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51 - 100 of 8186 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020

• Role of T cells in intrauterine administration of activated peripheral blood mononuclear cells in recurrent implantation failure

  Guillaume Ricaud, et al., bioRxiv - Immunology 2021

  Quote: ... anti-IL-4/BV510 (Biolegend, USA), anti-IL-17/BV711 (Biolegend ...
• Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks

  Jacob W. Freimer, et al., bioRxiv - Genomics 2021

  Quote: ... or CTLA-4 (Biolegend, Cat #349908). Cells sorted for IL2RA underwent surface staining according to the manufacturer’s protocol ...
• Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks

  Jacob W. Freimer, et al., bioRxiv - Genomics 2021

  Quote: ... or CTLA-4 (Biolegend, Cat #349908) and analyzed with an Attune NxT Flow Cytometer with a 96-well plate-reader.
• Robust SARS-CoV-2-specific T-cell immunity is maintained at 6 months following primary infection

  J Zuo, et al., bioRxiv - Immunology 2020

  Quote: ... and anti-IL-4-PE (Biolegend). All antibodies were purchased from Biolegend ...
• Expression of Tim-3 drives naïve Treg to an effector-like state with enhanced suppressive activity

  Hridesh Banerjee, et al., bioRxiv - Immunology 2020

  Quote: ... CTLA-4 clone UC10-4B9 (BioLegend), CD103 clone M290 (BD) ...
• Acute lymph node slices are a functional model system to study immunity ex vivo

  Maura C. Belanger, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4 (ELISA MAX standard, Biolegend) and TNFα (R&D Systems ...
• CTLA-4 tail fusion enhances CAR-T anti-tumor immunity

  Xiaoyu Zhou, et al., bioRxiv - Immunology 2023

  Quote: ... or anti-CTLA-4 antibody (Biolegend) at 37°C for 1 hour ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-human IL-4 (OA19A66, Biolegend) and anti-human IL-5 (TRFK5 ...
• Human cerebrospinal fluid single exosomes in Parkinson’s and Alzheimer’s diseases

  Koya Yakabi, et al., bioRxiv - Neuroscience 2023

  Quote: ... Aquaporin-4 (E5206) (BioLegend catalog #600352), CD11b (M1/70 ...
• A single-dose MCMV-based vaccine elicits long-lasting immune protection in mice against distinct SARS-CoV-2 variants

  Kristin Metzdorf, et al., bioRxiv - Immunology 2024

  Quote: ... CD11a (M17/4, BioLegend, CA, USA), CD62L (MEL-14 ...
• Soquelitinib, A Selective Inhibitor of Interleukin-2-Inducible T Cell Kinase (ITK), is Active in Several Murine Models of T Cell-Mediated Inflammatory Disease

  Lih-Yun Hsu, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IL-4 (11B11, Biolegend) and anti-mouse IFNγ (XMG1.2 ...
• Membrane positioning across antigen-induced synaptic contacts tunes CAR-T cell signaling and effector responses

  Fenglei Li, Kaushik Choudhuri, bioRxiv - Immunology 2023

  Quote: ... with 100ng/ml hIL-4 (Biolegend) 2days post transduction ...
• Lyophilized mRNA-lipid nanoparticle vaccines with long-term stability and high antigenicity against SARS-CoV-2

  Liangxia Ai, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 PE (Biolegend, cat.504104), and IL-5 APC (Biolegend ...
• Melanoma-intrinsic NR2F6 activity regulates anti-tumor immunity

  Hyungsoo Kim, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and V5-tag (7/4, Biolegend).
• PPAR-γ regulates the effector function of human T_H9 cells by promoting glycolysis

  Nicole L. Bertschi, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 (50 ng/ml) (BioLegend) for TH2 cells ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... IL-4 BV605 (BioLegend, clone: 11B11), and IL-17a BV711 (BioLegend ...
• Initial TCR Signal Strength Imprints GATA3 and Tbet Expression Driving T-helper Cell Fate Decisions

