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Citations for BioLegend :
601 - 650 of 2160 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... in diluted blocking buffer (1/5 in PBS) or its isotype control (Biolegend, Cat # 400132) at 4°C overnight ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Cell Biology 2020Quote: ... treated cells were harvested and stained with 5 μg/ml propidium iodide and Annexin V (Biolegend) in Annexin V-binding buffer (10 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Enriched CD4SP thymocytes were then stained with CD4-Pacific blue (RM4-5) fluorochrome-conjugated antibodies (BioLegend). FACS Aria IIu and FACS Aria II SORP (BD Biosciences ...
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bioRxiv - Genetics 2020Quote: Antibodies to the following antigens were used for flow cytometry: CD4 BV650 (RM4-5, #100545, Biolegend), CD8a PE-Cy7 (53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Cell Biology 2022Quote: ... BV711 Mouse anti mouse CD64 (Monoclonal; clone X54-5/7.1; Biolegend; 1:100; Cat#139311, FACS). BV421 Rat anti mouse CD68 (Monoclonal ...
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bioRxiv - Genomics 2022Quote: ... and extracellular stains with anti-mouse CD4-APC (1:400, clone RM4-5, BioLegend Cat#100516), anti-mouse TCRb chain-FITC (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... the cell suspension was incubated in 5 μgml−1 anti-CD16/32 (#101302, Biolegend, 1:200), stained with Live Dead Near IR (#L10119,ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... cells were first stimulated for 5 hours with either anti-CD3 antibodies (1 ug/mL, Biolegend) or MTB300 peptide megapool (21 ...
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bioRxiv - Immunology 2023Quote: ... 5 × 105 NPCs were labeled with viability dye marker (Zombie Aqua, Biolegend, San Diego, CA, USA) and blocked with PBS supplemented with 1% Fc Block (MACS ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... for 6 hours at 37°C with 5% CO2 in the presence of brefeldin A (Biolegend) and monensin (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... PBMC were prelabeled with 5 mM Tag-it Violet™ Proliferation and Cell Tracking Dye (Biolegend) in PBS with 0.1% FBS for 10 min in a 37°C water bath ...
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bioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
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bioRxiv - Immunology 2021Quote: ... 1-5 * 106 cells were stained with antibodies specific for surface markers Thy1.2 (clone 30-H12, BioLegend), Thy1.1 (clone OX-7 ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies diluted in blocking solution: 5% Normal Goat Serum (Biolegend, 927502), or 0.2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... An aliquot of cells (5 ×105) was labeled with a viability dye (BioLegend Zombie Aqua; Cat# 423101) and fluorochrome- conjugated antibodies for enumerating of activated T cells that expressed high levels of CD44 ...
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bioRxiv - Immunology 2021Quote: ... The cell were stained with 5 µl of PerCP/cy5.5-Armenian Hamster-IgG (Biolegend, USA, cat#400931), PE-Rat-IgG2a (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Immunology 2021Quote: Surface staining was performed using the following fluorescence-labeled antibodies against: CD4 (RM4-5; Biolegend, BD Biosciences), CD5 (53-7.3 ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 420 x g for 5 minutes and resuspended in anti-CD16/CD32 (Fc Block; Biolegend) diluted 1:50 in FACS buffer (PBS with 2% BSA and 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with antibodies diluted in a blocking solution of 5% Normal Goat Serum (BioLegend) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted at 220-300 x g and resuspended in 5 mL RBC lysis buffer (BioLegend) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... C3b binding was detected with PE-conjugated mouse monoclonal anti-C3b/iC3b (5 μg/mL, BioLegend #846103) and C5b-9 membrane deposition/insertion was detected with mouse monoclonal anti-C5b-9 antibody (8 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... cytokines (hIL-10 20ug, mIL-15 15ug bid, IFN-γ 5×104 IU from BioLegend Inc., CA) were injected intraperitoneally in mice on day 1 ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining lysate (800 μl) was incubated with 5 μl of mouse anti-HA antibody (Biolegend #901514) with rocking for 4 hours at 4°C followed by addition of 200 μl of magnetic beads (Pierce #88803 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a secondary incubation with 5 µg/mL anti-HA-Alexa Fluor 647 (clone 16B12, BioLegend) and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... All samples were then blocked with 5 μg/mL anti CD16/32 (93; BioLegend Cat# 101301, RRID:AB_312800) and heat-inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were treated for 5 min with TruStain FcXTM (anti-mouse CD16/CD32) (cat. #101320, BioLegend) prior to staining ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Microbiology 2023Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...