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Citations for Roche :
1 - 36 of 36 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Biophysics 2022Quote: ... synthesized by IDT) with 66 mM digoxigenin N-hydroxysuccinimide ester (Roche) in 50 mM HEPES-NaOH ...
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bioRxiv - Genetics 2021Quote: ... 0.8× LightCycler 480 ResoLight Dye (Roche Applied Science GmbH ...
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bioRxiv - Cell Biology 2024Quote: SYBR Green I dye (Roche, Mannheim, Germany)
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bioRxiv - Cell Biology 2020Quote: ... DNA-binding (double strand) SYBR Green dye (Roche FastStart Essential DNA Green Master 11560300).
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bioRxiv - Microbiology 2021Quote: Antibodies and dye used: anti-HA (Roche clone 3F10), VVL-FITC (Vector FL1231) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) with 2× SYBR green dye (Roche) on a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... SYBR Fast MasterMix (2x) Universal Dye (#KK4602) from Kapa Biosystems was used in an Eppendorf Realplex2 Mastercycler ...
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bioRxiv - Cancer Biology 2021Quote: We established single-cell suspensions of surgically resected tumors by taking a small piece of PDAC tumor tissue collected in a cell-dissociation media [5 ml of minimal essential media (MEM) containing 100μl of Liberase-TM (2.5 mg/ml stock solution Roche/Sigma Aldrich Cat# 5401046001), 50μl of Kolliphor®P 188 (15 mM stock solution Sigma Cat# K4894) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... using the FastStart Essential DNA Green Master dye and polymerase (Roche). The final volume for each reaction was 10 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 M Urea loading dye was supplemented with complete protease inhibitor (Roche). 100 μl Urea loading dye were used to resuspend cell pellet after NaOH treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed with SYBR Green I dye master mix (Roche) and a CFX connect Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The amplicon was detected by EvaGreen Dye using LightCycler 480 instrument (Roche). PCR conditions were 5 min at 95 °C ...
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bioRxiv - Biochemistry 2021Quote: ... a linear DNA tether of the same length was amplified using a digoxigenin-modified primer instead of NTA (5’-DIG-NHS-TCCAAAGGTGAAGAACTGTTCACC, Integrated DNA Technologies, Inc. USA) to bind to anti-digoxigenin Fab fragment antibodies (11214667001, Roche).
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with a 1µg/ml solution of the DNA-dye DAPI (Roche) and mounted with the Slowfade antifade reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... uuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). All GFP+ Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). The labeled nuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2024Quote: ... nuclei were labeled with Hoechst dye and 0.2 U/μL Protector RNase inhibitor (Roche). All Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with a 1 µg/ml solution of the DNA-dye DAPI (Roche) and mounted with Slowfade antifade reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Additional RT-qPCR was performed using SYBR Green without the ROX reference dye (Roche Applied Science) and the QuantStudio 6 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the accumulation of products was monitored with the intercalating dye FastStart Universal SYBRGreen Master (ROX) (Roche). We quantified mRNA levels relative to three reference mRNAs ...
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Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibersbioRxiv - Cell Biology 2020Quote: ... Hoechst dye was added to label nuclei as well as 0.2 U/μl Protector RNase inhibitor (Roche). Fluorescently-labeled nuclei were purified via FACS (BD Aria ...
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bioRxiv - Neuroscience 2020Quote: Quantitative PCR was performed using SYBR Green I dye with LightCycler 480 technology (Roche, Branchburg, NJ, USA). The cDNA copy number was typically quantified against a ≥5 point ...
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bioRxiv - Immunology 2021Quote: ... The SYBR Green Dye detection system was used for quantitative real-time PCR on Light Cycler 480 (Roche). Controls consisting of ddH2O were negative for target and housekeeping genes ...
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bioRxiv - Genetics 2022Quote: qPCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618). Primers used for qPCR are described in Supplementary Table 4.
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... The SYBR Green Dye detection system was used for quantitative real-time PCR on Light Cycler 480 (Roche). Gene-specific primers (Microsynth ...
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bioRxiv - Cell Biology 2022Quote: ... Real time PCR was performed with SYBR green as the DNA binding dye (Roche Applied Science, Mannheim, Germany) on a 7900HT Fast Real-Time PCR System (Applied Biosystems under Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Realtime PCR was performed by using SYBR Green dye detection according to the manufacturer’s instruction (SYBR Green PCR Master Mix, Roche) on a LightCycler480 system ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... The qPCR was performed using 20 ng of cDNA product per reaction in the presence of Lightcycler 480 SYBR green dye (Roche). Lysosomal gene primers were designed using PrimerBLAST and ordered from Eurofins ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
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bioRxiv - Neuroscience 2022Quote: ... Samples for RT-PCR were run in duplicate using FAM-labelled probes or SYBR green dsDNA-intercalating fluorescent dye (Roche) in a StepOne Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of SWR was quantified by in-gel staining using the SYPRO Orange dye against a known concentration gradient of bovine serum albumin protein (Roche).
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bioRxiv - Biochemistry 2022Quote: ... 150 ng of U1 RNA (TMG or NAD cap) and 150 ng of HIV-1 mRNA were mixed with ResoLight dye (1x, Roche) and annealing buffer (10 mM Tris ...
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bioRxiv - Systems Biology 2020Quote: ... added to a qPCR mix (0.275 μL EvaGreen [31000, Biotium, Fremont, CA], 0.11 μL ROX Low Reference Dye [KK4602, Kapa Biosystems, Wilmington, MA], 0.115 μL water), and further cycled on a qPCR machine ...