  Mohammad Ameen Al-Aghbar, et al., bioRxiv - Immunology 2024

  Quote: ... PE/Cy7-IL-4 (11B11, Biolegend), PerCP-Cy5.5-IFNγ (XMGL2 ...
• Limiting mitochondrial-derived ATP transfer to the cytosol enhances T-cell activation

  Omri Yosef, et al., bioRxiv - Immunology 2024

  Quote: ... and IL-4 (Biolegend, BLG-431104) cytokines using enzyme-linked immunosorbent assay (ELISA).
• Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia

  Lawrence J. Dooling, et al., bioRxiv - Bioengineering 2024

  Quote: ... or interleukin-4 (IL4, BioLegend 574302) to polarize to M1 and M2 states ...
• Enhancing and inhibitory motifs have coevolved to regulate CD4 activity

  Mark S. Lee, et al., bioRxiv - Immunology 2021

  Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• Proteomics reveals unique identities of human TGF-β-induced and thymus-derived CD4⁺ regulatory T cells

  Mark Mensink, et al., bioRxiv - Immunology 2022

  Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
• The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions

  Mark S. Lee, et al., bioRxiv - Immunology 2023

  Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• A genome-wide genetic screen uncovers novel determinants of human pigmentation

  Vivek K. Bajpai, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse TYRP1 (MEL-5) (1:500; 4°C overnight; BioLegend, 917801), rabbit MITF (1:200 ...
• Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T cell differentiation

  Mingeum Jeong, et al., bioRxiv - Immunology 2022

  Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (all from Biolegend), 20 ng/ml IL-6 ...
• Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T cell differentiation

  Mingeum Jeong, et al., bioRxiv - Immunology 2022

  Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (both from Biolegend), 30 U/ml rhIL-2 (NIH) ...
• T:B cell cooperation in ectopic lymphoid follicles propagates CNS autoimmunity

  Anna Kolz, et al., bioRxiv - Immunology 2023

  Quote: ... 4 % goat serum and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT in a humidified dark chamber ...
• βIII-Tubulin is a Brake on Extrinsic Cell-Death in Pancreatic Cancer

  John Kokkinos, et al., bioRxiv - Cancer Biology 2022

  Quote: ... βIII-tubulin (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and α-smooth muscle actin (αSMA ...
• Distinct sets of molecular characteristics define tumor-rejecting neoantigens

  Anngela C. Adams, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-APC (1:80; clone RM4-4; BioLegend Cat. No. 116013), CD4-BV421 (1:160 ...
• Distinct sets of molecular characteristics define tumor-rejecting neoantigens

  Anngela C. Adams, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-BV421 (1:160; clone RM4-4; BioLegend Cat. No. 116023), FoxP3-APC (1:20 ...
• Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence

  David J. Bean, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-human CD137 (4-1BB (1:100 dilution, BioLegend, 309803). After staining ...
• Two distinct durable human class-switched memory B cell populations are induced by vaccination and infection

  Cory A. Perugino, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human CD19 (BioLegend, Clone SJ25C1, APC-Cy7, 1:25, 4℃); anti-human CD27 (BD Biosciences ...
• An IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma

  Madelon M.E. de Jong, et al., bioRxiv - Immunology 2023

  Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
• Enhanced STAT5a activation rewires exhausted CD8 T cells during chronic stimulation to acquire a hybrid durable effector like state

  Jean-Christophe Beltra, et al., bioRxiv - Immunology 2022

  Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
• Macrophage-Based iNos Reporter Reveals Polarization and Reprogramming in the Context of Breast Cancer

  Javier A. Mas-Rosario, et al., bioRxiv - Immunology 2022

  Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
• Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures

  Ameen A. Salahudeen, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-TNFRSF12A (clone ITEM-4, Biolegend) was utilized for (Fig ...
• Synergistic activity of Nanog, Pou5f3, and Sox19b establishes chromatin accessibility and developmental competence in a context-dependent manner

