: ... FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19 ...
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bioRxiv - Immunology 2022Quote: ... Sca-1 (D7, BioLegend) or the appropriate isotype controls plus FcBlocker for 30 min ...
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bioRxiv - Immunology 2021Quote: ... FasL (Biolegend, NOK-1), GzmB (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... CD86 (GL-1, BioLegend), major histocompatibility complex II (MHC II ...
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bioRxiv - Immunology 2020Quote: ... and PD-1 (BioLegend). Tumor cells were stained with Cy5.5-CLTX ...
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bioRxiv - Immunology 2021Quote: ... CX3CR1 (Biolegend, 2A9-1), CCR7 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... CD68 (1:1000 Biolegend) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Aβ (1:250, Biolegend) and Iba1 (1:20 ...
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bioRxiv - Cancer Biology 2021Quote: ... CK14 (1:200; Biolegend), CK8 (1:10 ...
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bioRxiv - Microbiology 2020Quote: ... EpCAM (1:200, BioLegend), SiglecF (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... SiglecF (1:200, BioLegend) and CD24 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ(1-16)(BioLegend, San Diego ...
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bioRxiv - Physiology 2021Quote: ... ICAM1 (1:200, Biolegend); CD68 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... Gr-1 (BioLegend, 108417), B220 (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... 1:1000 (BioLegend 611802); anti-beta-tubulin ...
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bioRxiv - Cancer Biology 2021Quote: ... Sca-1 BV785 (BioLegend, clone D7 ...
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bioRxiv - Cancer Biology 2022Quote: ... PD-1 (BioLegend, 135217), and CXCR6 (BioLegend ...