  Liyun Miao, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 4 µg of anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] from Abcam (ab817) and 4 µg from BioLegend (664912) were used for each sample ...
• Antigen-derived peptides directly engage the unfolded-protein sensor IRE1α to curb cross-presentation by dendritic cells

  Ofer Guttman, et al., bioRxiv - Cell Biology 2021

  Quote: ... and 10 ng/ml IL-4 (Biolegend) for 9 days ...
• Suppression of Cytotoxic T Cell Functions and Decreased Levels of Tissue Resident Memory T cell During H5N1 infection

  Matheswaran Kandasamy, et al., bioRxiv - Microbiology 2020

  Quote: ... anti-CD4 (2µg/ml, RM4-4; Biolegend), anti-CD3 (2µg/ml ...
• High Frequencies of PD-1⁺TIM3⁺TIGIT⁺CTLA4⁺ Functionally Exhausted SARS-CoV-2-Specific CD4⁺ and CD8⁺ T Cells Associated with Severe Disease in Critically ill COVID-19 Patients

  Pierre-Gregoire Coulon, et al., bioRxiv - Immunology 2022

  Quote: ... anti-CTLA-4 (APC, clone BNI3 – BioLegend), anti-CD138 (APC-Cy-7 ...
• Heterotypic Influenza Infections Mitigate Susceptibility to Secondary Bacterial Infection

  Ellyse M. Cipolla, et al., bioRxiv - Immunology 2022

  Quote: ... Cd11a (M17/4,BioLegend, San Diego, CA), Cd127 (SB/199,Invitrogen-ThermoFisher Scientific ...
• Vaccine Elicitation of HIV Broadly Neutralizing Antibodies from Engineered B cells

  Deli Huang, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4 at 50ng/ml(Biolegend, #574306), anti-CD40 at 5 μg/ml (Thermo ...
• Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primates

  Lochana M. Seenappa, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 (PE, clone: 8D4-8, BioLegend), TNFα (BV605 ...
• Differential Nr4a1 and Nr4a3 expression discriminates tonic from activated TCR signalling events in vivo

  Emma Jennings, et al., bioRxiv - Immunology 2020

  Quote: ... TCRVβ5.1/5.2 APC (Clone MR9-4, BioLegend), B220 PerCPcy5.5 (Clone RA3-6B2 ...
• Acute lymph node slices are a functional model system to study immunity ex vivo

  Maura C. Belanger, et al., bioRxiv - Immunology 2020

  Quote: ... ELISA MAX detection anti-IL-4 (Biolegend), or detection TNFα (R&D Systems ...
• Antigen and Checkpoint Receptor Recalibration of T Cell Receptor Signal Strength

  Thomas A.E. Elliot, et al., bioRxiv - Immunology 2021

  Quote: ... CTLA-4 PE (clone UC10-4B9, BioLegend), OX40 APC (clone OX-86 ...
• The PD-1 checkpoint receptor maintains tolerance of self-reactive CD8 T cell in skin

  Martina Damo, et al., bioRxiv - Immunology 2021

  Quote: ... CD4 (clone GK1.5 or RM4-4, BioLegend), CD44 (clone IM7 ...
• Cellular iron governs the host response to malaria

  Sarah K. Wideman, et al., bioRxiv - Immunology 2023

  Quote: ... 10 ng/mL IL-4 (574302, BioLegend), 10 ng/mL IL-5 (581502 ...
• Dendritic cell-mediated responses to secreted Cryptosporidium effectors are required for parasite-specific CD8⁺ T cell responses

  Breanne E. Haskins, et al., bioRxiv - Immunology 2023

  Quote: ... CD11a PE (clone M17/4) from Biolegend; CD90.1 APC-ef780 (clone HIS51) ...
• A PD-1-ST2 axis controls Th2 effector function in tissue via a metabolic checkpoint

  Graham A. Heieis, et al., bioRxiv - Immunology 2023

  Quote: ... IL-4 (11B11) conjugated to BV421 (BioLegend); IL-5 (TRFK5 ...